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Effects of outdoor and indoor cultivation on the polar lipid composition and an...

Couto, Daniela; Conde, Tiago A.; Melo, Tânia; Neves, Bruna; Costa, Margarida; Cunha, Pedro; Guerra, Inês; Correia, Nádia; Silva, Joana T.; Pereira, Hugo

Nannochloropsis is a genus of eicosapentaenoic acid-rich microalgae with high levels of value-added polar lipids. However, the polar lipid composition of microalgal biomass is highly dependent on culture conditions (e.g., light or temperature), which are significantly different under indoor and outdoor culture conditions. In this study, we sought to investigate the plasticity of the polar lipid profile of a mar...


Operation regimes: A comparison based on Nannochloropsis oceanica biomass and l...

Guerra, Inês; Pereira, Hugo; Costa, Margarida; Silva, Joana T.; Santos, Tamara; Varela, João; Mateus, Marília; Silva, Joana

Microalgae are currently considered to be a promising feedstock for biodiesel production. However, significant research efforts are crucial to improve the current biomass and lipid productivities under real outdoor production conditions. In this context, batch, continuous and semi-continuous operation regimes were compared during the Spring/Summer seasons in 2.6 m(3) tubular photobioreactors to select the most ...


Isolation, Identification and Biotechnological Applications of a Novel, Robust,...

Correia, Nádia; Pereira, Hugo; Silva, Joana T.; Santos, Tamára; Soares, Maria; Sousa, Carolina B.; Schüler, Lisa M.; Costa, Margarida; Varela, João

Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblysto...


Development of an organic culture medium for autotrophic production of Chlorell...

Machado, Adriana; Pereira, Hugo; Costa, Margarida; Santos, Tamára; Carvalho, Bernardo; Soares, Maria; Quelhas, Pedro; Silva, Joana T.; Trovão, Mafalda

Microalgal biomass has gained increasing attention in the last decade for various biotechnological applications, including human nutrition. Certified organic products are currently a growing niche market in which the food industry has shown great interest. In this context, this work aimed at developing a certified organic culture medium for the production of autotrophic Chlorella vulgaris biomass. A preliminary...


Isolation, identification and biotechnological applications of a novel, robust,...

Correia, Nádia; Pereira, Hugo; Silva, Joana T.; Santos, Tamara; Soares, Maria; Sousa, Carolina B.; Schüler, Lisa Maylin; Costa, Margarida

Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblysto...


Nannochloropsis oceanica cultivation in pilot-scale raceway ponds—from design t...

Cunha, Pedro; Pereira, Hugo; Costa, Margarida; Pereira, João; Silva, Joana T.; Fernandes, Nuno; Varela, João; Silva, Joana; Simões, Manuel

Raceways ponds are the microalgal production systems most commonly used at industrial scale. In this work, two di erent raceway configurations were tested under the same processing conditions to compare their performance on the production of Nannochloropsis oceanica. Biomass productivity, biochemical composition of the produced biomass, and power requirements to operate those reactors were evaluated. Water dept...


Development of an organic culture medium for autotrophic production of chlorell...

Machado, Adriana; Pereira, Hugo; Costa, Margarida; Santos, Tamara; Carvalho, Bernardo; Soares, Maria; Quelhas, Pedro; Silva, Joana T.; Trovão, Mafalda

Microalgal biomass has gained increasing attention in the last decade for various biotechnological applications, including human nutrition. Certified organic products are currently a growing niche market in which the food industry has shown great interest. In this context, this work aimed at developing a certified organic culture medium for the production of autotrophic Chlorella vulgaris biomass. A preliminary...


Growth performance, biochemical composition and sedimentation velocity of Tetra...

Trovão, Mafalda; Pereira, Hugo; Silva, Joana; Páramo, Jaime; Quelhas, Pedro; Santos, Tamara; Silva, Joana T.; Machado, Adriana; Gouveia, Luísa

Biomass harvesting is one of the most expensive steps of the whole microalgal production pipeline. Therefore, the present work aimed to understand the effect of salinity on the growth performance, biochemical composition and sedimentation velocity of Tetraselmis sp. CTP4, in order to establish an effective low-cost pilot-scale harvesting system for this strain. At lab scale, similar growth performance was obtai...


Industrial production of Phaeodactylum tricornutum for CO2 mitigation: biomass ...

Quelhas, Pedro M.; Trovao, Mafalda; Silva, Joana T.; Machado, Adriana; Santos, Tamara; Pereira, Hugo; Varela, João; Simoes, Manuel; Silva, Joana L.

The photosynthetic efficiency (PE) and potential of Phaeodactylum tricornutum for CO2 mitigation in industrial tubular photobioreactors (PBRs) of different volumes were evaluated. A preliminary assay was performed at lab-scale to optimize the salt concentration of the culture medium. Interestingly, salinity did not affect the growth of P. tricornutum at concentrations of 2.5, 5, 10, and 20 g L-1. Higher volumet...


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