4 documents found, page 1 of 1
The biological potential hidden in inclusion bodies
Gifre-Renom, L; Seras-Franzoso, J; Rafael, D; Andrade, F; Cano-Garrido, O; Martinez-Trucharte, F; Ugarte-Berzal, E; Martens, E; Boon, L; Villaverde, AInclusion bodies (IBs) are protein nanoclusters obtained during recombinant protein production processes, and several studies have demonstrated their potential as biomaterials for therapeutic protein delivery. Nevertheless, IBs have been, so far, exclusively sifted by their biological activity in vitro to be considered in further protein-based treatments in vivo. Matrix metalloproteinase-9 (MMP-9) protein, whic...
Intracellular delivery of anti-SMC2 antibodies against cancer stem cells
Montero, S; Seras-Franzoso, J; Andrade, F; Martinez-Trucharte, F; Vilar-Hernández, M; Quesada, M; Xandri, H; Arango, D; Abasolo, I; Rafael, DStructural maintenance of chromosomes protein 2 (SMC2) is a central component of the condensin complex involved in DNA supercoiling, an essential process for embryonic stem cell survival. SMC2 over-expression has been related with tumorigenesis and cancer malignancy and its inhibition is regarded as a potential therapeutic strategy even though no drugs are currently available. Here, we propose to inhibit SMC2 b...
Sterilization procedure for temperature-sensitive hydrogels loaded with silver ...
Rafael, D; Andrade, F; Martinez-Trucharte, F; Basas, J; Seras-Franzoso, J; Palau, M; Gomis, X; Pérez-Burgos, M; Blanco, A; López-Fernández, A; Vélez, RHydrogels (HG) have recognized benefits as drug delivery platforms for biomedical applications. Their high sensitivity to sterilization processes is however one of the greatest challenges regarding their clinical translation. Concerning infection diseases, prevention of post-operatory related infections is crucial to ensure appropriate patient recovery and good clinical outcomes. Silver nanoparticles (AgNPs) ha...
Dynamism, sensitivity, and consequences of mesenchymal and stem-like phenotype ...
Gener, P; Seras-Franzoso, J; Callejo, P; Andrade, F; Rafael, D; Martínez, F; Montero, S; Arango, D; Sayós, J; Abasolo, I; Schwartz, SThere are remarkable similarities in the description of cancer stem cells (CSCs) and cancer cells with mesenchymal phenotype. Both cell types are highly tumorigenic, resistant against common anticancer treatment, and thought to cause metastatic growth. Moreover, cancer cells are able to switch between CSC and non-CSC phenotypes and vice versa, to ensure the necessary balance within the tumor. Likewise, cancer c...