3 documents found, page 1 of 1
Cytokine gene expression and molecular detection of Mycobacterium avium subspec...
Schwarz, David G. G.; Pietralonga, Pricila A. G.; Souza, Marina C. C.; Carvalho, Isabel A.; Cruzeiro, Rosyane S.; Malaquias, Juaci V.Mycobacterium avium subespécie paratuberculosis (MAP) pode infectar ruminantes e permanecer subclínica por longos períodos nos rebanhos. A identificação de órgãos mais susceptíveis à infecção e a avaliação da expressão das citocinas no local da infecção são importantes para compreender a patogênese de MAP. Neste estudo foi avaliada a probabilidade de detecção de DNA de MAP e a expressão de citocinas em órgãos d...
Escherichia coli from clinical mastitis: serotypes and virulence factors
Fernandes, José Benedito C.; Zanardo, Larissa G.; Galvão, Newton N.; Carvalho, Isabel A.; Nero, Luis Augusto; Moreira, Maria Aparecida S.In the current study, the virulence factors in Escherichia coli isolates from bovine mastitis were investigated, and the connection between these factors and infection was evaluated using phenotypic and genotypic analyses. Twenty-seven E. coli isolates were analyzed, and 2 were shown to produce verotoxin. All isolates had the ability to produce biofilms, although at different levels. One isolate was found to be...
Diagnosis of paratuberculosis in cattle: microbiological culture, serology and PCR
Carvalho, Isabel A.; Campos, Vinicius E. B.; Souza, Iana M.; Zanardo, Larissa G.; Filho, Jose D. Ribeiro; Gomes, Marcos J. P.; Moreira, Maria A. S.The aim of this study was to confirm clinical diagnosis of paratuberculosis in two cows showing suggestive clinical signs of the disease. Based on clinical signs, in culture and in IS900 PCR results from the individual milk samples it was possible to diagnose paratuberculosis in the cows studied.The aim of this study was to confirm clinical diagnosis of paratuberculosis in two cows showing suggestive clinical s...