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Editorial: Fermented Foods-Characterization of the Autochthonous Microbiota

Saavedra, Maria José; Laranjo, Marta; Grujovic, Mirjana; Alves-Barroco, Cíntia; Semedo-Lemsaddek, Teresa

Editorial que introduz o Research Topic “Fermented Foods: Characterization of the Autochthonous Microbiota”, reunindo estudos sobre microbiotas autóctones em alimentos fermentados artesanais e o papel das tecnologias ómicas na compreensão da sua diversidade e função. Destaca avanços na análise de comunidades microbianas e o contributo das fermentações tradicionais para a segurança, qualidade e identidade dos al...


Antimicrobial resistance and clonal lineages of staphylococcus aureus from catt...

Silva, Vanessa; Correia, Susana; Rocha, Jaqueline; Manaia, Célia M.; Silva, Adriana; García-Díez, Juan; Pereira, José Eduardo; Semedo-Lemsaddek, Teresa

Staphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (1...


Duvan chvarci : product characterization and comparison between traditional and...

Grujovic, Mirjana; Petrovic, Tanja D. Zugic; Mladenovic, Katarina G.; Tomovic, Vladimir M.; Kocic-Tanackov, Suncica D.; Semedo-Lemsaddek, Teresa

This research aimed to investigate and compare traditional products called “duvan chvarci” produced using pork meat and fat, originated from local households and industries. Physical and chemical analysis demonstrated differences among the examined products, mostly in total chloride content and TBARS values. Samples collected at local households showed finer color (higher lightness and yellowness) and sensory p...


Serbian traditional goat cheese : physico-chemical, sensory, hygienic and safet...

Mladenovic, Katarina G.; Grujovic, Mirjana Z.; Kocic-Tanackov, Suncica D.; Bulut, Sandra; Ilicic, Mirela; Degenek, Jovana; Semedo-Lemsaddek, Teresa

This research project aimed to investigate the physico-chemical, sensory, hygienic and safety characteristics of raw goat milk, whey, brine and traditional goat cheese during the ripening period of 28 days. Physico-chemical parameters included the determination of dry matter, fat, ash, protein, pH, water activity and NaCl content. The presence of Enterobacteriaceae and fungi was estimated on milk and cheese sam...


Enterococcus spp. from Azeitao and Nisa PDO-cheeses: surveillance for antimicro...

Rocha, Patrícia A. Bastiao; Monteiro Marques, Joana; BARRETO, ANTONIO; Semedo-Lemsaddek, Teresa

ABSTRACT - Enterococcus spp. were isolated from PDO-cheese of Azeitão and Nisa at six cheesemaking units (Azeitão: A1, A2, A3, A4; and Nisa: N9, N10), over four years (2016–2019). Genomic typing was performed using RAPD and distinct enterococci (n = 145) were identified at the species level by multiplex-PCR and evaluated regarding antimicrobial drug resistance (AMR). Antibiotics from nine distinct classes (amin...


Advantages and disadvantages of non-starter lactic acid bacteria from tradition...

Grujovic, Mirjana Z.; Mladenovic, Katarina G.; Semedo-Lemsaddek, Teresa; Laranjo, Marta; Stefanovic, Olgica D.; Kocic-Tanackov, Suncica D.

Traditional fermented foods are a significant source of starter and/or non-starter lactic acid bacteria (nsLAB). Moreover, these microorganisms are also known for their role as probiotics. The potential of nsLAB is huge; however, there are still challenges to be overcome with respect to characterization and application. In the present review, the most important steps that autochthonous lactic acid bacteria isol...


Resistance and virulence distribution in enterococci isolated from broilers rea...

Semedo-Lemsaddek, Teresa; Cota, João; Ribeiro, Tânia; Pimentel, Amélia; Tavares, Luis; Bernardo, Fernando; Oliveira, Manuela

Background: The impact of enterococci in human health has been growing for the last decades, mainly due to their resistance to several antimicrobial agents. Human consumption of contaminated meat, especially poultry, has been identified as a possible route of transmission. The aim of the present study was to evaluate and compare the antimicrobial resistance profiles and virulence genes of enterococci isolated f...


Antimicrobial effects of essential oils on oral microbiota biofilms: the toothb...

Aires, Andreia; BARRETO, ANTONIO; Semedo-Lemsaddek, Teresa

The present investigation intended to evaluate the bacteriostatic and bactericidal abilities of clove, oregano and thyme essential oils against oral bacteria in planktonic and biofilm states. Furthermore, aiming to mimic everyday conditions, a toothbrush in vitro model was developed. Determination of the minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentrati...


Characterization and Technological Features of Autochthonous Coagulase-Negative...

Semedo-Lemsaddek, Teresa; Carvalho, Laura; Tempera, Carolina; Fernandes, Maria H.; Fernandes, Maria J.; Elias, Miguel; Barreto, António S.

The manufacture of dry fermented sausages is an important part of the meat industry in Southern Europeancountries. These products are usually produced in small shops from a mixture of pork, fat, salt, and condiments andare stuffed into natural casings. Meat sausages are slowly cured through spontaneous fermentation by autochthonousmicrobiota present in the raw materials or introduced during manufacturing. The a...


Characterization of multidrug-resistant diabetic foot ulcer enterococci

Semedo-Lemsaddek, Teresa; Mottola, Carla; Alves-Barroco, Cynthia; Cavaco-Silva, Patrícia; Tavares, Luís; Oliveira, Manuela

Introduction: Diabetes mellitus is a highly prevalent chronic progressive disease with complications that include diabetic-foot ulcers. Methods: Enterococci isolated from diabetic-foot infections were identified, evaluated by macrorestriction analysis, and screened for virulence traits and antimicrobial resistance. Results: All isolates were considered multidrug-resistant, cytolysin and gelatinase producers, an...


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