35 documents found, page 1 of 4
Valorisation of olive oil by-products into pectic- and glucuronoxylo-oligosacch...
Cordeiro, Ana; Fernandes, Andreia; Ferreira, Andreia S.; Coelho, Elisabete; Coimbra, Manuel A.; Silvério, Sara C.; Cadavez, Vasco; Peres, António M.Olive pomace (OLP) and stones (OLS) are key by-products of olive oil production, rich in lignocellulose and pectin, making them viable substrates for prebiotic oligosaccharide (OS) production. This study evaluated the chemical composition of OLP and OLS powders (OLPp and OLSp) and their potential for OS production through one-step fermentation using recombinant Bacillus subtilis 3610. Both substrates had compar...
Integrated strategy for purification of esterase from Aureobasidium pullulans
Lemes, A.; Silvério, Sara C.; Rodrigues, Sueli; Rodrigues, L. R.Esterases catalyze the cleavage and formation of ester bonds of a broad range of substrates presenting a widespread spectrum of industrial applications. This work aimed to partially purify and characterize an esterase from Aureobasidium pullulans LABIOTEC 01 produced in a culture medium containing olive mill wastewater. Esterase purification was evaluated using different strategies, namely the enzyme recovery b...
One-step process for producing prebiotic arabino-xylooligosaccharides from Brew...
Amorim, Cláudia; Silvério, Sara C.; Rodrigues, L. R.Xylooligosaccharides (XOS) are prebiotic nutraceuticals that can be sourced from lignocellulosic biomass, such as agro-residues. This study reports for the first time an optimization study of XOS production from agro-residues by direct fermentation using two Trichoderma species. A total of 13 residues were evaluated as potential substrates for single-step production. The best results were found for Trichoderma ...
Evaluation of the prebiotic galacto-oligosaccharides addition in the traditiona...
Moreira, Ramon; Amorim, Cláudia; Cardoso, B.; Silvério, Sara C.; Rodrigues, L. R.The global epidemic of overweight and obesity have become a major public health problem in several parts of the world. The increasing prevalence of those medical conditions is associated with many diet-related chronic diseases (diabetes mellitus, cardiovascular disease, etc.). The consumers prefer low-sugar and functional food products. In this context, prebiotics compounds have attracted global attention as fu...
Downscale fermentation for xylooligosaccharides production by recombinant Bacil...
Amorim, Cláudia; Silvério, Sara C.; Gonçalves, Raquel F. S.; Pinheiro, Ana Cristina; Silva, Soraia; Coelho, Elisabete; Coimbra, Manuel A.The global demand of prebiotics such as xylooligosaccharides (XOS) has been growing over the years, motivating the search for different production processes with increased efficiency. In this study, a cloned Bacillus subtilis 3610, containing the xylanase gene xyn2 of Trichoderma reesei coupled with an endogenous secretion tag, was selected for XOS production through direct fermentation of beechwood xylan. A mi...
Integrated bioprocess approach for the production of xylooligosaccharides
Amorim, Cláudia; Silvério, Sara C.; Rodrigues, L. R.The demand of prebiotic ingredients has been growing over the years as consumers pay more attention to their health. Xylooligosaccharides (XOS) are considered emergent and competitively priced prebiotics, presenting high potential as food ingredients. As a result, the industry is focused on developing new approaches to improve their production efficiency to meet the increasing demand while reducing costs. Hence...
Single-step production of arabino-xylooligosaccharides by recombinant Bacillus ...
Amorim, Cláudia; Silvério, Sara C.; Silva, Soraia P.; Coelho, Elisabete; Coimbra, Manuel A.; Prather, Kristala L. J.; Rodrigues, Lígia R.Brewers' spent grain (BSG) is an inexpensive and abundant brewery by-product that can be used to produce prebiotic arabino-xylooligosaccharides (AXOS). In this study, Bacillus subtilis 3610 was used, for the first time, to produce AXOS through direct fermentation of BSG. Additionally, the microorganism was genetically modified to improve the AXOS production. The xylanase gene xyn2 from Trichoderma reesei couple...
Purification and characterization of a collagenase from Penicillium sp. UCP 128...
Wanderley, M.; Neto, José Manoel Wanderley Duarte; Albuquerque, Wendell Wagner Campos; Marques, Daniela de Araújo Viana; Lima, Carolina de AlbuquerqueCollagenases are proteolytic enzymes capable of degrading both native and denatured collagen, reported to be applied in industrial, medical and biotechnological sectors. Liquid-liquid extraction using aqueous two-phase system (ATPS) is one of the most promising bioseparation techniques, which can substitute difficult solid-liquid separation processes, offering many advantages over conventional methods including...
Trypsin purification using magnetic particles of azocasein-iron composite
Alves, Maria Helena; Nascimento, Gabriela Ayres; Cabrera, Mariana Paola; Silvério, Sara C.; Gonçalves, Clarisse Salomé Nobre; Teixeira, J. A.This work presents an inexpensive, simple and fast procedure to purify trypsin based on affinity binding with ferromagnetic particles of azocasein composite (mAzo). Crude extract was obtained from intestines of fish Nile tilapia (Oreochromis niloticus) homogenized in buffer (01 g tissue/ml). This extract was exposed to 100mg of mAzo and washed to remove unbound proteins by magnetic field. Trypsin was leached of...
ß-galactosidase from Aspergillus lacticoffeatus: A promising biocatalyst for th...
Cardoso, B.; Silvério, Sara C.; Abrunhosa, Luís; Teixeira, J. A.; Rodrigues, L. R.ß-galactosidase (EC 3.2.1.23) are interesting enzymes able to catalyze lactose hydrolysis and transfer reactions to produce lactose-based prebiotics with potential application in the pharmaceutical and food industry. In this work, Aspergillus lacticoffeatus is described, for the first time, as an effective -galactosidase producer. The extracellular enzyme production was evaluated in synthetic and alternative me...