In forested streams, leaf litter decomposition is a vital ecosystem process, governed primarily by aquatic hyphomycetes. These fungi are crucial mediators of nutrients and energy to invertebrates and higher trophic levels. Very little information is available on the impact of low concentrations of different sizes of nanoplastic particles (NPPs) on leaf litter decomposition and aquatic hyphomycetes communities. ...
The physiology, biochemistry and diversity of aquatic microbial decomposers have been largely investigated in low-order streams. However, some aspects still need further attention to better ascertain how microbial decomposer diversity can ensure ecosystem processes and services, particularly under the challenges posed by global environmental change. Aquatic microbial decomposers play a key role in processing pl...
Litter-associated microorganisms play key roles in forested streams by decomposing and transferring energy from plant litter to higher trophic levels. To circumvent the shortcomings of traditional microbiological methods and conventional microscopy to assess the composition of microbial communities associated with decomposing litter, a range of DNA-based techniques have been developed in the last 15 years. One ...
Metabarcoding of fungi and bacteria is rapidly evolving as a popular tool to assess the composition of microbial communities associated with decomposing plant litter. This development is generating vast volumes of raw data by high-throughput sequencing. One challenge in metabarcoding is to ensure the expertise to use the bioinformatics pipeline efficiently to examine the generated data. This chapter describes a...
Global patterns of biodiversity have emerged for soil microorganisms, plants and animals, and the extraordinary significance of microbial functions in ecosystems is also well established. Virtually unknown, however, are largescale patterns of microbial diversity in freshwaters, although these aquatic ecosystems are hotspots of biodiversity and biogeochemical processes. Here we report on the first large-scale st...
A principal fonte de matéria orgânica dos ribeiros de montanha são as folhas que se depositam sobre o leito maioritariamente durante o outono. Essas folhas vão passar por um processo de decomposição no qual os microorganismos aquáticos, em especial um grupo de fungos designado por hifomicetes, são os primeiros a atuar, pois conseguem quebrar moléculas complexas do material vegetal, tornando-o mais apetecível e ...
1. Climate change scenarios predict an increase in global temperature and alterations in precipitation regimes, which may change nutrient concentrations in waterbodies. In forested streams, decomposition of allochthonous organic matter is a key ecosystem process that is affected by the quality of plant litter entering the streams and several environmental factors, including nutrient concentrations, whose intera...
The water-soluble polyhydroxyfullerene (PHF) is a functionalized carbon nanomaterial with several industrial and commercial applications. There have been controversial reports on the toxicity and/or antioxidant properties of fullerenes and their derivatives. Conversely, metals have been recognized as toxic mainly due to their ability to induce oxidative stress in living organisms. We investigated the interactiv...
A decade has passed since the first DNA sequences of aquatic hyphomycete species have become available. They have illuminated aspects of their phylogeny and evolution that were inaccessible by conventional methods. Here we present examples of how the resulting information has modified our knowledge of aquatic hyphomycetes. Generating more and better DNA sequence data will continue to expand the range of questio...