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The Aspergillus nidulans signalling mucin MsbA regulates starvation responses, ...

Brown, Neil Andrew; dos Reis, Thaila Fernanda; Goinski, Amanda Beatriz; Savoldi, Marcela; Menino, João Filipe Marques Almeida Ferreira

In the heterogeneous semi-solid environment naturally occupied by lignocellulolytic fungi the majority of nutrients are locked away as insoluble plant biomass. Hence, lignocellulolytic fungi must actively search for, and attach to, a desirable source of nutrients. During growth on lignocellulose a period of carbon deprivation provokes carbon catabolite derepression and scavenging hydrolase secretion. Subsequent...


Functional Characterization of an Aspergillus fumigatus Calcium Transporter (Pm...

Dinamarco, Taisa Magnani; Freitas, Fernanda Zanolli [UNESP]; Almeida, Ricardo S.; Brown, Neil Andrew; dos Reis, Thaila Fernanda

Made available in DSpace on 2013-08-28T14:12:07Z (GMT). No. of bitstreams: 1 WOS000305335800062.pdf: 1062294 bytes, checksum: f286e05f0c5cc5f96d3ea2622d7596c5 (MD5); Made available in DSpace on 2013-09-30T19:07:40Z (GMT). No. of bitstreams: 2 WOS000305335800062.pdf: 1062294 bytes, checksum: f286e05f0c5cc5f96d3ea2622d7596c5 (MD5) WOS000305335800062.pdf.txt: 73864 bytes, checksum: 8502f99cf5690998cef4024bcc752f3c...

Date: 2014   |   Origin: Oasisbr

Identification and complete sequencing of novel human transcripts through the u...

Ferreira, Elisa N.; Pires, Lilian C.; Parmigiani, Raphael B.; Bettoni, Fabiana; Puga, Renato D.; Pinheiro, Daniel G.; Andrade, Luís Eduardo C.

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:21:13Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:38:23Z : No. of bitstreams: 1 2-s2.0-20044376290.pdf: 540809 bytes, checksum: a96333f2fc93cfef3f7a5af233037c93 (MD5); Made available in DSpace on 2014-05-27T11:21:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-12-01; The correct identification of all h...

Date: 2014   |   Origin: Oasisbr

Functional characterization of a xylose transporter in Aspergillus nidulans

Rodrigues, Fernando José dos Santos; Menino, João F.; Colabardini, Ana Cristina; Ries, Laure Nicolas Annick; Brown, Neil Andrew

BACKGROUND: The production of bioethanol from lignocellulosic feedstocks will only become economically feasible when the majority of cellulosic and hemicellulosic biopolymers can be efficiently converted into bioethanol. The main component of cellulose is glucose, whereas hemicelluloses mainly consist of pentose sugars such as D-xylose and L-arabinose. The genomes of filamentous fungi such as A. nidulans encode...


Identification of metabolic pathways influenced by the G-protein coupled recept...

Souza, Wagner R. de; Morais, Enyara Rezende; Krohn, Nadia Graciele; Savoldi, Marcela; Goldman, Maria Helena S.; Rodrigues, Fernando; Caldana, Camila

Heterotrimeric G-protein-mediated signaling pathways play a pivotal role in transmembrane signaling in eukaryotes. Our main aim was to identify signaling pathways regulated by A. nidulans GprB and GprD G-protein coupled receptors (GPCRs). When these two null mutant strains were compared to the wild-type strain, the DeltagprB mutant showed an increased protein kinase A (PKA) activity while growing in glucose 1% ...


Identification of glucose transporters in Aspergillus nidulans

Reis, Thaila Fernanda dos; Menino, João F.; Pedro Bom, Vinícius Leite; Brown, Neil Andrew; Colabardini, Ana Cristina; Savoldi, Marcela

o characterize the mechanisms involved in glucose transport, in the filamentous fungus Aspergillus nidulans, we have identified four glucose transporter encoding genes hxtB-E. We evaluated the ability of hxtB-E to functionally complement the Saccharomyces cerevisiae EBY.VW4000 strain that is unable to grow on glucose, fructose, mannose or galactose as single carbon source. In S. cerevisiae HxtB-E were targeted ...


Identification of metabolic pathways influenced by the G-protein coupled recept...

de Souza, Wagner R; Morais, Enyara Rezende; Krohn, Nadia Graciele; Savoldi, Marcela; Goldman, Maria Helena S; Rodrigues, Fernando; Caldana, Camila

Heterotrimeric G-protein-mediated signaling pathways play a pivotal role in transmembrane signaling in eukaryotes. Our main aim was to identify signaling pathways regulated by A. nidulans GprB and GprD G-protein coupled receptors (GPCRs). When these two null mutant strains were compared to the wild-type strain, the ΔgprB mutant showed an increased protein kinase A (PKA) activity while growing in glucose 1% and ...


The conserved and divergent roles of carbonic anhydrases in the filamentous fun...

Han, Kap-Hoon; Chun, Yoon-Hee; Figueiredo, Bárbara de Castro Pimentel; Soriani, Frederico Marianetti; Savoldi, Marcela; Almeida, Agostinho João Ramalho

Carbon dioxide (CO(2)) and its hydration product bicarbonate (HCO(3)(-)) are essential molecules in various physiological processes of all living organisms. The reversible interconversion between CO(2) and HCO(3)(-) is in equilibrium. This reaction is slow without catalyst, but can be rapidly facilitated by Zn(2+)-metalloenzymes named carbonic anhydrases (CAs). To gain an insight into the function of multiple c...


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