19 documents found, page 1 of 2
Vascular and ventilatory mechanical responses in three different stages of pulm...
Figueira,Rebeca Lopes; Costa,Karina Miura da; Marsico,Ana Laura; Milani,Thamires Melchiades da Silva; Gonçalves,Walusa Assad; Borges,Marcos de CarvalhoAbstract Purpose: To evaluate the vascular ventilatory response in different stages of lung development and to compare them to the neonates with congenital diaphragmatic hernia (CDH) in a rabbit model. Methods: New Zealand rabbits were divided into 8 groups (n=5): E25, E27, E30, and CDH. All groups were ventilated on a FlexiVent (Scireq, Montreal, QC, Canada), compounding the other 4 groups. The CDH surgery was...
Manejo pré-natal da hérnia diafragmática congênita: presente, passado e futuro
Gallindo,Rodrigo Melo; Gonçalves,Frances Lanhellas; Figueira,Rebeca Lopes; Sbragia,LourençoA hérnia diafragmática congênita é um defeito de formação do diafragma que acomete entre 1:2.000 e 1:4.000 nascidos vivos e constitui 8% das principais anomalias congênitas. Avanços médicos nos últimos 30 anos envolvendo diagnóstico pré-natal, intervenção fetal, manejo clinico e cirúrgico neonatal têm mudado a sobrevivência dos seus portadores. A evolução histórica desses avanços ajuda a compreender o esforço n...
L-FABP and I-FABP expression in newborn rats changes inversely in the model of ...
Mitidiero,Luís Felipe Tsuyoshi; Simões,Ana Leda Bertoncini; Gonçalves,Frances Lilian Lanhellas; Figueira,Rebeca Rodrigues Lopes RoslindoPURPOSE: To determine the expression of hepatic L-FABP and intestinal I-FABP in an experimental model of necrotizing enterocolitis (NEC) in neonatal rats.METHODS: Newborn Sprague-Dawley rats were divided into four groups: Control (C1) - exclusive breastfeeding at the first and sixth procedures (C6), NEC1 - fed formula milk and submitted to hypoxia and hypothermia at the first and sixth procedures (NEC6). The ne...
Standardization of pulmonary ventilation technique using volume-controlled vent...
Gallindo,Rodrigo Melo; Gonçalves,Frances Lilian Lanhellas; Figueira,Rebeca Lopes; Simões,Ana Leda Bertoncini; Sbragia,LourençoOBJECTIVE: To standardize a technique for ventilating rat fetuses with Congenital Diaphragmatic Hernia (CDH) using a volume-controlled ventilator.METHODS: Pregnant rats were divided into the following groups: a) control (C); b) exposed to nitrofen with CDH (CDH); and c) exposed to nitrofen without CDH (N-). Fetuses of the three groups were randomly divided into the subgroups ventilated (V) and non-ventilated (N...
The role of gut-liver axis in the restriction of intrauterine growth in a model...
Bueno,Márcia Pereira; Gonçalves,Frances Lilian Lanhellas; Guadagnini,Dioze; Barini,Ricardo; Saad,Mario José Abdalla; Gallindo,Rodrigo MeloPURPOSE: To evaluate the intrauterine growth restriction (IUGR) by the expression of IR-β, IRS-1, IRS-2, IGF-IRβ and Ikappaβ in experimental model of gastroschisis. METHODS: Pregnant rats at 18.5 days of gestation were submitted to surgery to create experimental fetal gastroschisis (term = 22 days) were divided in three groups: gastroschisis (G), control (C) and sham (S). Fetuses were evaluated for body weight ...
Corticosteroid effect upon intestinal and hepatic interleukin profile in a gast...
Gonçalves,Frances Lilian Lanhellas; Bittencourt,Daniel Guimarães; Velloso,Lício Augusto; Schmidt,Augusto Frederico; Gallindo,Rodrigo MeloPURPOSE: To evaluate the effect of corticosteroids on intestinal and liver interleukin profile in an experimental model of gastroschisis in fetal rats. METHODS: Sprague-Dawley rats at 19.5 days of gestation had its fetuses operated for the creation of gastroschisis. Two groups of fetuses were studied with and without maternal administration of dexamethasone. Each group was composed of fetuses who underwent gast...
Effect of nitrofen in the final stages of development of the diaphragm muscle i...
Gonçalves,Frances Lilian Lanhellas; Oliveira,Fábio Santana de; Schmidt,Augusto Frederico; Pereira,Luís Antônio Violin Dias; Gallindo,Rodrigo MeloPURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4'nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, w...
Validation of protocol of experimental necrotizing enterocolitis in rats and th...
Gonçalves,Frances Lilian Lanhellas; Gallindo,Rodrigo Melo; Soares,Lucas Manoel Mangueira; Figueira,Rebeca Lopes; Volpe,Fábio Antônio PerecimPURPOSE: To describe the difficulties of implementing the protocol of experimental necrotizing enterocolitis (NEC) in order to obtain a larger number of newborns affected with the disease and a lower mortality. METHODS: Term Sprague-Dawley newborns rats (22 days) were divided into four groups of 12 fetuses each (n = 48): EC - breastfed newborns; IH - breastfed newborns and subjected to a stress protocol by isch...
Oclusão traqueal para fetos com hérnia diafragmática esquerda grave isolada: um...
Peralta,Cleisson Fábio Andrioli; Sbragia,Lourenço; Bennini,João Renato; Cavalli,Ricardo Carvalho; Rousselet,Monique Sampaio; Barini,RicardoOBJETIVO: Comparar a sobrevida pós-natal de fetos com hérnia diafragmática congênita esquerda grave isolada, os quais foram submetidos à oclusão traqueal, com a de controles não randomizados contemporâneos. MÉTODOS: Estudo experimental não randomizado e controlado, conduzido de abril de 2007 a setembro de 2011. Fetos portadores de hérnia diafragmática congênita esquerda isolada com herniação hepática e relação ...
Tratamento das malformações fetais intraútero
Sbragia,LourençoRESUMO Aproximadamente 1% das gestações apresenta anomalias estruturais. Durante as últimas três décadas, vários estudos experimentais em animais de grande porte associados ao avanço tecnológico dos equipamentos de imagem diagnóstica e de fetoscopia permitiram grande evolução no conhecimento da fisiopatologia de vários defeitos congênitos. Tais conhecimentos aplicados na correção intraútero das anomalias transf...