The objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BC...
The in vitro embryo production (IVP) efficiency has been limited by oocyte developmental competence and in vitro culture conditions. The use of Brilliant Cresyl Blue (BCB) dye has been described as an alternative method for selection of better quality oocytes. The aim of this work was to evaluate the selection of immature oocytes by BCB dye and the expression of transcripts involved in the transition from genom...
Tese (doutorado)—Universidade de Brasília, Faculdade de Medicina, Programa de Pós- Graduação em Ciências Médicas, 2013.; A adição de soro nos meios de maturação in vitro (MIV) de complexos cumulus-oócito (CCOs) é pratica comum em laboratórios de pesquisa e comercias. A adição de fatores de crescimento ou hormônios nos meios de MIV tem sido uma alternativa ao uso de soro além de proporcionar um maior conheciment...