2 documents found, page 1 of 1
Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis b...
Silva, Joas Lucas da; Leite, Gabriela Guimaraes Sousa; Bastos, Gisele Medeiros; Lucas, Beatriz Cacciacarro; Shinohara, Daniel KenitiSubmitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:45:39Z No. of bitstreams: 1 S0074-02762013000100017.pdf: 593309 bytes, checksum: 15f368129674a2e309ff079c8cd1c297 (MD5); Made available in DSpace on 2013-08-22T18:45:39Z (GMT). No. of bitstreams: 1 S0074-02762013000100017.pdf: 593309 bytes, checksum: 15f368129674a2e309ff079c8cd1c297 (MD5) Previous issue date: 2013-02-01; Made availabl...
Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis b...
Silva, Joas Lucas da; Leite, Gabriela Guimaraes Sousa; Bastos, Gisele Medeiros; Lucas, Beatriz Cacciacarro; Shinohara, Daniel KenitiQuantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis was used to screen for mutations related to drug resistance in Mycobacterium tuberculosis. We detected the C526T and C531T mutations in the rifampicin resistance-determining region (RRDR) of the rpoB gene with qPCR-HRM using plasmid-based controls. A segment of the RRDR region from M. tuberculosis H37Rv and from strains carrying ...