7 documents found, page 1 of 1
Evaluation of Toxocara canis Glycosylated TES Produced in Pichia pastoris for I...
Santos,Lucas Moreira dos; Cerqueira,Michele Pepe; Gaboardi,Giana Carla; Magalhães,Carolina Georg; Donassolo,Rafael Amaral; Rodrigues,Rafael RodriguesAbstract Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products. As an alternative, yeast such as Pichia pastoris have secretory mechanisms, which could diminish the cost and time for production. This study aimed to produce recombinant proteins in Pichia pastoris and verify the...
Evaluation of Pregnant and Postpartum Women’s Knowledge about Toxoplasmosis in ...
Lehmann,Lis Maurente; Santos,Paula Costa; Scaini,Carlos JamesAbstract Introduction Toxoplasmosis a parasitic zoonosis of global distribution, responsible for disorders during gestation can cause fetal death or congenital anomalies. Objective To evaluate the knowledge of toxoplasmosis among pregnant and postpartum women treated at the University Hospital of the city of Rio Grande, Rio Grande do Sul, Brazil. Methods This was a cross-sectional study of 100 pregnant and post...
Determination of IgG avidity in BALB/c mice experimentally infected with Toxoca...
Schoenardie,Elizandra Roselaine; Scaini,Carlos James; Avila,Luciana Farias da Costa de; Sperotto,Rita Leal; Borsuk,Sibele; Felicetti,Cristine Dias PiresToxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples...
Vertical transmission of Toxocara canis in successive generations of mice
Schoenardie,Elizandra Roselaine; Scaini,Carlos James; Pepe,Michele Soares; Borsuk,Sibele; Avila,Luciana Farias da Costa de; Villela,MarcosVisceral toxocariasis is a neglected zoonosis caused by Toxocara canis larvae in unusual hosts. In dogs, the definitive host, the infection occurs mainly through transplacental and transcolostral transmission. Studies on experimental models have shown that vertical transmission may result from acute infections. Considering that toxocariasis is characterized as a chronic infection, with possible reactivation of ...
Improving tuberculosis control through the partnership between university and t...
Valença,Mariana Soares; Rocha,Jeane Zanini da; Ramis,Ivy Bastos; Carrion,Lillian Lucas; Madruga,Catiúcia; Macedo,Maíra Bidart de; Scaini,Carlos JamesINTRODUCTION: Tuberculosis (TB) control is linked to the availability of qualified methods for microbiological diagnostics; however, microscopy with limited sensitivity is the only method available in many locations. The objective of this study was to evaluate the introduction of culture, drug susceptibility testing (DST), and genotyping in the routine of a Municipal Program of Tuberculosis Control. METHODS: Di...
Evaluation of diagnostic methods for the detection of Helicobacter pylori in ga...
Ramis,Ivy Bastos; Moraes,Ernani Pinho de; Fernandes,Márcia Silveira; Mendoza-Sassi,Raul; Rodrigues,Obirajara; Juliano,Carlos Renan VarelaHelicobacter pylori infects nearly 50% of the world's population. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. Currently many diagnostic methods exist for detecting H. pylori, however they all have limitations, thus it is recommend a combination of at least two methods. The aim of this study was to evaluate diag...
Contaminação ambiental por ovos e larvas de helmintos em fezes de cães na área ...
Scaini,Carlos James; Toledo,Ricardo Navarrete de; Lovatel,Raquel; Dionello,Maria Alix; Gatti,Fabiane dos Anjos; Susin,LuliePara investigar a presença de helmintos, agentes de zoonoses parasitárias, foram examinadas 237 amostras fecais de cães, na área central do principal balneário do litoral sul do Rio Grande do Sul. As principais contaminações foram: ovos e larvas do gênero Ancylostoma (71,3%), ovos de Trichuris (32,5%) e Toxocara (9,3%).