35 documents found, page 1 of 4

One-step purification of L-asparaginase from cell extracts using carbon xerogels

L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme with wide applications in the pharmaceutical sector and food processing industries. It is mainly used as a biotherapeutic for treating Acute Lymphoblastic Leukemia (ALL) and to reduce acrylamide formation in starchy compounds. Despite its relevance, current purification methods for microbial enzymes involve complex and expensive techniques. To overcome this drawb...

Supported ionic liquids to purify microbial L-Asparaginase

L-Asparaginase (ASNase) is a versatile enzyme that converts L-asparagine into ammonia and aspartic acid. This enzyme has applications in the food industry and health sector. However, high purity ASNase is required, resulting in high production costs. Therefore, in this work, two supported ionic liquids (SILs), specifically silica modified with dimethylbutylpropylammonium chloride ([Si][N3114]Cl) or triethylprop...

Recombinant laccase biosynthesis for efficient polydopamine coating

Laccases are versatile biocatalysts with interest for various industrial applications. This study reports the expression of Trametes versicolor laccase in Komagataella phaffii X33. The cultivation parameters (methanol and CuSO4 concentration, and temperature) for recombinant laccase production were studied in an orbital shaker. Enhanced laccase production was achieved by adding 1 % (v/v) methanol daily, supplem...

Influence of Choline Chloride on the Phase Equilibria and Partition Performance...

A series of polymeric aqueous biphasic systems (ABS) were determined using polyethylene glycol (PEG) and sodium polyacrylate (NaPA) with choline chloride ([Ch]Cl) as an adjuvant. The effect of (i) PEG and NaPA molecular weights, (ii) PEG functionalization, (iii) [Ch]Cl addition (at different concentrations), and (iv) temperature (25, 37 and 50 C) was evaluated through their ability to promote the two-phase sepa...

Supported ionic liquids for L-asparaginase downstream processing

L-asparaginase is an enzyme applied as a biopharmaceutical, as an acrylamide reduction agent and in the development of biosensors for the detection of L-asparagine (Nunes et al., 2020). L-asparaginase is widely distributed in nature, but microorganisms are the preferential source of production since they easily grow on inexpensive substrates (Lopes et al., 2017). For all the described applications, high levels ...

Purification of L-asparaginase through supported ionic liquid

L-asparaginase (ASNase) is an amidohydrolase enzyme applied as an anticancer agent in lymphoproliferative disorders treatment, as an acrylamide reduction agent in starch-rich foods cooked at high temperatures, and as a L-asparagine biosensor. ASNase is widely distributed in nature, e.g., microorganisms, plants and tissues of several animals. Nevertheless, most commercial ASNase are from recombinant microorganis...

Novel flow-through like mode platform for the purification of L-asparaginase

L-Asparaginase, an amidohydrolase enzyme applied in the pharmaceutical and food industries, is widely distributed in nature. Microorganisms are the preferential source of L-asparaginase production since they easily grow on inexpensive substrates. However, high levels of enzyme purity are required for all industrial applications, highlighting the need of developing cost-effective L-asparaginase purification proc...

A flow-through strategy using supported ionic liquids for L-asparaginase purifi...

L-asparaginase purification through silica-based supported ionic liquid-like ph...

L-asparaginase (ASNase) is an aminohydrolase enzyme widely applied as an anticancer biopharmaceutical in the treatment of lymphoproliferative disorders, as an acrylamide reduction agent in starch-rich foods cooked at high temperatures, and as a biosensor for the detection and monitoring of L-asparagine levels. While extensively distributed in nature, most commercial ASNase are from recombinant microorganisms, w...

Downstream processing of L-asparaginase through silica-based supported ionic li...

L-asparaginase (ASNase) is an amidohydrolase enzyme widely applied as an anticancer agent in the treatment of lymphoproliferative disorders, e.g., acute lymphoblastic leukemia (ALL), lymphomas, and natural killer cell tumors, as an acrylamide reduction agent in starch-rich foods cooked at high temperatures, and as a biosensor for L-asparagine detection and monitoring. ASNase is extensively distributed in nature...