3 documents found, page 1 of 1
Screening, production and biochemical characterization of a new fibrinolytic en...
Silva, Germana Michelle de Medeiros e; Bezerra, Raquel Pedrosa; Teixeira, J. A.; Silva, Flávio Oliveira; Correia, Juliana Mendes; Porto, Tatiana SouzaThrombosis is a pathophysiological disorder caused by accumulation of fibrin in the blood. Fibrinolytic proteases with potent thrombolytic activity have been produced by diverse microbial sources. Considering the microbial biodiversity of the Amazon region, this study aimed at the screening, production and biochemical characterization of a fibrinolytic enzyme produced by Streptomyces sp. isolated from Amazonian...
Fibrinolytic protease production by new Streptomyces sp. DPUA 1576 from amazon ...
Silva, Germana Michelle de Medeiros e; Bezerra, Raquel P.; Teixeira, J. A.; Porto, Tatiana Souza; Lima-Filho, José. L.; Porto, Ana L. F.Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 22 factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentra...
Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-p...
Silva, Germana Michelle de Medeiros e; Marques, Daniela Araújo Viana; Porto, Tatiana Souza; Lima Filho, José L.; Teixeira, J. A.Extraction of fibrinolytic protease from Streptomyces sp. DPUA1576 fermentation broth was performed using polyethylene glycol (PEG) – phosphate salts aqueous two phase system (ATPS). The influence of pH, PEG molar mass, PEG and phosphate concentrations on fibrinolytic protease partition coefficient (K) were evaluated by 24 full factorial design. Fibrinolytic protease partitioned preferentially to the (PEG)-rich...