57 documents found, page 1 of 6
Olive oil waste as a source of functional food ingredients: Assessing polypheno...
Ronca, Carolina L.; Marques, Sara S.; Ritieni, Alberto; Giménez-Martínez, Rafael; Barreiros, Luísa; Segundo, Marcela A.Around two million tons of olive oil are produced in Europe annually, with Portugal being among the top five European olive oil-producing countries. Olive oil production results in a substantial amount of waste in the form of olive leaves. These discarded olive leaves contain valuable phenolic compounds with antioxidant, anti-inflammatory, hypoglycaemic, neuroprotective, and antiproliferative properties. Due to...
Microcarrier-based fluorescent yeast estrogen screen assay for fast determinati...
Gregório, Bruno J.R.; Ramos, Inês I.; Marques, Sara S.; Barreiros, Luísa; Magalhães, Luís M.; Schneider, Rudolf J.; Segundo, Marcela A.The presence of endocrine-disrupting compounds (EDCs) in water poses a significant threat to human and animal health, as recognized by regulatory agencies throughout the world. The Yeast Estrogen Screen (YES) assay is an excellent method to evaluate the presence of these compounds in water due to its simplicity and capacity to assess the bioaccessible forms/fractions of these compounds. In the presence of a com...
Exploiting kinetic features of ORAC assay for evaluation of radical scavenging ...
Carvalho, Joana R. B.; Meireles, Andreia N.; Marques, Sara S.; Gregório, Bruno J. R.; Ramos, Inês I.; Silva, Eduarda M. P.; Barreiros, LuisaThe analysis and interpretation of data retrieved from Oxygen Radical Absorbance Capacity (ORAC) assays represent a challenging task. ORAC indexes originate from different mathematical approaches often lacking correct elucidation of kinetic features concerning radical scavenging reactions by antioxidant compounds. In this work, the expression of ORAC values as area under fluorescein (FL) decay curves (AUC) and ...
Sample preparation and chromatographic methods for the determination of protein...
Fernandes, Sara R.; Meireles, Andreia N.; Marques, Sara S.; Silva, Luís; Barreiros, Luisa; Sampaio-Maia, Benedita; Miro, Manuel; Segundo, Marcela A.Protein-bound uremic retention solutes, such as indole-3-acetic acid, indoxyl sulfate, p-cresol and p-cresol sulfate, are associated with the development of several pathologies, namely renal, cardiovascular, and bone toxicities, due to their potential accumulation in the human body, thus requiring analytical methods for monitoring and evaluation. The present review addresses conventional and advanced sample tre...
Lab-on-valve automated and miniaturized assessment of nanoparticle concentratio...
Marques, Sara S.; Ramos, Inês I.; Silva, Carla; Barreiros, Luisa; Domingues, Maria R.; Segundo, Marcela A.Nanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose−response correlations and for evaluating the reproduci bility of the manufacturing process. Still, faster and simpler procedures, dismissing skilled operators and post-analysis conversions are n...
Total analysis system for the determination of uremic toxins in human plasma ba...
Fernandes, Sara; Barreiros, Luísa; Sampaio-Maia, Benedita; Miró, Manuel; Segundo, Marcela A.Indoxyl sulfate (INDS) and p-cresol sulfate (pCS) are two of the most relevant uremic toxins that are recognized to have an essential role in chronic kidney disease (CKD) progression and associated cardiovascular risk. Thus, it is crucial to accurately assess their circulating levels in the body. Aiming at establishing an analytical strategy for quantification of INDS and pCS in human plasma, an automatic on-li...
Assessing the differences of two vineyards soils’ by NIR spectroscopy and chemo...
Machado, Sandia; Barreiros, Luísa; Graça, António R.; Madeira, Manuel; Páscoa, Ricardo N. M. J.; Segundo, Marcela A.; Lopes, João A.Soil properties influence greatly the status of vine plants which consequently influences the quality of wine. Therefore, in the context of viticulture management, it is extremely important to assess the physical and chemical parameters of vineyards soils. In this study, the soils of two vineyards were analysed by near-infrared (NIR) spectroscopy and established analytical reference procedures. The main objecti...
A data mining tool for untargeted biomarkers analysis: Grapes ripening application
Machado, Sandia; Barreiros, Luisa; Graça, António R.; Páscoa, Ricardo N.M.J.; Segundo, Marcela A.; Lopes, João A.In metabolomics, data generated by untargeted approaches can be very complex due to the typically extensive number of features in raw data (with and without chemical relevance), dependence on raw data preprocessing methods, and lack of selective data mining tools to appropriately interpret these data. Extraction of meaningful information from these data is still a significant challenge in metabolomics. Moreover...
Lab-on-valve automated and miniaturized assessment of nanoparticle concentratio...
Marques, Sara S.; Ramos, Inês I.; Silva, Carla; Barreiros, Luisa; Domingues, Maria R.; Segundo, Marcela A.Nanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose--response correlations and for evaluating the reproducibility of the manufacturing process. Still, faster and simpler procedures, dismissing skilled operators and post-analysis conversions are n...
Automatic and renewable micro-solid-phase extraction based on bead injection la...
Fernandes, Sara; Barreiros, Luísa; Sá, Paula; Miró, Manuel; Segundo, Marcela A.An automatic micro-solid-phase extraction (μSPE) method using on-line renewable sorbent beads followed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) was established for the determination of tranexamic acid (TXA) in urine. The μSPE method was based on the bead injection (BI) concept combined with the mesofluidic lab-on-valve (LOV) platform. All steps of the μSPE–BI–LOV were implemented by computer...