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A magnetic nanoparticle-based microfluidic device fabricated using a 3D-printed...

Jóskowiak, Agnieszka; Nogueira, Catarina; Costa, Susana P.; Cunha, Alexandra; Freitas, Paulo P.; Carvalho, Carla Alexandra Oliveira Cunha Medeiros

Herein, A microfluidic device is described, produced with a 3D-printed master mould that rapidly separates and concentrates Escherichia coli directly from whole blood samples, enabling a reduction in the turnaround time of bloodstream infections (BSIs) diagnosis. Moreover, it promotes the cleansing of the blood samples whose complexity frequently hampers bacterial detection. The device comprises a serpentine mi...


Rapid and multiplex detection of nosocomial pathogens on a phage-based magnetor...

Cunha, Alexandra; Henriques, Raquel; Cardoso, Susana; Freitas, Paulo P.; Carvalho, Carla Alexandra Oliveira Cunha Medeiros

Abstract Nosocomial or hospital-acquired infections (HAIs) have a major impact on mortality worldwide. Enterococcus and Staphylococcus are among the leading causes of HAIs and thus are important pathogens to control mainly due to their increased antibiotic resistance. The gold-standard diagnostic methods for HAIs are time-consuming, which hinders timely and adequate treatment. Therefore, the development of fast...


Bacteriophage-receptor binding proteins for multiplex detection of Staphylococc...

Santos, Sílvio Roberto Branco; Cunha, Alexandra; Macedo, M.; Nogueira, Catarina; Brandão, Ana Catarina; Costa, Susana Patrícia Chaves

Health care-associated infections (HCAIs) affect hundreds of millions of patients, representing a significant burden for public health. They are usually associated to multidrug resistant bacteria, which increases their incidence and severity. Bloodstream infections (BSIs) are among the most frequent and life-threatening HCAIs, with Enterococcus and Staphylococcus among the most common isolated pathogens. The co...


Global analysis of seagrass restoration: the importance of large-scale planting

van Katwijk, Marieke M.; Thorhaug, Anitra; Marba, Nuria; Orth, Robert J.; Duarte, Carlos M.; Kendrick, Gary A.; Althuizen, Inge H. J.; Balestri, Elena

In coastal and estuarine systems, foundation species like seagrasses, mangroves, saltmarshes or corals provide important ecosystem services. Seagrasses are globally declining and their reintroduction has been shown to restore ecosystem functions. However, seagrass restoration is often challenging, given the dynamic and stressful environment that seagrasses often grow in. From our world-wide meta-analysis of sea...


A survey-based assessment of seagrass status, management and legislation in Europe

de los Santos, Carmen B.; Sigurðardóttir, Ragnhildur; Cunha, Alexandra; Cook, Kevan; Wiktor, Józef M.; Tatarek, Agnieszka; Santos, Rui

Seagrass meadows are ranked amongst the most productive ecosystems on Earth, providing high-value ecosystem services in coastal areas. Yet, seagrass habitats are declining worldwide at an unprecedented rate as a consequence of both anthropogenic and natural pressures, which suggests an inefficient management. Seagrasses are widespread along European coasts and the knowledge on their conservation status and mana...


Construir a Paz: visões interdisciplinares e internacionais sobre conhecimento ...

Pinto-Moreira, Paulo; Cunha, Pedro; Toscano, Ana; Barros, Carla; Toriz-Ramos, Cláudia; Jolluskin, Gloria; Costa-Leite, Isabel; Soares-Martins, José


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