6 documents found, page 1 of 1
The anti-caspase inhibitor Q-VD-OPH prevents AIDS disease progression in SIV-in...
Laforge, Mireille; Silvestre, Ricardo Jorge Leal; Rodrigues, Vasco; Garibal, Julie; Campillo-Gimenez, Laure; Mouhamad, Shahul; Monceaux, ValérieApoptosis has been proposed as a key mechanism responsible for CD4+ T cell depletion and immune dysfunction during HIV infection. We demonstrated that Q-VD-OPH, a caspase inhibitor, inhibits spontaneous and activation-induced death of T cells from SIV-infected rhesus macaques (RMs). When administered during the acute phase of infection, Q-VD-OPH was associated with (a) reduced levels of T cell death, (b) preser...
Interleukin-27 early impacts Leishmania infantum infection in mice and correlat...
Pérez-Cabezas, Begoña; Cecílio, Pedro; Robalo, Ana Luísa; Silvestre, Ricardo Jorge Leal; Carrillo, Eugenia; Moreno, Javier; Martín, Juan V. SanThe complexity of Leishmania host interactions, one of the main leishmaniasis issues, is yet to be fully understood. We detected elevated IL-27 plasma levels in European patients with active visceral disease caused by Leishmania infantum, which returned to basal levels after successful treatment, suggesting this cytokine as a probable infection mediator. We further addressed this hypothesis recurring to two cla...
Exploiting the antiparasitic activity of naphthalimides derivatives
Silva, Anabela Cordeiro da; Alves, Maria José Chão; Thoo-Lin, Paul Kong; Baptista, Catarina; Noro, Jennifer Martins; Cristo, Gabriela; Gaspar, LuísA set of 1,8-naphtalimides derivatives were synthesized and tested against three protozoans that cause important human diseases: Leishmaniainfantum, Trypanosomabrucei and Trypanosomacruzi. Additionally, toxicity was determined by growth inhibition of THP-1 derived macrophages. The results suggest that chemical modifications in the carbon chain linking the naphthalimide and the substituting groups have different...
Regulation of immunity during visceral Leishmania infection
Rodrigues, Vasco; Silva, Anabela Cordeiro da; Laforge, Mireille; Silvestre, Ricardo Jorge Leal; Estaquier, JêrômeUnicellular eukaryotes of the genus Leishmania are collectively responsible for a heterogeneous group of diseases known as leishmaniasis. The visceral form of leishmaniasis, caused by L. donovani or L. infantum, is a devastating condition, claiming 20,000 to 40,000 lives annually, with particular incidence in some of the poorest regions of the world. Immunity to Leishmania depends on the development of protecti...
Development of a fluorescent based immunosensor for the serodiagnosis of canine...
Sousa, Susana; Cardoso, Luís; Reed, Steven G.; Reis, Alexandre Barbosa; Martins Filho, Olindo Assis; Silvestre, Ricardo; Silva, Anabela Cordeiro daAn accurate diagnosis is essential for the control of infectious diseases. In the search for effective and efficient tests, biosensors have increasingly been exploited for the development of new and highly sensitive diagnostic methods. Here, we describe a new fluorescent based immunosensor comprising magnetic polymer microspheres coated with recombinant antigens to improve the detection of specific antibodies g...
TLR2-Induced IL-10 production impairs neutrophil recruitment to infected tissue...
Andrade, Elva Bonifácio; Alves, Joana; Madureira, Pedro; Oliveira, Liliana; Ribeiro, Adília; Silva, Anabela Cordeiro da; Neves, Margarida CorreiaSepsis is the third most common cause of neonatal death, with Group B Streptococcus (GBS) being the leading bacterial agent. The pathogenesis of neonatal septicemia is still unsolved. We described previously that host susceptibility to GBS infection is due to early IL-10 production. In this study, we investigated whether triggering TLR2 to produce IL-10 is a risk factor for neonatal bacterial sepsis. We observe...