30 documents found, page 1 of 3
Influence of genetic variants of oxidative stress related genes on prognosis an...
Alves, Raquel; Ventura, Filipa; Jorge, Joana; Ribeiro, Ilda P.; Marques, Gilberto; Coucelo, Margarida; Diamond, Joana; Freitas-Tavares, PauloChronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the presence of BCR-ABL1 oncogene. Oxidative stress conditions are crucial in tumor development and progression. The NRF2, codified by NFE2L2 gene, plays a central role in redox balance, promoting the transcription of cytoprotective and antioxidant genes such as SOD2, GPX1 and CAT. The action of this transcription factor is regulat...
Genetic variants in oxidative stress-related genes and their impact on prognosi...
Alves, Raquel; Ventura, Filipa; Jorge, Joana; Marques, Gilberto; Coucelo, Margarida; Diamond, Joana; Oliveiros, Bárbara; Pereira, AméliaChronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasia characterized by the BCR::ABL1 fusion gene, which codifies the BCR-ABL protein with increased tyrosine kinase activity. Despite the clinical results for the outstanding tyrosine kinase inhibitors (TKIs), drug resistance is a problem in CML management. Genetic variants that alter redox homeostasis by changing antioxidant enzyme expression or...
Batimastat Induces Cytotoxic and Cytostatic Effects in In Vitro Models of Hemat...
Alves, Raquel; Pires, Ana; Jorge, Joana; Balça-Silva, Joana; Gonçalves, Ana Cristina; Sarmento-Ribeiro, Ana BelaThe role of metalloproteinases (MMPs) in hematological malignancies, like acute myeloid leukemia (AML), myelodysplastic neoplasms (MDS), and multiple myeloma (MM), is well-documented, and these pathologies remain with poor outcomes despite treatment advancements. In this study, we investigated the effects of batimastat (BB-94), an MMP inhibitor (MMPi), in single-administration and daily administration schemes i...
Zinc: From Biological Functions to Therapeutic Potential
Costa, Maria Inês; Sarmento-Ribeiro, Ana Bela; Gonçalves, Ana CristinaThe trace element zinc (Zn) displays a wide range of biological functions. Zn ions control intercellular communication and intracellular events that maintain normal physiological processes. These effects are achieved through the modulation of several Zn-dependent proteins, including transcription factors and enzymes of key cell signaling pathways, namely those involved in proliferation, apoptosis, and antioxida...
Platelet Membrane Proteins as Pain Biomarkers in Patients with Severe Dementia
Ribeiro, Hugo; Alves, Raquel; Jorge, Joana; Gonçalves, Ana Cristina; Sarmento-Ribeiro, Ana Bela; Veríssimo, Manuel T.; Dourado, MaríliaPain is one of the most frequent health problems, and its evaluation and therapeutic approach largely depend on patient self-report. When it is not possible to obtain a self-report, the therapeutic decision becomes more difficult and limited. This study aims to evaluate whether some membrane platelet proteins could be of value in pain characterization. To achieve this goal, we used 53 blood samples obtained fro...
Alvespimycin Inhibits Heat Shock Protein 90 and Overcomes Imatinib Resistance i...
Alves, Raquel; Santos, Diogo; Jorge, Joana; Gonçalves, Ana Cristina; Catarino, Steve; Girão, Henrique; Melo, Joana Barbosa; Sarmento-Ribeiro, Ana BelaHeat shock protein 90 (HSP90) facilitates folding and stability and prevents the degradation of multiple client proteins. One of these HSP90 clients is BCR-ABL, the oncoprotein characteristic of chronic myeloid leukemia (CML) and the target of tyrosine kinase inhibitors, such as imatinib. Alvespimycin is an HSP90 inhibitor with better pharmacokinetic properties and fewer side effects than other similar drugs, b...
Monocytes in the Characterization of Pain in Palliative Patients with Severe De...
Ribeiro, Hugo; Alves, Raquel; Jorge, Joana; Gonçalves, Ana Cristina; Sarmento-Ribeiro, Ana Bela; Veríssimo, Manuel T.; Andrade, José PauloIn assessing and managing pain, when obtaining a self-report is impossible, therapeutic decision-making becomes more challenging. This study aimed to investigate whether monocytes and some membrane monocyte proteins, identified as a cluster of differentiation (CD), could be potential non-invasive peripheral biomarkers in identifying and characterizing pain in patients with severe dementia. We used 53 blood samp...
Parthenolide Induces ROS-Mediated Apoptosis in Lymphoid Malignancies
Jorge, Joana; Neves, Joana; Alves, Raquel; Geraldes, Catarina; Gonçalves, Ana Cristina; Sarmento-Ribeiro, Ana BelaLymphoid malignancies are a group of highly heterogeneous diseases frequently associated with constitutive activation of the nuclear factor kappa B (NF-κB) signaling pathway. Parthenolide is a natural compound used to treat migraines and arthritis and found to act as a potent NF-κB signaling inhibitor. This study evaluated in vitro parthenolide efficacy in lymphoid neoplasms. We assessed parthenolide metabolic ...
AZD-7648, a DNA-PK Inhibitor, Induces DNA Damage, Apoptosis, and Cell Cycle Arr...
Lapa, Beatriz Santos; Costa, Maria Inês; Figueiredo, Diana; Jorge, Joana; Alves, Raquel; Monteiro, Ana Raquel; Serambeque, Beatriz; Laranjo, MafaldaThe non-homologous end joining pathway is vital for repairing DNA double-strand breaks (DSB), with DNA-dependent protein kinase (DNA-PK) playing a critical role. Altered DNA damage response (DDR) in chronic (CML) and acute myeloid leukemia (AML) offers potential therapeutic opportunities. We studied the therapeutic potential of AZD-7648 (DNA-PK inhibitor) in CML and AML cell lines. This study used two CML (K-56...
Cold Atmospheric Plasma Apoptotic and Oxidative Effects on MCF7 and HCC1806 Hum...
Almeida-Ferreira, Catarina; Silva-Teixeira, Rafael; Gonçalves, Ana Cristina; Marto, Carlos Miguel; Sarmento-Ribeiro, Ana Bela; Caramelo, FranciscoBreast cancer (BC) is a malignant neoplasia with the highest incidence and mortality rates in women worldwide. Currently, therapies include surgery, radiotherapy, and chemotherapy, including targeted therapies in some cases. However, treatments are often associated with serious adverse effects. Looking for new options in BC treatment, we evaluated the therapeutic potential of cold atmospheric plasma (CAP) in tw...