103 documents found, page 1 of 11
Design of polymeric core-shell carriers for combination therapies
Silva, Dina M.; Liu, Ruy; Gonçalves, Anabela F.; da Costa, André; Gomes, Andreia C; Machado, Raúl; Vongsvivut, Jitraporn; Tobin, Mark J.Particle engineering for co-delivery of drugs has the potential to combine multiple drugs with different pharmaceutical mechanisms within the same carrier, increasing the therapeutic efficiency while improving patient compliance. This work proposes a novel approach for producing polymer–polymer core–shell microparticles by multi-step processing of emulsion and spray drying. The particle core was obtained by an ...
Genetically engineered protein-based polymers with broad antimicrobial activity...
da Costa, André; Sencadas, Vítor João Gomes Silva; Lanceros-Méndez, S.; Gomes, Andreia C; Rodriguez-Cabello, Jose; Casal, Margarida; Machado, RaulWith increasing healthcare-associated infections and antibiotic-resistant microorganisms there is a demand not only for new antimicrobial compounds but also for antimicrobial materials. With the use of synthetic protein biotechnology approaches and recombinant DNA technology, we can now create new tailor-made materials with precise control over its sequence. Indeed, by combining antimicrobial activity of natura...
Genetically engineered Silk-Elastin-Like Proteins as a versatile platform for t...
Machado, Raul; Costa, André Manuel Abreu; Pereira, Ana Margarida Macedo Bernardes; Silva, Andreia Maria; Gomes, Diana; Gomes, Andreia C[Excerpt] Throughout evolution nature created and refined proteins for a wide range of functions, working as structural components or as molecular motors. In this sense, the natural fibrous proteins represent the utmost case of function specialization and high performance materials. The remarkable mechanical properties of proteins like elastin or silk are founded on conservative blocks of amino acid sequences t...
Electrospun silk-elastin fibres functionalized with silver nanoparticles as ant...
Costa, André Manuel Abreu; Silva, Andreia Maria; Pereira, Ana Margarida Macedo Bernardes; Gomes, Andreia C; Sencadas, Vítor João Gomes Silva[Excerpt] Silk-elastin-like proteins (SELPs) are a class of bioinspired, genetically engineered block copolymers, composed of silk and elastin repeating units. As base materials for biomedical purposes, SELP nanofibre mats demonstrate potential to be applied as wound dressing materials [1]. The increasing antimicrobial resistance associated with the excessive and inappropriate use of antibiotics demands the res...
Single step fabrication of antimicrobial fibre mats from a bioengineered protei...
Costa, André Manuel Abreu; Pereira, Ana Margarida Macedo Bernardes; Gomes, Andreia C; Rodríguez-Cabello, José Carlos; Sencadas, Vítor João Gomes SilvaGenetically engineered protein polymers functionalized with bioactive domains have potential as multifunctional versatile materials for biomedical use. The present work describes the fabrication and characterisation of antimicrobial fibre mats comprising the antimicrobial elastin-like recombinamer (ELR) CM4-A200. The CM4-A200 protein polymer derives from the genetic fusion of the ABP-CM4 antimicrobial peptide f...
Antibacterial protein-based fibres: combining recombinant DNA technology with e...
Costa, André Manuel Abreu; Sencadas, Vítor João Gomes Silva; Lanceros-Méndez, S.; Gomes, Andreia C; Rodríguez-Cabello, J.C.; Casal, Margarida[Excerpt] With the increasing healthcare-associated infections and antibiotic-resistant microorganisms there is a demand not only for new antimicrobial compounds but also for antimicrobial materials. Genetically engineered protein polymers functionalized with bioactive domains offer potential as multifunctional versatile materials for biomedical use. The present work describes the fabrication and characterizati...
Advanced techniques for characterizing bioinspired materials
Machado, Raul; Sencadas, Vítor João Gomes SilvaUnderstanding materials’ analytical techniques is a key feature to ensure efficient material development and to fully evaluate their functional properties. Biobased materials are emergent materials that are attracting attention due to their outstanding properties, pushing forward their use in a wide range of applications going from biomedical and pharmacy to even food industry, among others. This chapter provid...
Development of poly(vinylidene fluoride)/ionic liquid electrospun fibers for ti...
Dias, Juliana C.; Correia, D. C.; Lopes, Ana C.; Ribeiro, Sylvie Oliveira; Ribeiro, Clarisse; Sencadas, Vítor João Gomes Silva; Botelho, GabrielaElectroactive electrospun fiber mat composites based on poly (vinylidene fluoride) (PVDF) with 5 and 10% of 1-ethyl-3-methylimidazolium bis (trifluoromethanesulfonyl)amide ([C2mim][NTf2]) ionic liquid (IL) were developed for potential applications in the biomedical area. The morphology and polymer crystalline phase content of the fibers were evaluated as a function of the processing conditions. Hydrophobic rand...
Mechanical fatigue performance of PCL-chondroprogenitor constructs after cell c...
Panadero, J. A.; Sencadas, Vítor João Gomes Silva; Silva, S. C. M.; Ribeiro, C.; Correia, V.; Gama, F. M.; Gomez Ribelles, J. L.; Lanceros-Méndez, S.In tissue engineering of cartilage, polymeric scaffolds are implanted in the damaged tissue and subjected to repeated compression loading cycles. The possibility of failure due to mechanical fatigue has not been properly addressed in these scaffolds. Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. This is related to inherent discontinuities in the materi...
TiO2/graphene oxide immobilized in P(VDF-TrFE) electrospun membranes with enhan...
Almeida, Nuno A.; Martins, Pedro Manuel Abreu; Teixeira, Sara; Lopes da Silva, José A.; Sencadas, Vítor João Gomes Silva; Kühn, K.; Cuniberti, G.Here, we report on the electrospinning of poly(vinylidene difluoride-co-trifluoroethylene) (P(VDF-TrFE)) copolymer fibrous membranes decorated with titanium dioxide/graphene oxide (TiO2/GO). The presence of the TiO2/GO increases the photocatalytic efficiency of the nanocomposite membrane towards the degradation of methylene blue (MB) when compared with the membranes prepared with naked TiO2, in UV and particula...