74 documents found, page 1 of 8
A first-in-class inhibitor of homologous recombination DNA repair counteracts t...
Calheiros, Juliana; Silva, Rita; Barbosa, Filipa; Morais, João; Moura, Sara Reis; Almeida, Sofia; Fiorini, Elena; Mulhovo, Silva; Aguiar, Tatiana QuintaThe online version contains supplementary material available at: https://doi.org/10.1038/s41467-022-32178-3; Background Pancreatic ductal adenocarcinoma (PDAC) is among the cancer types with poorest prognosis and survival rates primarily due to resistance to standard-of-care therapies, including gemcitabine (GEM) and olaparib. Particularly, wild-type (wt)BRCA tumours, the most prevalent in PDAC, are more resist...
Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture
Aguiar, Tatiana Quinta; Leal, Tânia; Rodrigues, Diana G.; Abrunhosa, Luís; Oliveira, Carla; Domingues, LucíliaAvailable online 30 October 2024; Established chromatographic techniques for mycotoxin control in foodstuffs require prior sample enrichment and clean-up, typically achieved using immunoaffinity columns (IACs). Bovine serum albumin (BSA) has recently emerged as a cost-effective alternative to antibodies used in IACs. This study aimed at exploring the BSA domain II (BDII), which houses the primary binding site f...
Microplate fluorescence quenching for high throughput screening of affinity con...
Adalsteinsson, Heidar Már; Pinto, Lavínia Anjos Salazar Quartin; Aguiar, Tatiana Quinta; Teixeira, J. A.; Abrunhosa, LuísMeasurements of changes in fluorescence signal is one of the most commonly applied methods for studying protein-ligand affinities. These measurements are generally carried out using cuvettes in spectrofluorometers, which can only measure one sample at a time. This makes screening procedures for multiple ligands and proteins extremely laborious, as each protein must be measured with multiple ligand concentration...
Recombinant proteins: design, production and applications
Aguiar, Tatiana Quinta[Excerpt] Recombinant proteins are proteins encoded by recombinant DNA that has been cloned into an expression host. They can be manipulated in forms of existing proteins or entirely novel proteins generated by protein engineering. For instance, two or more existing proteins or peptides can be genetically fused to generate new multi-functional fusion proteins, being the use of purification/immobilization fusion...
Establishing sustainable bioproduction of lactones from metabolic engineering o...
Francisco, Miguel; Freitas, José Luís Silva; Moura, Clara D.; Costa, Carlos E.; Aguiar, Tatiana Quinta; Domingues, LucíliaIntroduction: Lactones are volatile organic compounds derived from lipid metabolism. They have a wide range of applications, notably as fragrances and flavours, with -decalactone being particularly valuable. Lactones significantly contribute to the smell and taste of fruits like strawberries and peaches, but traditional extraction methods have many disadvantages. Microbial production has emerged as a more susta...
Engineered Ashbya gossypii for single-cell oil production from non-detoxified E...
Francisco, Miguel; Aguiar, Tatiana Quinta; Marques, S.; Gírio, F.; Domingues, Lucília[Excerpt] Ashbya gossypii is a filamentous fungus industrially used for riboflavin production, a bioprocess in which downstream product recovery is facilitated by the ability of this fungus to undergo autolysis during the late stationary phase of growth or at low temperature [1]. In addition to riboflavin, engineered A. gossypii strains are capable of producing other compounds of interest for the food and feed ...
Toward cost-effective production of recombinant human serum albumin for use in ...
Rodrigues, Diana Isabel Gomes; Adalsteinsson, Heidar Már; Pinto, Lavínia; Aguiar, Tatiana Quinta; Oliveira, Carla; Abrunhosa, Luís; Domingues, LucíliaHuman serum albumin (HSA) is a monomeric multidomain macromolecule with wide clinical and biotechnological applications. Thus, there is a high demand for HSA, which is increasing due to new emerging applications. Among these, the extraordinary ligand binding capacity of HSA has been recently explored to capture mycotoxins from food and feed matrices. However, HSA is currently produced commercially primarily fro...
Single-cell oil production by engineered Ashbya gossypii from non-detoxified li...
Francisco, Miguel; Aguiar, Tatiana Quinta; Abreu, Gabriel; Marques, Susana; Gírio, Francisco; Domingues, LucíliaIn this work, microbial lipid production from non-detoxified Eucalyptus bark hydrolysate (EBH) with oleaginous xylose-utilizing Ashbya gossypii strains was explored. The best producing strain from a set of engineered strains was identified in synthetic media mimicking the composition of the non-detoxified EBH (SM), the lipid profile was characterized, and yeast extract and corn steep liquor (CSL) were pinpointe...
Recombinant protein purification and immobilization strategies based on peptide...
Aguiar, Tatiana Quinta; Domingues, LucíliaIron oxide and silica-based materials have emerged as attractive protein purification and immobilization matrices. His6 has been reported as an effective affinity tag for both iron oxide and silica. Here, the silica-binding tags CotB1p and Car9 were shown to work as effectively as iron oxide-binding tags. Using EGFP as a model protein, commercially available bare iron oxide (BIONs) or silicon dioxide (BSiNs) na...
Enhancing lactone biosynthesis in Ashbya gossypii: towards sustainable producti...
Costa, Carlos E.; Freitas, Luís; Francisco, Miguel Duarte Dias; Aguiar, Tatiana Quinta; Domingues, LucíliaLactones are volatile organic compounds sourced from lipid metabolism, with diverse applications, especially fragrances and flavours, being naturally present in fruits such as strawberries and peaches. Traditional lactone production is attained by biotransformation of hydroxylated fatty acids, which relies on limited precursor resources, such as vegetable or fish oils. However, the filamentous fungus Ashbya gos...