6 documents found, page 1 of 1
Interactions of “de novo” designed peptides with bacterial membranes: implicati...
Maturana, Patricia; Gonçalves, Sónia; Martinez, Melina; Espeche, Juan C.; Santos, Nuno C.; Semorile, Liliana; Maffia, Paulo C.; Hollmann, AxelAntimicrobial peptides are small molecules that display antimicrobial activity against a wide range of pathogens. In a previous work, by using model membranes we studied P6, a peptide that shows no antimicrobial activity, and P6.2, which exhibits antibacterial activity. In the present work we aimed to unravel the mode of action of these peptides by studying their interaction in vivo with Escherichia coli and St...
Synergistic and antibiofilm activity of the antimicrobial peptide P5 against ca...
Martinez, Melina; Gonçalves, Sónia; Felício, Mário Romão; Maturana, Patricia; Santos, Nuno C.; Semorile, Liliana; Hollmann, Axel; Maffía, Paulo C.In the search for new antimicrobial molecules, antimicrobial peptides (AMPs) offer a viable alternative to conventional antibiotics, as they physically disrupt the bacterial membranes, leading to membrane disruption and eventually cell death. In particular, the group of linear α-helical cationic peptides has attracted increasing research and clinical interest. The AMP P5 has been previously designed as a cation...
Interaction of S-layer proteins of Lactobacillus kefir with model membranes and...
Hollmann, Axel; Delfederico, Lucrecia; Santos, Nuno C.; Disalvo, E. Anibal; Semorile, LilianaIn previous works, it was shown that S-layer proteins from Lactobacillus kefir were able to recrystallize and stabilize liposomes, this feature reveling a great potential for developing liposomal-based carriers. Despite previous studies on this subject are important milestones, a number of questions remain unanswered. In this context, the feasibility of S-layer proteins as a biomaterial for drug delivery was ev...
Cell surface damage and morphological changes in Oenococcus oeni after freeze-d...
Bravo-Ferrada, Bárbara Mercedes; Abreu, Sónia Gonçalves; Semorile, Liliana; Santos, Nuno C.; Brizuela, Natalia S.; Elizabeth Tymczyszyn, E.The aim of the present study was to evaluate the effects of freeze-drying in the presence of trehalose as a cryoprotectant, followed by incubation in synthetic wine, on surface damage, viability and l-malic acid consumption of the oenological strain Oenococcus oeni UNQOe 73.2. After freeze-drying, no significant differences were observed in the number of viable cells (for both acclimated and non-acclimated cult...
Role of amphipathicity and hydrophobicity in the balance between hemolysis and ...
Hollmann, Axel; Martínez, Melina; Noguera, Martín E.; Augusto, Marcelo T.; Disalvo, Anibal; Santos, Nuno C.; Semorile, Liliana; Maffía, Paulo C.Cationic antimicrobial peptides (CAMPs) represent important self defense molecules in many organisms, including humans. These peptides have a broad spectrum of activities, killing or neutralizing many Gram-negative and Gram-positive bacteria. The emergence of multidrug resistant microbes has stimulated research on the development of alternative antibiotics. In the search for new antibiotics, cationic antimicrob...
Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lacto...
Hollmann, Axel; Saviello, Mariano; Delfederico, Lucrecia; Saraiva, Tessália Diniz Luerce; Barh, Debmalya; Jain, Neha; Tiwari, Sandeep; Chandra, SudhaProkaryotes commonly present outer cell wall structures composed of a crystalline array of proteinaceous subunits, known as surface layers (S-layers). The ORF encoding the S-layer protein (SlpA) of Lactobacillus brevis was cloned into Lactococcus lactis under the transcriptional control of the xyloseinducible expression system (XIES). SlpA was secreted into the extracellular medium, as determined by immunoblott...