7 documents found, page 1 of 1
Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia qu...
Silva, Martinho de Almeida E.; Costa, Guilherme M.J.; Lacerda, Samyra Maria Santos N.Nassif; Brandão-Dias, Pedro Ferreira PintoFish germ cell transplantation presents several important potential applications for aquaculture, including the preservation of germplasm from endangered fish species with high genetic and commercial values. Using this technique in studies developed in our laboratory with adult male Nile tilapias (Oreochromis niloticus), all the necessary procedures were successfully established, allowing the production of func...
Neuropharmacological effects of Phoneutria nigriventer venom on astrocytes
Raposo, Catarina [UNESP]; Bjorklund, Ulrika; Kalapothakis, Evanguedes; Biber, Bjorn; Cruz-Hofling, Maria Alice da; Hansson, ElisabethMade available in DSpace on 2018-11-26T16:33:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-06-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Edit Jacobson's Foundation in Gothenburg, Sweden; Sahlgrenska University Hospital in Gothenburg, Sweden; AFA Insurance, Stockholm, Sweden; Bites from genus Phoneutria (Cte...
Characterization of a new Autographa californica multiple nucleopolyhedrovirus ...
Ribeiro, Bergmann Morais; Generino, Ana Paula Montenegro; Acácio, Cláudia Natércia Lima; Kalapothakis, Evanguedes; Báo, Sônia NairIn the very late phase of baculovirus infection, virions are occluded in a crystalline matrix called polyhedra, which is mainly composed of polyhedrin. This protein is highly conserved among baculoviruses and changes in its amino acid sequence may lead to mutant polyhedra. During the purification of an AcMNPV recombinant virus, a mutant virus was isolated. Structural and ultrastrutural analysis by light and tra...
Purification, amino-acid sequence and partial characterization of two toxins wi...
Pimenta, Adriano Monteiro de Castro; Eauclaire, Marie France Martin; Rochat, Hervé; Figueiredo, Suely Gomes de; Kalapothakis, EvanguedesWe report here the isolation by a two-step chromatographic procedure of two new toxins from the South American scorpion Tityus bahiensis. Their amino-acid sequences and some of their biological features were established. The two toxins have different biological properties. Toxin TbIT-I had almost no activity or pharmacological effects in vertebrate tissues whereas it was lethal to house ¯ies (LD50 80.0 ng/house...
Screening of expression libraries using ELISA : identification of immunogenic p...
Kalapothakis, Evanguedes; Jardim, S.; Magalhães, Ana Cristina Ribeiro; Mendes, Thais Melo; Marco, Luiz de; Afonso, Luís Carlos CroccoThe present report describes the use of ELISA with cDNA expression libraries in the identification of immunogenic proteins. The methodology described was applied using libraries constructed with mRNA isolated from Tityus serrulatus and Tityus bahiensis venom glands. In addition we describe for the first time the sequence of a neurotoxin from Tityus bahiensis venom gland named TbTx5 whose amino acid sequencing s...
A New and Fast Technique to Generate Offspring after Germ Cells Transplantation...
Lacerda, Samyra M. S. N.; Batlouni, Sergio R. [UNESP]; Costa, Guilherme M. J.; Segatelli, Tania M.; Quirino, Bruno R.; Queiroz, Bruno M.Made available in DSpace on 2013-08-28T14:08:22Z (GMT). No. of bitstreams: 1 WOS000278017300013.pdf: 7512680 bytes, checksum: 985d975296b48c7197a127fcd01db2ed (MD5); Made available in DSpace on 2013-09-30T19:22:31Z (GMT). No. of bitstreams: 2 WOS000278017300013.pdf: 7512680 bytes, checksum: 985d975296b48c7197a127fcd01db2ed (MD5) WOS000278017300013.pdf.txt: 44589 bytes, checksum: 9f10ff7988f2d6dbe5fddf41647135fe...
Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effect...
Dutra, Alexandre Augusto de Assis; Sousa, Lucas Oliveira; Resende, Rodrigo Ribeiro; Brandão, Rogélio Lopes; Kalapothakis, EvanguedesHere, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LT...