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Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mo...

Alvarez, Catalina; Campos, Ana Claudia; Howard, Jonathan C; Loureiro, Joana; Müller, Urs Benedikt; Rodrigues, Ana Lina

Critical steps in resistance of mice against Toxoplasma gondii occur in the first 2 or 3 h after the pathogen has entered a cell that has been exposed to interferon γ (IFNγ). The newly formed parasitophorous vacuole is attacked by the IFNγ-inducible IRG proteins and disrupted, resulting in death of the parasite and necrotic death of the cell. Here we describe some techniques that we have used to describe and qu...

Date: 2020   |   Origin: ARCA - Access to Research and Communication Annals
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