60 documents found, page 1 of 6
New quantum-dot-based fluorescent immunosensor for cancer biomarker detection
Sousa, Mariana; Piloto, Margarida; Pereira, Ana Cláudia; Schmitt, Fernando C.; Fernandes, Ruben; Moreira, FelisminaCancer antigen 15-3 (CA 15-3) is a biomarker for breast cancer used to monitor response to treatments and disease recurrence. The present work demonstrates the preparation and application of a fluorescent biosensor for ultrasensitive detection of the cancer antigen CA 15-3 protein tumor marker using mercaptopropionic-acid-functionalized cadmium telluride (CdTe; This research was funded by Fundacao para a Cienci...
Cytological Criteria for Predicting the Luminal Phenotype of Breast Carcinoma
Paschoalini, Rafael Bispo [UNESP]; Dufloth, Rozany Mucha [UNESP]; Moraes Neto, Francisco Alves; Schmitt, Fernando C.Made available in DSpace on 2018-11-26T17:12:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-09-01; Background: Specific cytological criteria for the luminal phenotype of breast carcinoma, despite it being the most common and having a better prognosis as well as targeted therapies under study, remain to be established. Using fine-needle aspiration cytology (FNAC), we aimed to identify the luminal phen...
Targeting lactate transport suppresses in vivo breast tumour growth
Morais-Santos, Filipa; Granja, Sara; Miranda-Gonçalves, Vera; Moreira, António H.J.; Queirós, Sandro; Vilaça, João L.; Schmitt, Fernando C.Background: Most cancers, including breast cancer, have high rates of glucose consumption, associated with lactate production, a process referred as “Warburg effect”. Acidification of the tumour microenvironment by lactate extrusion, performed by lactate transporters (MCTs), is associated with higher cell proliferation, migration, invasion, angiogenesis and increased cell survival. Previously, we have described...
Characterization of monocarboxylate transporters (MCTs) expression in soft tiss...
Pinheiro, Celine; Penna, Valter; Morais-Santos, Filipa; Abrahao-Machado, Lucas F.; Ribeiro, Guilherme; Curcelli, Emilio Carlos [UNESP]Made available in DSpace on 2014-12-03T13:08:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-05-09Bitstream added on 2014-12-03T13:23:21Z : No. of bitstreams: 1 WOS000336931400001.pdf: 1483231 bytes, checksum: d44026d999cd265cc444aeb241af66a4 (MD5); FCT (Portuguese Foundation for Science and Technology); FCT; Background: Soft tissue sarcomas (STSs) are a group of neoplasms, which, despite current ther...
Differential sensitivities to lactate transport inhibitors of breast cancer cel...
Santos, Filipa Morais; Gonçalves, Vera M.; Pinheiro, Sílvia; Vieira, André Filipe; Paredes, Joana; Schmitt, Fernando C.; Baltazar, FátimaThis is linked to the online version of the paper at: http://dx.doi.org/10.1530/ERC-13-0132.; The tumour microenvironment is known to be acidic due to high glycolytic rates of tumour cells. Monocarboxylate transporters (MCTs) play a role in extracellular acidification, which is widely known to be involved in tumour progression. Recently, we have described the upregulation of MCT1 in breast carcinomas and its as...
Characterization of monocarboxylate transporters (MCTs) expression in soft tiss...
Pinheiro, Céline; Penna, Valter; Santos, Filipa Morais; Abrahão-Machado, Lucas Faria; Ribeiro, Guilherme; Curcelli, Emílio C.; Olivieri, Marcus V.Background: Soft tissue sarcomas (STSs) are a group of neoplasms, which, despite current therapeutic advances, still confer a poor outcome to half of the patients. As other solid tumors, STSs exhibit high glucose consumption rates, associated with worse prognosis and therapeutic response. As highly glycolytic tumors, we hypothesized that sarcomas should present an increased expression of lactate transporters (M...
Characterization of PAR1 and FGFR1 expression in invasive breast carcinomas : p...
Tiburcio, Marta; Costa, Sandra Maria Araújo da; Duarte, Maria de Fátima Pereira; Schmitt, Fernando C.; Filho, A. L.Breast cancer is the most common cause of cancer mortality among women worldwide. Among the several factors associated with breast cancer development, angiogenesis plays an essential role and has currently emerged as a potential diag¬nostic, prognostic and therapeutic target. Protease-activated receptor 1 (PAR1) and fibroblast growth factor receptor 1 (FGFR1) have important activities in tumor angiogenesis and ...
Lactate-induced il-8 pathway in endothelial cells-letter
Pinheiro, Céline; Longatto Filho, Adhemar; Nogueira, Rosete; Schmitt, Fernando C.; Baltazar, FátimaVegran and colleagues proposed a model in which the lactate released from tumor cells through MCT4 would be taken up by endothelial cells via the MCT1 transporter and stimulate angiogenesis, using human umbilical vein endothelial cells (HUVECs) as model of tumor endothelial cells. By analyzing a total of 505 cases of human tumor samples immunostained for MCT1, we do not confirm plasma membrane expression of MCT...
Role of monocarboxylate transporters in human cancers : state of the art
Pinheiro, Céline; Longatto Filho, Adhemar; Azevedo, João Silva; Casal, Margarida; Schmitt, Fernando C.; Baltazar, FátimaMonocarboxylate transporters (MCTs) belong to the SLC16 gene family, presently composed by 14 members. MCT1-MCT4 are proton symporters, which mediate the transmembrane transport of pyruvate, lactate and ketone bodies. The role of MCTs in cell homeostasis has been characterized in detail in normal tissues, however, their role in cancer is still far from understood. Most solid tumors are known to rely on glycolys...
CD147 immunohistochemistry discriminates between reactive mesothelial cells and...
Pinheiro, Céline; Longatto Filho, Adhemar; Ramos, Tony R.; Pereira, Helena; Bedrossian, Carlos; Michael, Claire; Schmitt, Fernando C.; Baltazar, FátimaMalignant mesothelioma (MM) is a rare form of cancer. Its histopathological diagnosis is very difficult, as it exhibits a number of different appearances that can be misinterpreted as metastatic invasion or atypical hyperplasia. Thus, there is an urgent need to identify adequate markers to distinguish between benign and malignantcells, allowing the implementation of appropriate therapies and, possibly, specific...