12 documents found, page 1 of 2
Analysis of terpenes in cannabis by GC-MS: method development and its applicati...
Capita, Albertina M.; Morais, Ana F.; Dinis-Oliveira, Ricardo Jorge; Ribeiro, Carlos J. A.; Silva, Eduarda M. P.Background: In addition to cannabinoids, key bioactive compounds in cannabis, terpenes and terpenoids, also have therapeutic importance, potentially working synergistically through their entourage effect for the medicinal efficacy of this plant [1,2]. Besides their therapeutic advantages, they are also responsible for the characteristic aroma of numerous varieties of cannabis, ranging from citrus to woody...
GC-MS-Based Study of 14 Cannabinoids Separation in Cannabis sativa L. Extracts ...
Nunes, Bruna A. S.; Capita, Albertina M.; Morais, Ana F.; Dinis-Oliveira, Ricardo J.; Ribeiro, Carlos J. A.; Silva, Eduarda M. P.Background: Cannabinoids from Cannabis sativa L. are increasingly studied due to their potential as medicinal drugs, offering therapeutic benefits such as pain relief and anti-inflammatory effects, while also raising concerns as substances of abuse due to their psychoactive properties [1,2]. Gas chromatography-mass spectrometry (GC-MS) is a powerful tool for identifying and quantifying cannabinoids, offering hi...
Exploiting kinetic features of ORAC assay for evaluation of radical scavenging ...
Carvalho, Joana R. B.; Meireles, Andreia N.; Marques, Sara S.; Gregório, Bruno J. R.; Ramos, Inês I.; Silva, Eduarda M. P.; Barreiros, LuisaThe analysis and interpretation of data retrieved from Oxygen Radical Absorbance Capacity (ORAC) assays represent a challenging task. ORAC indexes originate from different mathematical approaches often lacking correct elucidation of kinetic features concerning radical scavenging reactions by antioxidant compounds. In this work, the expression of ORAC values as area under fluorescein (FL) decay curves (AUC) and ...
Acetonitrile adducts of tranexamic acid as sensitive ions for quantification at...
Silva, Eduarda M. P.; Barreiros, Luísa; Fernandes, Sara R.; Sá, Paula; Ramalho, João P. Prates; Segundo, Marcela A.The quantitative analysis of pharmaceuticals in biomatrices by liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is often hampered by adduct formation. The use of the molecular ion resulting from solvent adducts for quantification is uncommon, even if formed in high abundance. In this work, we propose the use of a protonated acetonitrile adduct for the quantitati...
Analytical strategies based on tandem mass spectrometry detection for quantific...
Barreiros, Luisa; Fernandes, Sara R.; Machado, Sandia; Silva, Eduarda M. P.; Segundo, Marcela A.Fast and accurate analysis, providing reliable results at trace concentration levels, is a current demand of the modern world. This pressure is justifiable in limit situations but also in our daily life, for instance when waiting for a diagnosis based on lab results in a hospital or when wondering about the quality of water running from our taps. During the last years, tandem mass spectrometry (MS/MS) based tec...
Chromatographic method for the simultaneous quantification of dapsone and clofa...
Machado, Sandia; Fernandes, Sara; Chaves, Luise L.; Lima, Sofia A. C.; Silva, Eduarda M. P.; Barreiros, Luisa; Reis, Salette; Segundo, Marcela A.The low bioavailability and nonspecific distribution of dapsone and clofazimine, commonly applied in combination for the treatment of leprosy, can produce toxic effects. Nanotechnological approaches enhance the delivery of these drugs. Therefore, a high-performance liquid chromatography method was developed for the simultaneous determination of dapsone and clofazimine loaded in nanoformulations for quality cont...
Evaluation of enzymatic digestion conditions for determination of immunoglobuli...
Guerra, Gabriela S.; Ramos, Inês I.; Barreiros, Luísa; Silva, Eduarda M. P.; Segundo, Marcela A.Immunoassays, namely ELISA, have been the standard method for detecting clinically significant immunoglobulins (Igs). They are based on Ig-antigen interaction, often suffering interference from matrix components. New analytical approaches using detection by tandem mass spectrometry (MS/MS) search for fundamental structure information of target Igs based on protein features. In fact, there are few examples of qu...
Quantification of tranexamic acid in human plasma: development and validation o...
Barreiros, Luisa; Amoreira, Júlia L.; Machado, Sandia; Fernandes, Sara R.; Silva, Eduarda M. P.; Sá, Paula; Kietaibl, Sibylle; Segundo, Marcela A.Tranexamic acid (TXA), an antifibrinolytic drug with the ability to inhibit lysine binding at plasminogen receptors, can be used in different settings such as trauma, cardiac surgery, major orthopedic surgery, obstetric when perioperative bleeding is concerned [1]. Effective methods for determination of TXA in biological samples are still required to understand the pharmacokinetics and pharmacodynamics of this ...
Simultaneous determination of dapsone and clofazimine in nanoformulations by HPLC
Fernandes, Sara R.; Fernandes, Sara; Chaves, Luíse L.; Lima, Sofia A. C.; Silva, Eduarda M. P.; Barreiros, Luísa; Reis, Salette; Segundo, Marcela A.The multidrug therapy with dapsone (DAP) and clofazimine (CLZ) is known as an effective treatment against Mycobacterium leprae. However, the low bioavailability and non-specific distribution can reduce therapy efficacy and produce side effects. The use of nanotechnological approaches was explored as a promising carrier for delivery enhancement of these drugs. Therefore, a simple and precise highperformance liqu...
HPLC-MS/MS method for quantification of the neuropeptide Y Y1 receptor antagoni...
Barreiros, Luisa; Silva, Eduarda M. P.; Alencastre, Inês S.; Lamghari, Meriem; Segundo, Marcela A.; Barreiros, LuisaNeuropeptide Y (NPY) is involved in various physiological processes, including the regulation of feeding behavior and energy homeostasis. NPY activates different receptors in several brain regions. Recently, Y1 receptor (Y1R) has arisen as a potential regulator in the local control of bone turnover suggesting that an antireceptor strategy may be a useful therapeutic approach to prevent and/or reverse bone loss....