42 documents found, page 1 of 5
Enhancing biomarker detection in human serum for lung cancer diagnosis: aqueous...
Rosa, Marguerita E.; Mendes, Maria S. M.; Belchior, Diana C. V.; Coutinho, João A. P.; Silva, Francisca A. e; Freire, Mara G.Analysing biomarkers in human serum could be used as an effective and less invasive approach for the diagnosis of lung cancer; however, biomarker detection reliability is highly limited due to matrix effects. Herein, aqueous biphasic systems (ABS) are studied as platforms for human serum pretreatment, allowing the simultaneous depletion of high abundant proteins and biomarker extraction. By using ABS varying th...
The use of thermoreversible ionic liquid-based aqueous biphasic systems as an i...
Silva, Luís C. V.; Sousa, Maria I.; Correia, Andreia; Freire, Mara G.; Silva, Francisca A. e; Pedro, Augusto Q.COVID-19 reinforced the potential of messenger RNA (mRNA) vaccines as effective platforms to control disease outbreaks, given their rapid development, safety and efficacy. Nevertheless, mRNA manufacturing can be optimized by combining production with initial clarification steps. This work aims to integrate mRNA production and initial clarification steps, using thermoreversible ionic liquid-based aqueous biphasi...
Tackling water contamination by oncologic drugs: supported ionic liquids as sus...
Francisco, Rafael; Monteiro, Bruna; Santos, Maria J.; Silva, Francisca A. e; Venâncio, Cátia; Neves, Márcia C.; Lopes, Isabel; Sousa, Ana C. A.Due to the increasing incidence of cancer, the consumption of highly toxic oncological drugs is continuously growing. Given the current lack of efficient technologies to remove/treat these toxic drugs in wastewater treatment plants, the environmental quality is compromised, and aquatic organisms are at risk. To address this critical environmental burden, a new strategy based on supported ionic liquids (SILs) fo...
Sistemas aquosos bifásicos e dispositivos microfluídicos para o pré-tratamento ...
Silva, Francisca A. eA análise de biomarcadores em fluidos humanos representa uma estratégia promissora para o diagnóstico precoce, rastreio e prognóstico de vários tipos de cancro. Contudo, a complexidade dos fluidos humanos e a baixa concentração dos biomarcadores tumorais limita a qualidade, simplicidade e custo da análise, conduzindo a resultados falsos positivos/negativos e dificultando a aplicação a uma escala global. De form...
Development of an integrated messenger RNA manufacturing process using thermore...
Sousa, Maria I.; Silva, Luís; Freire, Mara G.; Tavares, Ana P. M.; Silva, Francisca A. e; Pedro, Augusto Q.The promising potential of messenger RNA (mRNA) vaccines as effective approaches to contain the dissemination of infectious diseases was fully disclosed during the combat to the COVID-19 pandemic. Over conventional vaccines, mRNA-based vaccines exhibit improved safety and efficacy profiles, and the possibility of repeatedly administration [1]. However, the manufacturing of mRNA vaccines is complex, costly and r...
Using thermoreversible aqueous biphasic systems and ionic liquids to improve th...
Sousa, Maria I.; Silva, Luís C. V.; Correia, Andreia; Freire, Mara G.; Silva, Francisca A. e; Pedro, Augusto Q.If there is one thing the COVID 19 pandemic has taught us is the enormous potential of messenger RNA (mRNA) vaccines as an effective tool to contain infectious disease outbreaks. Over conventional vaccines, there are several advantages to mRNA vaccines, namely improved safety and efficacy, and the possibility of repeatedly administration [1]. However, mRNA nanomedicine production is still a complex and expensiv...
Improving the manufacturing of mRNA nanomedicines using thermoreversible aqueou...
Sousa, Maria I.; Silva, Luís C. V.; Correia, Andreia; Freire, Mara G.; Silva, Francisca A. e; Pedro, Augusto Q.It is undeniable the potential of messenger RNA (mRNA) vaccines as effective tools to manage infectious disease outbreaks. However, mRNA nanomedicines production is complex and expensive, requiring improved technologies to produce stable and widely accessible products, whilst meeting required production times and fulfilling market demands. Ionic liquids (ILs) are composed of organic cations and organic or inorg...
Combined Use of Ionic Liquid-Based Aqueous Biphasic Systems and Microfluidic De...
Flora, Filipa C.; Relvas, Sofia B.; Silva, Francisca A. e; Freire, Mara G.; Chu, Virginia; Conde, João PedroProstate cancer (PCa) is one of the cancer types that most affects males worldwide and is among the highest contributors to cancer mortality rates. Therefore, there is an urgent need to find strategies to improve the diagnosis of PCa. Microtechnologies have been gaining ground in biomedical devices, with microfluidics and lab-on-chip systems potentially revolutionizing medical diagnostics. In this paper, it is ...
Using three-phase partitioning for the purification and recovery of antibodies ...
Capela, Emanuel V.; Magnis, Ilaria; Rufino, Ana F.C.S.; Torres-Acosta, Mário A.; Aires-Barros, M. Raquel; Coutinho, João A.P.; Azevedo, Ana M.Antibodies, in particular immunoglobulin G (IgG), are one of the biopharmaceutical industry highflyers, with relevance for the treatment of several diseases. However, the recovery of antibodies from complex biological media with high quality and purity is difficult and requires multi-step and expensive approaches. Herein, we propose a cost-effective approach using three-phase partitioning (TPP) systems based on...
Extraction of breast cancer biomarkers from human serum using ionic liquid-base...
Mendes, Maria S. M.; Souza, Maria C.; Freire, Mara G.; Silva, Francisca A. eNo abstract available.