4 documents found, page 1 of 1
Comparison of resazurin microtiter assay performance and BACTEC MGIT 960 in the...
Miyata,Marcelo; Pavan,Fernando Rogério; Sato,Daisy Nakamura; Marino,Leonardo Biancolino; Hirata,Mario Hiroyuki; Cardoso,Rosilene FressatiWe assessed the performance of REMA in comparison with BACTEC MGIT 960 in the susceptibility testing of 80 Mycobacterium tuberculosis clinical isolates from Clemente Ferreira Institute against four drugs. REMA proved to be a rapid and accurate method, providing excellent correlation with BACTEC MGIT 960, with the exception of results for the ethambutol drug.
Phenotypic and genotypic characterization of Rhodococcus equi isolated from sputum
Silva,Paulo da; Santos,Adolfo Carlos Barreto; Sato,Daisy Nakamura; Silva,Jaqueline Otero; Medeiros,Marta Inês Cazentini; Carneiro,Ana Maria MachadoINTRODUCTION: Rhodococcus equi is an opportunistic pathogen, causing rhodococcosis, a condition that can be confused with tuberculosis. Often, without identifying M. tuberculosis, physicians initiate empiric treatment for tuberculosis. R. equi and M. tuberculosis have different susceptibility to drugs. Identification of R. equi is based on a variety of phenotypic, chromatographic, and genotypic characteristics....
Evaluation of anti-Mycobacterium tuberculosis activity of Campomanesia adamanti...
Pavan,Fernando Rogério; Leite,Clarice Queico Fujimura; Coelho,Roberta Gomes; Coutinho,Isabel Duarte; Honda,Neli Kika; Cardoso,Claudia Andréa LimaThe anti-Mycobacterium tuberculosis activity of Campomanesia adamantium fruits extracts were evaluated. Six compounds, identified as flavanones and chalcones were quantified by HPLC-DAD-UV. Promising antitubercular activity was observed with ethyl acetate extract (MIC 62.5 µg/mL) and their fractions (MIC values ranging from 39 to above 250 µg/mL). The better MIC result of 39 µg/mL was associated with two fracti...
Triterpenes and antitubercular activity of Byrsonima crassa
Higuchi,Celio Takashi; Pavan,Fernando Rogério; Leite,Clarice Queico Fujimura; Sannomiya,Miriam; Vilegas,Wagner; Leite,Sergio Roberto de AndradeWe evaluated the potential antitubercular activity of triterpenes obtained from leaves and bark of Byrsonima crassa. From chloroform extracts of the leaves, by bioassay-guided fractionation, we obtained mixtures of known triterpenes: α-amyrin, β-amyrin and their acetates, lupeol, oleanolic acid, ursolic acid and α-amyrinone. Tested against Mycobacterium tuberculosis, the triterpenes exhibited minimum inhibitory...