16 documents found, page 1 of 2
Microfluidic flow-focusing for size-controlled formation of cubosomes
Ferreira, Celso Manuel Alves; Barros, Margarida Lemos; Fornasier, Marco; Chícharo, Alexandre; Botelho, Cláudia M.; Real Oliveira, Maria Elisabete C. D.Cubosomescolloidal dispersions of lipid inverted bicontinuous cubic phasesare promising nanosystems for advanced drug delivery applications. Here, we report on a microfluidic hydrodynamic flow-focusing approach for solvent exchange that enables the preparation of cubosomes with tunable size. The approach involves a preliminary step where the lipid phytantriol, the main building block, is first dissolved in etha...
A versatile nanocarrierCubosomes, characterization, and applications
Oliveira, Cristiana; Ferreira, Celso; Vieira, P.; Paris, Juan L.; Gaspar, Ricardo; Silva, Bruno F. B.; Teixeira, J. A.; Santos, Pedro Miguel FerreiraThe impact of nanotechnology on the exponential growth of several research areas, particularly nanomedicine, is undeniable. The ability to deliver active molecules to the desired site could significantly improve the efficiency of medical treatments. One of the nanocarriers developed which has drawn researchers’ attention are cubosomes, which are nanosized dispersions of lipid bicontinuous cubic phases in water,...
In Vitro CRISPR/Cas9 Transfection and Gene-Editing Mediated by Multivalent Cati...
Sousa, Diana Andrade; Gaspar, Ricardo; Ferreira, Celso J. O.; Baltazar, Fátima; Rodrigues, L. R.; Silva, Bruno F. B.Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated nuclease 9 (Cas9) gene-editing offers exciting new therapeutic possibilities for disease treatment with a genetic etiology such as cancer, cardiovascular, neuronal, and immune disorders. However, its clinical translation is being hampered by the lack of safe, versatile, and effective nonviral delivery systems. Herein we rep...
Liposomes loaded with the pneumococcal endolysin MSlys: From in vitro character...
Silva, Maria Daniela; Paris, Juan Luis; Ray, Kaelin; Gama, F. M.; Silva, Bruno F. B.; Remenshneider, A. K.; Sillankorva, SannaIntroduction: The increasing antibiotic resistance triggered interest in novel antimicrobials as well as delivery systems that allow a topical and targeted delivery. However, biological barriers, such as the skin or the tympanic membrane (TM), may hinder the success of the therapy. Drug permeation has been extensively studied in the context of transdermal delivery, but only recently started to be explored for t...
Exploring endolysin-loaded liposomes for a transtympanic treatment of S. pneumo...
Silva, Maria Daniela; Paris, Juan Luis; Ray, Kaelin; Gama, F. M.; Silva, Bruno F. B.; Remenshneider, A. K.; Sillankorva, SannaOtitis media, the main reason for which antibiotics are prescribed in childhood, is often caused by Streptococcus pneumoniae. The exogenous use of recombinantly produced endolysins, peptidoglycan hydrolases encoded by bacteriophages at the end of their lytic cycle, have been shown to be very effective against this pathogen. To increase bioavailability, and consequently reduce the probability of a recurrent or c...
Cationic lipid-based formulations for encapsulation and delivery of anti-EFG1 2...
Araújo, Daniela Eira; Gaspar, Ricardo; Mil-Homens, Dalila; Henriques, Mariana; Silva, Bruno F. B.; Silva, Sónia CarinaThe effective protection and delivery of antisense oligomers to its site of action is a challenge without an optimal strategy. Some of the most promising approaches encompass the complexation of nucleic acids, which are anionic, with liposomes of fixed or ionizable cationic charge. Thus, the main purpose of this work was to study the complexation of cationic liposomes with anti-EFG1 2′OMe oligomers and evaluate...
pH-sensitive nanoliposomes for passive and CXCR-4-mediated marine yessotoxin de...
Vieira, Ana M. G.; Silvestre, Oscar F.; Silva, Bruno F. B.; Ferreira, Celso J. O.; Lopes, Ivo Edgar Araújo; Gomes, Andreia C; Espiña, BegoñaBackground: Yessotoxin (YTX), a marine-derived drug, was encapsulated in PEGylated pH-sensitive nanoliposomes, covalently functionalized (strategy I) with SDF-1α and by nonspecific adsorption (strategy II), to actively target chemokine receptor CXCR-4. Methods: Cytotoxicity to normal human epithelial cells (HK-2) and prostate (PC-3) and breast (MCF-7) adenocarcinoma models, with different expression levels of C...
Cellular delivery of CRISPR/Cas9 plasmid using multivalent cationic liposomes
Sousa, Diana Andrade; Gaspar, Ricardo; Ferreira, Celso J. O.; Baltazar, Fátima; Silva, Bruno F. B.; Rodrigues, L. R.[Excerpt] Background: Gene therapy techniques have been aimed at mitigating disease features of recessive and dominant disorders, as well as several cancers, cardiovascular, neuronal and immune diseases [1]. In recent years, the clustered regularly interspaced short palindromic repeats (CRISPR) and Cas9 protein system has been gaining recognition as a revolutionary tool for gene therapy. However, the implementa...
Sustained release of a Streptococcus pneumoniae endolysin from liposomes for po...
Silva, Maria Daniela Ferreira; Paris, Juan L.; Gama, F. M.; Silva, Bruno F. B.; Sillankorva, SannaLocal delivery of antimicrobials for otitis media treatment would maximize therapeutic efficacy while minimizing side effects. However, drug transport across the tympanic membrane in the absence of a delivery system is challenging. In this study, the MSlys endolysin was encapsulated in deformable liposomes for a targeted treatment of S. pneumoniae, one of the most important causative agents of otitis media. MSl...
Influence of the media ionic strength on the formation and in vitro biological ...
Coelho, Filipe; Botelho, C. M.; Paris, Juan L.; Marques, Eduardo F.; Silva, Bruno F. B.Cationic polymer-DNA complexes, or polyplexes, have been subject to intensive investigation as potentially efficient non-viral systems for gene therapy. Yet the effects of ionic strength, a physiologically relevant parameter, on the formation, physicochemical properties (e.g. size and colloidal stability) and transfection efficiency of polyplexes are still poorly investigated and understood. In this work, we an...