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Screening Wild Birds for Tick-Borne Zoonotic Pathogens in Portugal

Loureiro, F; Mesquita, JR; Cardoso, L; Santos-Silva, S; Moreira, G; Bento, JT; Soeiro, V; Gonçalves, A; Silva, F; Barradas, PF; Matos, AC; Matos, M

Wild birds may be involved in the transmission of agents of infectious diseases, including zoonoses, a circumstance which raises a number of public and animal health issues. Migratory bird species play a significant role in the introduction of tick-borne pathogens to new geographic areas, contributing to the dissemination of various etiological agents. This preliminary study aimed to assess the occurrence of fo...


Detection of biliary trematodes in sheep and goats from northern and central Po...

Santos-Silva, S; Cruz, AVS; Gomes da Silva, P; Bento, JT; Almeida, D; Barradas, PF; Cardoso, L; Mesquita, JR

Fasciolosis and dicrocoeliosis are severe parasitic infections that result in substantial economic damages to livestock farming globally as a consequence of reduced productivity and viscera condemnation. Molecular tests such as polymerase chain reaction (PCR) can detect Fasciola hepatica and Dicrocoelium dendriticum DNA with high sensitivity and specificity. In this study, we aimed to assess the presence of F. ...


Seroprevalence study of brucellosis in wild boar hunted for private consumption...

Ruano, ZM; Mateus, TL; Chorense, A; Santos-Silva, S; Vieira-Pinto, M

Brucellosis is an important infectious disease caused by bacteria of the genus Brucella. In the northeast region of Portugal, infection with Brucella melitensis is endemic in small ruminants, and there are also humans’ cases. However, the epidemiological role of the wild boar in the dynamics of this disease in this region is unknown. In this study, a total of 332 blood samples were collected from wild boar hunt...


Detection of hepatitis E virus genotype 3 in an Algerian mouse (Mus spretus) in...

Santos-Silva, S; Moraes, DFDSD; López-López, P; Paupério, J; Queirós, J; Rivero-Juarez, A; Lux, L; Ulrich, RG; Gonçalves, HMR; Van der Poel, WHM

Virus monitoring in small mammals is central to the design of epidemiological control strategies for rodent-borne zoonotic viruses. Synanthropic small mammals are versatile and may be potential carriers of several microbial agents. In the present work, a total of 330 fecal samples of small mammals were collected at two sites in the North of Portugal and screened for zoonotic hepatitis E virus (HEV, species Pasl...


A Thorny Tale of Parasites: Screening for Enteric Protozoan Parasites in Hedgeh...

Gomes-Gonçalves, S; Santos-Silva, S; Cruz, AVS; Rodrigues, C; Soeiro, V; Barradas, P; Mesquita, JR

Enteric protozoan parasites, such as Blastocystis sp., Balantioides coli, Cryptosporidium spp., and Giardia duodenalis, may have implications for both animal and human health.Transmitted through the fecal–oral route, these parasites cause symptoms such as diarrhea, abdominal pain, and weight loss. This study investigated the presence of these enteric protozoan parasites and genetically characterized them in hed...


Multicentric Study on Enteric Protists Occurrence in Zoological Parks in Portugal

Mega, J; Moreira, R; Moreira, G; Silva-Loureiro, A; Gomes da Silva, P; Istrate, C; Santos-Silva, S; Rivero-Juarez, A; Carmena, D; Mesquita, JR

Parasitic infections of the gastrointestinal tract of domestic animals play a major role in the transmission of disease, which in turn may result in financial and productive losses. Notwithstanding, studies on the burden and distribution of diarrheagenic protists in zoological gardens are still insufficient. Given the close animal-animal and animal-human interaction in these settings, Public Health concerns und...


Hepatitis E virus in wild and domestic rabbits from Portugal: a combined molecu...

Santos-Silva, S; Santos, N; López-López, P; Nascimento, MSJ; Gonçalves, HMR; Van der Poel, WHM; Rivero-Juarez, A; Mesquita, JR

Hepatitis E virus (HEV), species Paslahepevirus balayani, poses a global public health threat, especially in developing countries, by causing acute enterically transmitted hepatitis. HEV infects various mammalian hosts and belongs to the genus Paslahepevirus in the family Hepeviridae. While swine are recognized as the main hosts of HEV, rabbits, which can also be affected by swine HEV-3 related strains, serve a...


Hunting for Answers: Assessing Brucella spp. Seroprevalence and Risks in Red De...

Pires, H; Cardoso, L; Lopes, AP; Fontes, MDC; Santos-Silva, S; Matos, M; Pintado, C; Roque, N; Fonseca, LF; Morgado, I; Dias, AS; Figueira, L; Matos, AC

Between 2016 and 2023, a cross-sectional study was conducted in the central region of Portugal in order to better understand the epidemiology and public health risks resulting from the handling and consumption of game animals infected with Brucella spp. The seroprevalence and risk factors for Brucella spp. seropositivity were evaluated. Antibodies against Brucella spp. were determined using a commercial enzyme-...


Serological and Molecular Survey of Hepatitis E Virus in Small Ruminants from C...

Santos-Silva, S; Romalde, JL; Bento, JT; Cruz, AVS; López-López, P; Gonçalves, HMR; Van der Poel, WHM; Nascimento, MSJ; Rivero-Juarez, A; Mesquita, JR

Hepatitis E virus (HEV) is currently recognized as an emerging problem and a growing concern for public health in developed countries, with HEV infections mainly attributable to foodborne transmission of HEV-3. The zoonotic HEV genotype 3 infects a wide range of mammalian hosts, with swine considered as the primary host. This study investigates the occurrence of HEV among small ruminants in Portugal. The primar...


Molecular evidence of sporadic Coxiella burnetii excretion in sheep milk, centr...

Pires, H; Santos-Silva, S; Cruz, AVS; Cardoso, L; Lopes, AP; Pereira, MA; Nóbrega, C; Mega, AC; Santos, C; Cruz, R; Esteves, F; Vala, H; Matos, AC

Coxiella burnetii is the etiologic agent of Q fever, a worldwide zoonosis. Cattle, sheep and goats are considered the main reservoirs of the disease. Transmission to humans occurs mainly through the inhalation of infectious aerosols from milk, faeces, urine, and birth products from infected ruminants. In this study, a 2-year longitudinal approach was performed to ascertain the excretion of C. burnetii in bulk t...


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