82 documents found, page 1 of 9
Modulation of enzymatic activity by moderate electric fields: perspectives for ...
Albuquerque, Tiago; Pereira, Ricardo Nuno Correia; Silvério, Sara Isabel Cruz; Rodrigues, L. R.Modulating enzymatic activity through physical strategies is increasingly recognized as a powerful approach to optimizing biocatalytic processes in food and biotechnology applications. Cellobiose 2-epimerase (C2E), a key enzyme for synthesizing epilactose, a non-digestible disaccharide with established prebiotic effects, is gaining relevance in functional foods. Emerging strategies, such as the application of m...
Valorisation of olive oil by-products into pectic- and glucuronoxylo-oligosacch...
Cordeiro, Ana; Fernandes, Andreia; Silvério, Sara Isabel Cruz; Coelho, Elisabete; Coimbra, Manuel A.; António M. Peres; Rodrigues, L. R.Olive pomace (OLP) and stones (OLS) are key by-products of olive oil production, rich in lignocellulose and pectin, making them viable substrates for prebiotic oligosaccharide (OS) production. This study evaluated the chemical composition of OLP and OLS powders (OLPp and OLSp) and their potential for OS production through one-step fermentation using recombinant Bacillus subtilis 3610. Both substrates had compar...
Biotechnological production of natural pigments for textile dyeing
Basto, B.; Silva, Nuno Miguel Sampaio Ribeiro Magalhães; Teixeira, J. A.; Silvério, Sara Isabel CruzGlobal markets, including the pigments sector, have been significantly influenced by the adoption of novel circular bioeconomy policies and increasing concerns about sustainable practices. The natural pigments market is expected to grow considerably due to policies promoting renewable resources and reducing environmental impact. Natural pigments, particularly fungi-derived ones, are increasingly favored for the...
Fodinibius alkaliphilus sp. nov., a moderately halophilic and alkaliphilic bact...
He, Yang; Simões, Marta Filipa Jesus Freitas; de la Haba, Rafael R.; Santos-Pereira, Cátia; Pereira-Sousa, Joana; Gomes, Joana S.A novel moderately halophilic and alkaliphilic Gram-stain-negative, strictly aerobic, bacterial strain (N2T) was isolated from an inland saltern in central Portugal. The taxonomic position of this isolate was determined based on polyphasic taxonomic and phylogenomic analysis. Phylogenetic analysis based on 16S rRNA gene sequences indicated that isolate N2T belongs to the genus Fodinibius, showing the highest si...
Use of ice cream containing probiotic an effective way to control dental caries?
Sette-de-Souza, Pedro Henrique; Amorim, Rebeca de Siqueira; Cartaxo, Rebeca de Oliveira; Silvério, Sara Isabel Cruz; Lima, L.To perform a systematic review to assess the decrease in bacterial counts of Streptococcus mutans in patients who used ice cream enriched with probiotics. Is registered in the PROSPERO platform, by the code CRD42022371298. The databases were Scopus, Web of Science, Embase, Pubmed, Medline, and Science Direct, through a search algorithm based on the following PICO question: P-individuals systematically healthy a...
Manufacture of food ingredients with biological activity for gut human health f...
Martínez-Gómez, Sergio; Santos-Pereira, Cátia; Pereira, Ana Catarina; Silva, Nuno R. da; Silvério, Sara Isabel Cruz; Alonso, José L.Lemon peels were subjected to hydrothermal treatment and the liquors obtained were processed following several downstream processing schemes obtaining a variety of products with different composition. Up to five products were generated, analysed for their saccharide and polyphenolic content and tested for their antimicrobial, antioxidant and anticancer (cytotoxicity) activities. Unfortunately, none of the produ...
Conventional and alternative microbial sources of food-grade pigments and their...
Silva, Nuno Miguel Sampaio Ribeiro Magalhães; Basto, Bruna; Teixeira, José A.; Silvério, Sara Isabel CruzFood colorant regulation has been updated and improved over the years according to the latest knowledge in the field. Aiming to avoid carcinogenicity and toxicological effects on human health, synthetic pigments have been de-authorized. In recent decades, their gradual substitution with alternative pigments obtained from natural sources has increased. Natural pigments are frequently associated with greener, mor...
Finding novel genes coding for eco-friendly surfactants from hypersaline Iberia...
Santos-Pereira, Cátia; Gomes, Joana S.; Sousa, Joana; Simões, Marta F.; Franco-Duarte, Ricardo; Chaves, Susana R.; Silvério, Sara Isabel CruzSurfactants are tensioactive chemical compounds extensively used worldwide in a myriad of industrial sectors and in our daily lives, being present in numerous products including cosmetics, detergents, fabric softeners, toothpaste, among many others. Millions of tonnes of surfactants are thus manufactured every year. Most commercially available surfactants are non-renewable petroleum-based compounds that can hav...
Pleurotus ostreatus and lentinus sajor-caju laccases for sulfamethoxazole biotr...
Sá, Helena; Michelin, Michele; Silvério, Sara Isabel Cruz; Polizeli, Maria de Lourdes T. M.; Silva, Ana Rita Pereira; Pereira, Luciana; Tavares, T.Sulfamethoxazole (SMX) is an antibiotic commonly used to treat human and animal infections. Therefore, it is one of the most identified pharmaceuticals worldwide in surface water and wastewater treatment plants. This work exploited Pleurotus ostreatus and Lentinus sajor-caju to produce laccase by using agricultural and food residues as substrates, specifically vine pruning and brewer's spent grains. The fungal ...
Epilactose as a promising butyrate-promoter prebiotic via microbiota modulation
Cardoso, Beatriz B.; Amorim, Cláudia Catarina Oliveira; Franco-Duarte, Ricardo; Alves, Joana I.; Barbosa, Sónia G.; Silvério, Sara Isabel CruzEpilactose is a disaccharide composed of galactose and mannose, and it is currently considered an under development prebiotic. In this study, we described the prebiotic potential of epilactose by in vitro fermentation using human fecal inocula from individuals following a Mediterranean diet (DM) or a Vegan diet (DV). The prebiotic effect of epilactose was also compared with lactulose and raffinose, and interest...