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The effect of the Messinian salinity crisis on the early diversification of the...

Costa, Gonçalo J.; Nunes, Vera L.; Marabuto, Eduardo; Mendes, Raquel; Silva, Diogo N.; Pons, Pere; Bas, Josep M.; Hertach, Thomas; Paulo, Octávio S.

The current distribution patterns of many Mediterranean species are often a consequence of large and impactful past geoclimatic events, such as the Messinian Salinity Crisis (MSC) and the Quaternary glacial cycles. Cicadas are flying insects with poor dispersal ability, which have experienced intense local differentiation in the Mediterranean, where the genus Tettigettalna has surfaced as a biogeographic model....


DEN-IM: dengue virus genotyping from amplicon and shotgun metagenomic sequencing

Mendes, Catarina Inês; Lizarazo, Erley; Machado, Miguel P.; Silva, Diogo N.; Tami, Adriana; Ramirez, Mário; Couto, Natacha; Rossen, John W. A.

Dengue virus (DENV) represents a public health threat and economic burden in affected countries. The availability of genomic data is key to understanding viral evolution and dynamics, supporting improved control strategies. Currently, the use of high-throughput sequencing (HTS) technologies, which can be applied both directly to patient samples (shotgun metagenomics) and to PCR-amplified viral sequences (amplic...


Structure_threader: an improved method for automation and parallelization of pr...

Pina-Martins, Francisco; Silva, Diogo N.; Fino, Joana; Paulo, Octávio S.

Structure_threader is a program to parallelize multiple runs of genetic clustering software that does not make use of multithreading technology (structure, fastStructure and MavericK) on multicore computers. Our approach was benchmarked across multiple systems and displayed great speed improvements relative to the single-threaded implementation, scaling very close to linearly with the number of physical cores u...


Legitimacy and implications of reducing Colletotrichum kahawae to subspecies in...

Batista, Dora; Silva, Diogo N.; Cabral, Ana; Pires, Ana S.; Loureiro, Andreia; Guerra-Guimarães, Leonor; Pereira, Ana P.; Azinheira, Helena


The coffee leaf rust pathogen Hemileia vastatrix: one and a half centuries arou...

Talhinhas, Pedro; Batista, Dora; Diniz, Inês; Vieira, Ana; Silva, Diogo N.; Loureiro, Andreia; Tavares, Sílvia; Pereira, Ana Paula; Azinheira, Helena G.

Taxonomy and History: Hemileia vastatrix Berk. and Broome (Basidiomycota, Pucciniales) was described in 1869 in eastern Africa and Ceylon as the agent of coffee leaf rust and has spread to all coffee cultivation areas worldwide. Major disease outbreaks in Asia, Africa and America caused and continue to cause severe yield losses, making this the most important disease of Arabica coffee, a cash crop for many trop...


Structure_threader: An improved method for automation and parallelization of pr...

Pina-Martins, Francisco; Silva, Diogo N.; Fino, Joana; Paulo, Octávio S.

Structure_threader is a program to parallelize multiple runs of genetic clustering software that does not make use of multithreading technology (structure, fastStructure and MavericK) on multicore computers. Our approach was benchmarked across multiple systems and displayed great speed improvements relative to the single-threaded implementation, scaling very close to linearly with the number of physical cores u...


New mitochondrial and nuclear evidences support recent demographic expansion an...

Rodrigues, Ana S. B.; Silva, Sara E.; Marabuto, Eduardo; Silva, Diogo N.; Wilson, Mike R.; Thompson, Vinton; Yurtsever, Selçuk; Halkka, Antti

Philaenus spumarius is a widespread insect species in the Holarctic region. Here, by focusing on the mtDNA gene COI but also using the COII and Cyt b genes and the nuclear gene EF-1α, we tried to explain how and when its current biogeographic pattern evolved by providing time estimates of the main demographic and evolutionary events and investigating its colonization patterns in and out of Eurasia. Evidence of ...


New Mitochondrial and Nuclear Evidences Support Recent Demographic Expansion an...

Rodrigues, Ana S. B.; Silva, Sara E.; Marabuto, Eduardo; Silva, Diogo N.; Wilson, Mike R.; Thompson, Vinton; Yurtsever, Selçuk; Halkka, Antti

Philaenus spumarius is a widespread insect species in the Holarctic region. Here, by focusing on the mtDNA gene COI but also using the COII and Cyt b genes and the nuclear gene EF-1α, we tried to explain how and when its current biogeographic pattern evolved by providing time estimates of the main demographic and evolutionary events and investigating its colonization patterns in and out of Eurasia. Evidence of ...


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