13 documents found, page 1 of 2

Interaction of polypeptides with hair in different reducing environments

Innovation in product development and cosmetic treatments is essential to meet the demands of the hair care industry. This work studied the influence of reducing environments in the interaction of polypeptides (KP peptide, ELP-KP and BSK recombinant proteins and hydrolyzed keratin) with damaged hair, to recover the hair damage. The reducing conditions resulted from thioglycolic acid and thioanisole addition. Th...

AI, docking, and molecular dynamics to track the binding of structural peptides...

The present work shows a computational approach to assess the interactions of different nature-inspired peptides with hair keratin models. An updated keratin model was validated, and comparisons with previous models were traced, thereby highlighting the time-evolution of both the model and the in silico methods. Importantly, the computational methodology presented here allows for the study of peptide-protein in...

Development of bio-hybrid piezoresistive nanocomposites using silk-elastin prot...

Recombinant silk-elastin-like protein (SELP)/carbon nanotubes (CNTs) nanocomposite films with different amounts of CNTs (1, 3 and 6wt%) were prepared by solvent casting. The produced films were stabilized by exposure to methanol that induces an increase of the -structure content. The CNTs were homogeneously distributed into the SELP matrix and did not induce significant alterations into its chemical structure. ...

Exploring the properties of genetically engineered silk-elastin-like protein films

Free standing films of a genetically engineered silk-elastin-like protein (SELP) were prepared using water and formic acid as solvents. Exposure to methanol-saturated air promoted the formation of aggregated β-strands rendering aqueous insolubility and improved the mechanical properties leading to a 10-fold increase in strain-to-failure. The films were optically clear with resistivity values similar to natural ...

Optimising the production of a silk-elastin-like protein in E. coli: overcoming...

Silk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels. Here we describe the production optimisation for a novel recently described SELP in the pETE. coli BL21(DE3) expression system. Bo...

Development of new chimeric proteins for tissue engineering

Tissue engineering is an emerging !eld focused on the development of novel bioactive multifunctional materials that can be used to replace damaged and failing tissues. However, these biomaterials often present several problems such as loss of mechanical and/or biological properties and adverse immune responses. The use of natural polymers, such as proteins, provides a promising solution for these drawbacks. Wit...

Key stress factors and parameters for production optimisation of silk-elastin-l...

Silk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels. We have recently synthesised a series of novel silk-elastin-like proteins and here we will describe the optimisation of the produc...

Antibacterial and antifungal activity of a new solvent-cast film based on a fun...

Batch production of a silk-elastin-like protein in E. coli BL21(DE3) : key para...

Background: Silk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels. Here we describe the batch production optimisation for a novel recently described SELP in the pET-E. coli BL21(DE3) ex...

Optimising a cell factory system for the bioproduction of silk-elastin-like pol...

Silk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels. Here we describe the production optimisation for a novel recently described SELP in the pETE. coli BL21(DE3) expression system. Bo...