6 documents found, page 1 of 1
m6A landscape is more pervasive when Trypanosoma brucei exits the cell cycle
Serra, Lúcia; Pereira, Sara Silva; Viegas, Idálio J.; Machado, Henrique; López-Escobar, Lara; Figueiredo, Luisa M.N6-methyladenosine (m6A) is an mRNA modification with important roles in gene expression. In African trypanosomes, this post-transcriptional modification is detected in hundreds of transcripts, and it affects the stability of the variant surface glycoprotein (VSG) transcript in the proliferating blood stream form. However, how the m6A landscape varies across the life cycle remains poorly defined. Using full-len...
Validity and reliability of a new incremental step test for people with chronic...
Vilarinho, Rui; Serra, Lúcia; Águas, Ana; Alves, Carlos; Silva, Pedro Matos; Caneiras, Cátia; Mesquita Montes, AntónioIncremental step tests (IST) can be used to assess exercise capacity in people with chronic obstructive pulmonary disease (COPD). The development of a new step test based on the characteristics of the incremental shuttle walk test (ISWT) is an important study to explore. We aimed to develop a new IST based on the ISWT in people with COPD, and assess its validity (construct validity) and reliability, according t...
Validity and reliability of a new incremental step test for people with chronic...
Vilarinho, Rui; Serra, Lúcia; Águas, Ana; Alves, Carlos; Silva, Pedro Matos; Caneiras, Catia; Montes, António MesquitaBackground: Incremental step tests (IST) can be used to assess exercise capacity in people with chronic obstructive pulmonary disease (COPD). The development of a new step test based on the characteristics of the incremental shuttle walk test (ISWT) is an important study to explore. We aimed to develop a new IST based on the ISWT in people with COPD, and assess its validity (construct validity) and reliability,...
N6-methyladenosine in poly(A) tails stabilize VSG transcripts
Viegas, Idálio; Macedo, Juan; Serra, Lúcia; De Niz, Mariana; Temporão, Adriana; Silva Pereira, Sara; Mirza, Aashiq H.; Bergstrom, Ed; Rodrigues, Joao A.RNA modifications are important regulators of gene expression1. In Trypanosoma brucei, transcription is polycistronic and thus most regulation happens post-transcriptionally2. N6-methyladenosine (m6A) has been detected in this parasite, but its function remains unknown3. Here we found that m6A is enriched in 342 transcripts using RNA immunoprecipitation, with an enrichment in transcripts encoding variant surfac...
Effects of a home-based pulmonary rehabilitation program in patients with Chron...
Vilarinho, Rui; Serra, Lúcia; Coxo, Ricardo; Carvalho, João; Esteves, Cátia; Montes, António Mesquita; Caneiras, Cátia; Vilarinho, RuiPatients with chronic obstructive pulmonary disease (COPD) in the Global Initiative for Chronic Obstructive Lung Disease (GOLD) B group can be included in pulmonary rehabilitation (PR) settings outside the hospitals. This study aimed to explore the feasibility of a home-based pulmonary rehabilitation (HBPR) program and assess its impact on patients with COPD in the GOLD B group. A real-world, pre–post intervent...
Effects of a home-based pulmonary rehabilitation program in patients with chron...
Vilarinho, Rui; Serra, Lúcia; Coxo, Ricardo; Carvalho, João; Esteves, Cátia; Montes, António Mesquita; Caneiras, CatiaPatients with chronic obstructive pulmonary disease (COPD) in the Global Initiative for Chronic Obstructive Lung Disease (GOLD) B group can be included in pulmonary rehabilitation (PR) settings outside the hospitals. This study aimed to explore the feasibility of a home-based pulmonary rehabilitation (HBPR) program and assess its impact on patients with COPD in the GOLD B group. A real-world, pre-post intervent...