14 documents found, page 1 of 2
Analysis of Antibody Data Using Skew-normal and Skew-T Mixture Models
Dias Domingues , Tiago; Mouriño, Helena; Sepúlveda, NunoGaussian mixture models, which assume a Normal distribution for each component, are popular in antibody (or serological) data analysis to help determining antibody-positive and antibody-negative individuals. In this work, we advocate using finite mixture models based on Skew-Normal and Skew-t distributions for serological data analysis. These flexible mixing distributions have the advantage of describing right ...
Post-Infection Fatigue Syndromes in the Era of Long COVID: The Case of Myalgic ...
Sepúlveda, Nuno; Malato, João; Winck, João Carlos; Vaz Carneiro, António; Serra Hoffman, Joan; Branco, JaimeN/A; N/A
IgG Antibody responses to Epstein-Barr Virus in Myalgic Encephalomyelitis/Chron...
Fonseca, André; Szysz, Mateusz; Ly, Hoang Thien; Cordeiro, Clara; Sepúlveda, NunoThe diagnosis and pathology of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) remain under debate. However, there is a growing body of evidence for an autoimmune component in ME/CFS caused by the Epstein-Barr virus (EBV) and other viral infections. Materials and Methods: In this work, we analyzed a large public dataset on the IgG antibodies to 3054 EBV peptides to understand whether these immune re...
Antibody selection strategies and their impact in predicting clinical malaria b...
Fonseca, André; Spytek, Mikolaj; Biecek, Przemysław; Cordeiro, Clara; Sepúlveda, NunoNowadays, the chance of discovering the best antibody candidates for predicting clinical malaria has notably increased due to the availability of multi-sera data. The analysis of these data is typically divided into a feature selection phase followed by a predictive one where several models are constructed for predicting the outcome of interest. A key question in the analysis is to determine which antibodies sh...
Revisiting IgG antibody reactivity to epstein-barr virus in myalgic encephalomy...
Sepúlveda, Nuno; Malato, João; Sotzny, Franziska; Grabowska, Anna D.; Fonseca, André; Cordeiro, Clara; Graça, Luís; Biecek, Przemyslaw; Behrends, UtaInfections by the Epstein-Barr virus (EBV) are often at the disease onset of patients suffering from Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS). However, serological analyses of these infections remain inconclusive when comparing patients with healthy controls (HCs). In particular, it is unclear if certain EBV-derived antigens eliciting antibody responses have a biomarker potential for disease ...
The SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) in myalgic encep...
Malato, João; Sotzny, Franziska; Bauer, Sandra; Freitag, Helma; Fonseca, André; Grabowska, Anna D.; Graça, Luís; Cordeiro, Clara; Nacul, LuísPeople with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) often report a high frequency of viral infections and flu-like symptoms during their disease course. Given that this reporting agrees with different immunological abnormalities and altered gene expression profiles observed in the disease, we aimed at answering whether the expression of the human angiotensin-converting enzyme 2 (ACE2), the m...
Relevance of feline interferon omega for clinical improvement and reduction of ...
Gil, Solange; Leal, Rodolfo O.; Duarte, Ana; McGahie, David; Sepúlveda, Nuno; Siborro, Inês; Cravo, Joana; Cartaxeiro, Clara; Tavares, Luís M.Feline Immnunodeficiency (FIV) and Feline Leukemia (FeLV) viruses are common infectious agents in stray cats and shelter environments. Recombinant feline interferon-x (rFeIFNx) has shown an antiviral action not only against FIV and FeLV but also against herpesvirus (FHV-1) and calicivirus (FCV). Sixteen naturally infected FIV/FeLV cats were followed during rFeIFNx therapy in order to monitor clinical signs and ...
Highly dynamic host actin reorganization around developing Plasmodium inside he...
Gomes-Santos, Carina S. S.; Itoe, Maurice A.; Afonso, Cristina; Henriques, Ricardo; Gardner, Rui; Sepúlveda, Nuno; Simões, Pedro D.; Raquel, HelenaPlasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside a parasitophorous vacuole. The nature of the Plasmodium-host cell interface, as well as the interactions occurring between these two organisms, remains largely unknown. Here we show that highly dynamic hepatocyte actin reorganization events occur around ...
Highly dynamic host actin reorganization around developing Plasmodium inside he...
Gomes-Santos, Carina S S; Itoe, Maurice A; Afonso, Cristina; Henriques, Ricardo; Gardner, Rui; Sepúlveda, Nuno; Simões, Pedro D; Raquel, HelenaPlasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside a parasitophorous vacuole. The nature of the Plasmodium-host cell interface, as well as the interactions occurring between these two organisms, remains largely unknown. Here we show that highly dynamic hepatocyte actin reorganization events occur around ...
Differentiation of human thymic regulatory T cells at the double positive stage
Nunes-Cabaço, Helena; Caramalho, Íris; Sepúlveda, Nuno; Sousa, Ana E.Treg cells, best identified by the expression of the transcription factor FOXP3, play a crucial role in maintaining self-tolerance. Natural Treg cells constitute an independent thymus-derived T-cell lineage whose developmental program in humans is still ill-defined. Here, we provide evidence of a Treg-cell differentiation pathway at the double positive (DP) stage, prior to commitment to the CD4(+) or CD8(+) lin...