24 documents found, page 1 of 3

Antibiotic Resistance and Public Knowledge

The discovery of antibiotics was one of the most landmark discoveries of the twentieth century, but their inappropriate use fostered the development of resistant bacteria, thus becoming a threat to public health. In Portugal, the population’s literacy on this subject is not known, and as such, a study was carried out to assess the knowledge of the population in the central interior region and to assess adherenc...

Relatório de Estágio Curricular – Gigarte _ Agência de Comunicação (Covilhã)

O presente relatório diz respeito ao estágio curricular que realizei na Gigarte – Agência de Comunicação, situada na Covilhã, com vista à obtenção do diploma de Licenciada em Comunicação e Relações Públicas, pelo Instituto Politécnico da Guarda. Durante o período de estágio, tive a oportunidade de desenvolver diversas funções incluindo a gestão de redes sociais para três clientes da agência, pesquisa e criação ...

Jorge, L. (2019). O livro das tréguas. Dom Quixote. Lisboa. 104 pp.

Narratives and senses of the De Volta para Casa Program (Back Home Program): we...

Este artigo apresenta e resume os principais achados em relação às repercussões do Programa de Volta para Casa nos territórios existenciais de seus beneficiários. Para isso, insere-se no bojo dos estudos internacionais e nacionais sobre o impacto das políticas públicas e avaliação do próprio beneficiário sobre elas. Dado o volume e a extensão dos dados, optou-se por apresentar análises a partir das narrativas c...

Percepções e perspectivas discentes nos cursos de pós-graduação em Escrita Cria...

Este artigo se propõe a analisar os dados parciais obtidos pelo grupo de pesquisa Escrita Criativa na Academia: a formação do escritor, que desenvolve, desde 2015, uma pesquisa cujo objetivo geral é analisar a estrutura curricular e as condições de ensino oferecidas pelos cursos de mestrado e doutorado voltados para a formação do escritor, do pesquisador e do docente na área de Escrita Criativa da PUCRS. Preten...

Efficiency of RAFT-synthesized PDMAEMA in gene transfer to the retina

Gene therapy has long been heralded as the new hope to evolve from symptomatic care of genetic pathologies to a full cure. Recent successes in using gene therapy for treating several ocular and haematopoietic pathologies have shown the great potential of this approach that, in the early days, relied on the use of viral vectors, which were considered by many to be undesirable for human treatment. Therefore, ther...

Aliskiren inhibits the renin-angiotensin system in retinal pigment epithelium c...

Observations of increased angiotensin II levels and activation of the (pro)renin receptor in retinopathies support the role of ocular renin-angiotensin system (RAS) in the development of retinal diseases. While targeting RAS presents significant therapeutic potential, current RAS-based therapies are ineffective halting the progression of these diseases. A new class of drugs, the direct renin inhibitors such as ...

Combining hyaluronic acid with chitosan enhances gene delivery

The low gene transfer efficiency of chitosan-DNA polyplexes is a consequence of their high stability and consequent slow DNA release. The incorporation of an anionic polymer is believed to loosen chitosan interactions with DNA and thus promote higher transfection efficiencies. In this work, several formulations of chitosan-DNA polyplexes incorporating hyaluronic acid were prepared and characterized for their ge...

Sustained gene expression in the retina by improved episomal vectors

Gene and cellular therapies are nowadays part of therapeutic strategies for the treatment of diverse pathologies. The drawbacks associated with gene therapy-low levels of transgene expression, vector loss during mitosis, and gene silencing-need to be addressed. The pEPI-1 and pEPito family of vectors was developed to overcome these limitations. It contains a scaffold/matrix attachment region, which anchors its ...

Cationic polyene phospholipids as DNA carriers for ocular gene therapy

Recent success in the treatment of congenital blindness demonstrates the potential of ocular gene therapy as a therapeutic approach. The eye is a good target due to its small size, minimal diffusion of therapeutic agent to the systemic circulation, and low immune and inflammatory responses. Currently, most approaches are based on viral vectors, but efforts continue towards the synthesis and evaluation of new no...