140 documents found, page 1 of 14

Genomic and transcriptomic analysis of Saccharomyces cerevisiae isolates with f...

Succinic acid is a platform chemical that plays an important role as precursor for the synthesis of many valuable bio-based chemicals. Its microbial production from renewable resources has seen great developments, specially exploring the use of yeasts to overcome the limitations of using bacteria. The objective of the present work was to screen for succinate-producing isolates, using a yeast collection with dif...

Integrating transcriptomics and metabolomics for the analysis of the aroma prof...

Background: During must fermentation thousands of volatile aroma compounds are formed, with higher alcohols, acetate esters and ethyl esters being the main aromatic compounds contributing to floral and fruity aromas. The action of yeast, in particular Saccharomyces cerevisiae, on the must components will build the architecture of the wine flavour and its fermentation bouquet. The objective of the present work w...

Intrastrain genomic and phenotypic variability of the commercial Saccharomyces ...

The maintenance of microbial species in different environmental conditions is associated with adaptive microevolutionary changes that are shown here to occur within the descendants of the same strain in comparison with the commercial reference strain. However, scarce information is available regarding changes that occur among strain descendants during their persistence in nature. Herein we evaluate genome varia...

Combined use of Wallerstein and Zygosaccharomyces bailii modified differential ...

The level of acetic acid, the main component of volatile acidity, is critical for wine quality. Winemakers have been using a refermentation process to lower the concentration of acetic acid of wines with high volatile acidity, whichconsistsinmixingtheacidicwinewithfreshlycrushedgrapesormarcsinaproportionofnomorethan 20-30% (v/v). Though this process implies low costs it harbors the risk of unexpected and detrim...

Computational models reveal genotype-phenotype associations in Saccharomyces ce...

Genome sequencing is essential to understand individual variation and to study the mechanisms that explain relations between genotype and phenotype. The accumulated knowledge from large-scale genome sequencing projects of Saccharomyces cerevisiae isolates is being used to study the mechanisms that explain such relations. Our objective was to undertake genetic characterization of 172 S. cerevisiae strains from d...

Computational Models for Prediction of Yeast Strain Potential for Winemaking fr...

Saccharomyces cerevisiae strains from diverse natural habitats harbour a vast amount of phenotypic diversity, driven by interactions between yeast and the respective environment. In grape juice fermentations, strains are exposed to a wide array of biotic and abiotic stressors, which may lead to strain selection and generate naturally arising strain diversity. Certain phenotypes are of particular interest for th...

Pheno-metabolomics characterization of a Saccharomyces cerevisiae strain collec...

Intra-strain phenotypic and genomic variability of the commercial Saccharomyces...

The use of commercial Saccharomyces cerevisiae wine strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every harvest in large quantities. We showed that such strains are disseminated from the winery and their permanence in nature induced genetic changes, not found among a control group of isolates that derived from clonal expansion of ...

The phenotypic landscape of a Saccharomyces cerevisiae strain collection

Within our previous work [1] we developed computational models to predict strains with specific phenotypes (e.g. low ethanol resistance, growth at 30ºC and growth in media containing galactose, raffinose or urea) from microsatellite allelic patterns. The objective of the present work was to gain deeper understanding of the phenotypic diversity of a heterogeneous Saccharomyces cerevisiae strain collection, using...

Yeast biodiversity in the vineyards of the Azores Archipelago (Portugal)

This study aims to identify the fermentative yeasts communities of the vineyards from the mid-Atlantic islands ecosystems of the Azores Archipelago and to evaluate whether the populations vary according to the islands geography, grape variety and type of vineyard. From an ecological point of view, vineyards of these isolated volcanic islands terroirs correspond to very particular ecosystems, whose yeast flora i...