Simultaneous identification and association of body fluids to donors can serve as a powerful tool in the criminal investigation of mixed traces. Massively parallel sequencing of mRNA targets not only identifies the origin of the body fluids but may also provide additional contextual information about the body fluid donors of a (binary) mixture using coding region SNPs (cSNPs). Within the European DNA Profiling ...
The European DNA Profiling Group (EDNAP) has previously evaluated the performance, robustness, and reproducibility of various mRNA markers for identifying body fluids using capillary electrophoresis (CE) and massively parallel sequencing (MPS) methods. MPS of mRNA targets is used for body fluid identification and provides information on who contributed which body fluid to a binary mixture, thereby adding import...