7 documents found, page 1 of 1
Cellulose test strips modified with virus-like particles: Advancing viral immun...
Pereira, Ana Cláudia Santos; Sousa, Cristina; Mateus, Daniela; Sebastião, Ana Isabel; Cruz, Maria Teresa; Mato, Ana Miguel; Moreira, FelisminaThe COVID-19 pandemic has driven immunity acquisition through infection or vaccination, leading to SARS-CoV-2 antibodies. This study introduces a colorimetric biosensor for COVID-19 immunity screening using Virus-Like Particles (VLPs) with SARS-CoV-2 spike protein motifs on a cellulose carrier. The cellulose was chemically modified with DSS to secure the VLPs, verified via FTIR. Detection utilized the Bradford ...
CuMV VLPs Containing the RBM from SARS-CoV-2 Spike Protein Drive Dendritic Cell...
Sebastião, Ana Isabel; Mateus, Daniela; Carrascal, Mylène A.; Sousa, Cátia; Cortes, Luísa; Bachmann, Martin F; do Carmo, Anália; Matos, Ana M.Dendritic cells (DCs) are the most specialized and proficient antigen-presenting cells. They bridge innate and adaptive immunity and display a powerful capacity to prime antigen-specific T cells. The interaction of DCs with the receptor-binding domain of the spike (S) protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a pivotal step to induce effective immunity against the S protei...
Exploring the antioxidant, anti-inflammatory and antiallergic potential of Braz...
Conte, Fernanda Lopes; Pereira, Ana Catarina; Brites, Gonçalo; Ferreira, Isabel; Silva, Ana Cristina; Sebastião, Ana Isabel; Matos, PatríciaPropolis is a bee product used since ancient times due to its diverse biological applications. We aimed to investigate the antioxidant and anti-inflammatory action of Brazilian propolis in THP-1 cells stimulated with 1‐fluoro‐2,4‐dinitrobenzene (DNFB) or lipopolysaccharide (LPS). Methods: Cell viability was assessed by resazurin assay, antioxidant activity was evaluated by DPPH assay, superoxide dismutase (SOD)...
Development of colorimetric cellulose-based test-strip for the rapid detection ...
Correia, Bárbara P.; Sousa, Mariana P.; Sousa, Cristina E. A.; Mateus, Daniela; Sebastião, Ana Isabel; Cruz, Maria Teresa; Matos, Ana MiguelGiven the pandemic situation, there is an urgent need for an accurate test to monitor antibodies anti-SARS-CoV-2, providing crucial epidemiological and clinical information to monitor the evolution of coronavirus disease in 2019 (COVID-19) and to stratify the immunized and asymptomatic population. Therefore, this paper describes a new cellulosebased test strip for rapid and cost-efective quantitative detection ...
Exploring the antioxidant, anti-inflammatory and antiallergic potential of Braz...
Conte, Fernanda Lopes [UNESP]; Pereira, Ana Catarina; Brites, Gonçalo; Ferreira, Isabel; Silva, Ana Cristina; Sebastião, Ana Isabel; Matos, PatríciaMade available in DSpace on 2022-05-01T15:13:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-05-01; Background: Propolis is a bee product used since ancient times due to its diverse biological applications. We aimed to investigate the antioxidant and anti-inflammatory action of Brazilian propolis in THP-1 cells stimulated with 1‐fluoro‐2,4‐dinitrobenzene (DNFB) or lipopolysaccharide (LPS). Methods: Ce...
Development of colorimetric cellulose-based test-strip for the rapid detection ...
Correia, Bárbara; Sousa, Mariana; Sousa, Cristina; Mateus, Daniela; Sebastião, Ana Isabel; Cruz, Maria Teresa; Matos, Ana MiguelGiven the pandemic situation, there is an urgent need for an accurate test to monitor antibodies anti-SARS-CoV-2, providing crucial epidemiological and clinical information to monitor the evolution of coronavirus disease in 2019 (COVID-19) and to stratify the immunized and asymptomatic population. Therefore, this paper describes a new cellulose-based test strip for rapid and cost-effective quantitative detectio...
NLRP3 Inflammasome and Allergic Contact Dermatitis: A Connection to Demystify
Sebastião, Ana Isabel; Ferreira, Isabel; Brites, Gonçalo; Silva, Ana; Neves, Bruno Miguel; Cruz, Maria TeresaAllergic contact dermatitis is a common occupational disease that manifests as a cell-mediated hypersensitivity reaction following skin exposure to small reactive chemicals termed haptens. Haptens penetrate the stratum corneum and covalently modify proteins in the epidermis, inducing intracellular stress, which further leads to the release of damage-associated molecular patterns (DAMPs), such as uric acid, reac...