2 documents found, page 1 of 1
Short term culture with cAMP modulators before vitrification significantly impr...
Monteiro, Clara Ana Santos; Leal, Gabriela Ramos; Saraiva, Helena Fabiana Reis de Almeida; Garcia, Joaquim Mansano [UNESP]Made available in DSpace on 2018-12-11T17:31:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-03-01; Oocyte cryopreservation is a strategic tool for assisted reproduction, but has limited use due to the complex cellular structure of oocytes, which leads to sub-optimal survival rates. In this study, we used the SPOM in vitro maturation system, which is based on supplementation of cAMP modulators in orde...
Developmental competence and expression of the MATER and ZAR1 genes in immature...
Mota, Gustavo Bruno; Batista, Ribrio Ivan Tavares Pereira; Serapião, Raquel Varella; Boité, Mariana Cortes; Viana, João Henrique MoreiraThe objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BC...