13 documents found, page 1 of 2
Enhancement of the solubility of 5-fluorouracil through encapsulation within β-...
Machado, Mónica; Silva, Gabriela A.; Ferreira, Joana; Pinto, Luís Abegão; Ferreira, QuirinaThis journal is © The Royal Society of Chemistry.; 5-Fluorouracil (5-FU) is an antimetabolite agent used in chemotherapy and wound healing management, with low solubility and bioavailability. To overcome these challenges, this study explores the encapsulation of 5-FU within β-cyclodextrin (β-CD). The inclusion complex was prepared using the solubilization process, and its stability was evaluated under a variety...
Correlation between hyperglycemia and glycated albumin with retinopathy of prem...
Almeida, Ana C.; Silva, Gabriela A.; Santini, Gabriele; Brízido, Margarida; Correia, Miguel; Coelho, Constança; Borrego, Luís MiguelTo determine the association between hyperglycemia, glycated albumin (GlyA) and retinopathy of prematurity (ROP). Prospective study of all infants under ROP screening from March 2017 to July 2019. All demographic, clinical and laboratory data were collected. Glucose was measured at birth and every 8 h for the first week and serum GlyA was evaluated at birth, 1st, 2nd and 4th weeks after birth. Reference range f...
Serum levels of placental growth factor reflect the severity of retinopathy of ...
Almeida, Ana C.; Bitoque, Diogo B.; Martins, Catarina; Coelho, Constança; Borrego, Luís Miguel; Silva, Gabriela A.Retinopathy of prematurity (ROP) is a neovascular disorder of the immature retina and a leading cause of preventable blindness worldwide.1 A two-phase hypothesis for ROP pathogenesis has been suggested.1 Phase one is vaso-obliteration, where physiological vascularity is compromised and retinal vascular development is delayed by a hyperoxic environment. This induces vasoconstriction, with decreased levels of vas...
Correlation between hyperglycemia and glycated albumin with retinopathy of prem...
Almeida, Ana C.; Silva, Gabriela A.; Santini, Gabriele; Brízido, Margarida; Correia, Miguel; Coelho, Constança; Borrego, Luís MiguelTo determine the association between hyperglycemia, glycated albumin (GlyA) and retinopathy of prematurity (ROP). Prospective study of all infants under ROP screening from March 2017 to July 2019. All demographic, clinical and laboratory data were collected. Glucose was measured at birth and every 8 h for the first week and serum GlyA was evaluated at birth, 1st, 2nd and 4th weeks after birth. Reference range f...
Human-derived NLS enhance the gene transfer efficiency of chitosan
Bitoque, Diogo; Morais, Joana; Oliveira, Ana; Sequeira, Raquel L.; Calado, Sofia; Fortunato, Tiago M.; Simão, Sónia; Rosa Da Costa, AnaNuclear import is considered as one of the major limitations for non-viral gene delivery systems and the incorporation of nuclear localization signals (NLS) that mediate nuclear intake can be used as a strategy to enhance internalization of exogenous DNA. In this work, human-derived endogenous NLS peptides based on insulin growth factor binding proteins (IGFBP), namely IGFBP-3 and IGFBP-5, were tested for their...
Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 Akita
Carvalho, Filipe R.; Calado, Sofia M.; Silva, Gabriela A.; Diogo, Gabriela S.; Moreira-Silva, Joana; Reis, R. L.; Cancela, M. Leonor; Gavaia, Paulo J.Type 1 diabetes mellitus (T1DM) has been associated to several cartilage and bone alterations including growth retardation, increased fracture risk, and bone loss. To determine the effect of long term diabetes on bone we used adult and aging Ins2(Akita) mice that developed T1DM around 3-4 weeks after birth. Both Ins2(Akita) and wild-type (WT) mice were analyzed at 4, 6, and 12 months to assess bone parameters s...
Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)
Pires De Carvalho, Filipe Ricardo; Calado, Sofia; Silva, Gabriela A.; Diogo, Gabriela S.; Moreira da Silva, Joana; Reis, Rui L.; Cancela, M. LeonorType 1 diabetes mellitus (T1DM) has been associated to several cartilage and bone alterations including growth retardation, increased fracture risk, and bone loss. To determine the effect of long term diabetes on bone we used adult and aging Ins2 Akita mice that developed T1DM around 3-4 weeks after birth. Both Ins2 Akita and wild-type (WT) mice were analyzed at 4, 6, and 12 months to assess bone parameters suc...
Aliskiren decreases oxidative stress and angiogenic markers in retinal pigment ...
S, Simão; Santos, Daniela F.; Silva, Gabriela A.There is growing evidence on the role of ocular renin-angiotensin system (RAS) in the development of diabetic retinopathy (DR), particularly due to the trigger of oxidative stress and angiogenesis. Despite this there is no effective RAS-based therapy in DR capable of preventing retinal damage induced by RAS activation. We recently described that retinal pigment epithelium (RPE) cells express the main components...
Non-viral strategies for ocular gene delivery
V. Oliveira, Ana; Rosa Da Costa, Ana; Silva, Gabriela A.The success of gene therapy relies on efficient gene transfer and stable transgene expression. The in vivo efficiency is determined by the delivery vector, route of administration, therapeutic gene, and target cells. While some requirements are common to several strategies, others depend on the target disease and transgene product. Consequently, it is unlikely that a single system is suitable for all applicatio...
Evaluation of cystamine-modified hyaluronic acid/chitosan polyplex as retinal g...
Oliveira, Ana V.; Marcelo, Adriana; Costa, Ana M. Rosa da; Silva, Gabriela A.A successful gene therapy approach can prevent or treat congenital and acquired diseases. However, there is still no ideal non-viral vector for gene delivery in a safe and timely manner. In this report the anionic polymer hyaluronic acid (HA) was investigated as a potential vector for gene therapy. Due to its intrinsic characteristics it constitutes an excellent candidate to deliver therapeutic genes, pending t...