16 documents found, page 1 of 2
Manufacture of food ingredients with biological activity for gut human health f...
Martínez-Gómez, Sergio; Santos-Pereira, Cátia; Pereira, Ana Catarina; Silva, Nuno R. da; Silvério, Sara Isabel Cruz; Alonso, José L.Lemon peels were subjected to hydrothermal treatment and the liquors obtained were processed following several downstream processing schemes obtaining a variety of products with different composition. Up to five products were generated, analysed for their saccharide and polyphenolic content and tested for their antimicrobial, antioxidant and anticancer (cytotoxicity) activities. Unfortunately, none of the produ...
Fodinibius alkaliphilus sp. nov., a moderately halophilic and alkaliphilic bact...
He, Yang; Simões, Marta Filipa Jesus Freitas; de la Haba, Rafael R.; Santos-Pereira, Cátia; Pereira-Sousa, Joana; Gomes, Joana S.A novel moderately halophilic and alkaliphilic Gram-stain-negative, strictly aerobic, bacterial strain (N2T) was isolated from an inland saltern in central Portugal. The taxonomic position of this isolate was determined based on polyphasic taxonomic and phylogenomic analysis. Phylogenetic analysis based on 16S rRNA gene sequences indicated that isolate N2T belongs to the genus Fodinibius, showing the highest si...
The fungicide cymoxanil impairs respiration in Saccharomyces cerevisiae via cyt...
Mendes, Filipa Diana Silva; Santos-Pereira, Cátia; Vieira, Tatiana F.; Pinto, Mélanie Martins; Castro, Bruno B.; Sousa, Sérgio F.; Sousa, Maria JoãoCymoxanil (CYM) is a widely used synthetic acetamide fungicide, but its biochemical mode of action remains elusive. Since CYM inhibits cell growth, biomass production, and respiration in Saccharomyces cerevisiae, we used this model to characterize the effect of CYM on mitochondria. We found it inhibits oxygen consumption in both whole cells and isolated mitochondria, specifically inhibiting cytochrome c oxidase...
Lactoferrin-loaded magnetic liposomes as potential photothermal agents for brea...
Andrade, Raquel G. D.; Santos-Pereira, Cátia; Ferreira, Débora Carina Gonçalves Abreu; Castanheira, Elisabete M. S.; Rodrigues, L. R.Nanoparticle-based drug delivery systems can afford an alternative treatment for breast cancer, overcoming conventional therapy’s limitations. In particular, superparamagnetic iron oxide nanoparticles entrapped in liposomes have gained significant interest in the biomedical field due to their exceptional properties, like enabling their accumulation at the intended target site when exposed to an external magneti...
Citrate-coated manganese and calcium ferrite nanoparticles as potential magneti...
Andrade, Raquel G. D.; Ferreira, Débora Carina Gonçalves Abreu; Veloso, Sérgio Rafael Silva; Santos-Pereira, Cátia; Castanheira, Elisabete M. S.In this work, manganese/calcium ferrite NPs coated with citrate, were synthesized by the sol-gel method, followed by calcination, and then characterized regarding their crystalline structure (by X-ray diffraction, XRD), size and shape (by Transmission Electron Microscopy, TEM), hydrodynamic size and zeta potential (by Dynamic Light Scattering, DLS), and heating efficiency (measuring the Specific Absorption Rate...
Finding novel genes coding for eco-friendly surfactants from hypersaline Iberia...
Santos-Pereira, Cátia; Gomes, Joana S.; Sousa, Joana; Simões, Marta F.; Franco-Duarte, Ricardo; Chaves, Susana R.; Silvério, Sara Isabel CruzSurfactants are tensioactive chemical compounds extensively used worldwide in a myriad of industrial sectors and in our daily lives, being present in numerous products including cosmetics, detergents, fabric softeners, toothpaste, among many others. Millions of tonnes of surfactants are thus manufactured every year. Most commercially available surfactants are non-renewable petroleum-based compounds that can hav...
Heterologous expression and structure prediction of a xylanase identified from ...
Sousa, Joana; Santos-Pereira, Cátia; Dias, Joana Sofia Gomes; Costa, Ângela Maria Araújo; Santos, Andréia O.; Franco-Duarte, RicardoXylanases are key biocatalysts in the degradation of the 1,4glycosidic linkages in the xylan backbone of hemicellulose. These enzymes are potentially applied in a wide range of bioprocessing industries under harsh conditions. Metagenomics has emerged as powerful tools for the bioprospection and discovery of interesting bioactive molecules from extreme ecosystems with unique features, such as high temperatures. ...
Enhancement of acetate-induced apoptosis of colorectal cancer cells by cathepsi...
Alves, Sara; Santos-Pereira, Cátia; Oliveira, Cláudia S. F.; Preto, Ana; Chaves, Susana R.; Côrte-Real, ManuelaColorectal cancer (CRC) is a leading cause of death worldwide. Conventional therapies are available with varying effectiveness. Acetate, a short-chain fatty acid produced by human intestinal bacteria, triggers mitochondria-mediated apoptosis preferentially in CRC but not in normal colonocytes, which has spurred an interest in its use for CRC prevention/therapy. We previously uncovered that acetate-induced mitoc...
Metagenomic approach to identify genes encoding for glycoside hydrolases in com...
Sousa, Joana; Santos-Pereira, Cátia; Costa, Ângela Maria Araújo; Guiet, Andréia Oliveira Santos; Franco-Duarte, Ricardo; Rodrigues, L. R.Metagenomics involves the study of the genomic DNA from a set of microorganisms present in a particular environmental sample. This approach has emerged as a promising culture-independent technique to explore the diversity and function of microbiomes, allowing the discovery of novel biochemical compounds, namely enzymes with high potential for industrial applications. Composting habitats are characterized by a h...
Identification of lignocellulose-degrading enzymes using metagenomic approaches
Sousa, Joana; Santos-Pereira, Cátia; Costa, Ângela Maria Araújo; Santos, Andréia O; Gomes, Joana; Rodrigues, Joana Lúcia Lima CorreiaComposting units which handle lignocellulosic residues are suitable sources of novel and promising lignocellulose-degrading enzymes such as cellulases, xylanases and amylases. These enzymes have practical application in many industries where lignocellulose is converted into several added-value bioproducts. However, the effective conversion of lignocellulose by a sustainable process is currently incomplete. Ther...