4 documents found, page 1 of 1
Cytotoxicity and Mitochondrial Effects of Phenolic and Quinone-Based Mitochondr...
Fernandes, Carlos; Videira, Afonso J. C.; Veloso, Caroline D.; Benfeito, Sofia; Soares, Pedro; Martins, João D.; Gonçalves, Beatriz NunesMitochondriotropic antioxidants (MC3, MC6.2, MC4 and MC7.2) based on dietary antioxidants and analogs (caffeic, hydrocaffeic, trihydroxyphenylpropanoic and trihydroxycinnamic acids) were developed. In this study, we evaluate and compare the cytotoxicity profile of novel mitochondria-targeted molecules (generally known as MitoCINs) on human HepG2 and differentiated SH-SY5Y cells with the quinone-based mitochondr...
Oxidative Stress in Amyotrophic Lateral Sclerosis: Pathophysiology and Opportun...
Cunha-Oliveira, Teresa; Montezinho, Liliana; Mendes, Catarina; Firuzi, Omidreza; Saso, Luciano; Oliveira, Paulo J.; Silva, Filomena S. G.Amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig's disease or Charcot disease, is a fatal neurodegenerative disease that affects motor neurons (MNs) and leads to death within 2-5 years of diagnosis, without any effective therapy available. Although the pathological mechanisms leading to ALS are still unknown, a wealth of evidence indicates that an excessive reactive oxygen species (ROS) production ...
Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sul...
Silva, Filomena S. G.; Starostina, Irina G.; Ivanova, Vilena V.; Rizvanov, Albert A.; Oliveira, Paulo J.; Pereira, Susana P.The identification of rapid, reliable, and highly reproducible biological assays that can be standardized and routinely used in preclinical tests constitutes a promising approach to reducing drug discovery costs and time. This unit details a tandem, rapid, and reliable cell viability method for preliminary screening of chemical compounds. This assay measures metabolic activity and cell mass in the same cell sam...
The antiestrogen 4-hydroxytamoxifen protects against isotretinoin-induced perme...
Silva, Filomena S. G.; Ribeiro, Mariana P. C.; Santos, M. S.; Rocha-Pereira, Petronila; Santos-Silva, Alice; Custódio, José B. A.The combination of isotretinoin (13-cis-retinoic acid) with antiestrogens seems to be a promising strategy for cancer chemotherapy. The aim of the study was to evaluate the effects of isotretinoin alone or in combination with 4-hydroxytamoxifen (OHTAM) and with its prodrug tamoxifen (TAM), on the functions of rat liver mitochondria, i.e., mitochondrial permeability transition (MPT), bioenergetic functions and a...