13 documents found, page 1 of 2
Towards a machine learning framework for predicting MicroRNAs involved in Candi...
Miranda, Tiago; Ferraz, Filipa; Henriques, Mariana; Silva, Sónia Carina; Gonçalves, Bruna FernandesInfections caused by Candida species present significant clinical challenges, leading to high morbidity and mortality rates. Emerging research suggests that microRNAs (miRNAs) may serve as valuable biomarkers for diagnosing and treating candidiasis, although the field is still developing. The scarcity of reported miRNAs associated with candidiasis stems from the complexities and costs of screening numerous pote...
Comprehensive molecular characterization of anti-EFG1 2'OMe effects on C. albic...
Silva, Diana; Silva, Sónia Carina; Henriques, Mariana; Araújo, Daniela; Gonçalves, Bruna FernandesCandidiasis is a fungal infection primarily caused by Candida albicans. It affects millions of people worldwide and poses significant clinical challenges, resulting in high morbidity and mortality rates, largely due to increasing resistance to conventional antifungal therapies resulting from their widespread use [1]. Innovative therapies, such as antisense oligonucleotides (ASOs), are being explored to address ...
Exploring bioinformatics tools to characterize a new regulator of Candida glabr...
Silva, Sónia Carina; Henriques, Mariana; Gonçalves, Bruna FernandesCandida glabrata is a clinically relevant human pathogen with ability to form high recalcitrant biofilms, which produce an extracellular matrix with suggestive virulent and protective roles. Thus, the elucidation of the matrix composition and regulation is crucial to disclose the matrix role in C. glabrata pathogenesis. As such, this study aimed to characterize, with various bioinformatics tools, a new C. glabr...
Biofilm matrix regulation by Candida glabrata Zap1 under acidic conditions: tra...
Gonçalves, Bruna Fernandes; Pires, Diana Priscila Penso; Fernandes, L.; Pacheco, Miguel; Ferreira, Tiago; Osório, Hugo; Soares, Ana RaquelThe vaginal acidic environment potentiates the formation of Candida glabrata biofilms, leading to complicated and recurrent infections. Importantly, the production of matrix is known to contribute to the recalcitrant features of Candida biofilms. In this study, we reveal that Zap1 regulates the matrix of C. glabrata acidic biofilms and analyzed the modulation of their transcriptome (by microarrays) and matrix p...
Sfl1 is required for Candida albicans biofilm formation under acidic conditions
Gonçalves, Bruna Fernandes; Barbosa, Ana Teresa Moutinho; Soares, Ana Raquel; Henriques, Mariana; Silva, Sónia CarinaCandida albicans is a common Candida species, responsible for infections in various anatomical sites under different environmental conditions, aggravated in the presence of its biofilms. As such, this study aimed to reveal the regulation of C. albicans biofilms under acidic conditions by the transcription factor Sfl1, whose role on biofilm formation is unclear. For that, microbiologic and transcriptomic analyse...
Vapor-phase of essential oils as a promising solution to prevent Candida vagina...
Fernandes, Liliana Sousa; Gonçalves, Bruna Fernandes; Costa, Raquel; Fernandes, Ângela; Gomes, Ana; Nogueira-Silva, Cristina; Silva, Sónia CarinaBackground: Vulvovaginal candidiasis (VVC) is a disease with high incidence, a huge impact on the quality of life and health of women, and which represents a great challenge to treat. The growing need to apply antifungal intensive therapies have contributed to an emergence of drug-resistant Candida strains. Thus, effective therapeutic options, to meet the antifungal-resistance challenge and to control high resi...
Vulvovaginal candidiasis and asymptomatic vaginal colonization in Portugal: Epi...
Fernandes, Ângela; Azevedo, Nuno Miguel Morais; Valente, Andreia; Dias, Marisol; Gomes, Ana; Nogueira-Silva, Cristina; Henriques, MarianaVulvovaginal candidiasis (VVC) has been identified as a global issue of concern due to its clinical, social and economic implications. The emerging relevance of VVC makes it crucial to increase the knowledge on its epidemiological and etiological features in order to improve its prevention and treatment. Thus, this study aimed to reveal the incidence, microbiology, antifungal pattern and risk factors of VVC in ...
Recurso a oligómeros antisense para controlar a formação de biofilme de Candida...
Araújo, Daniela Eira; Gonçalves, Bruna Fernandes; Vilas-Boas, Diana Patrícia Andrade; Rodrigues, Maria Elisa Costa; Henriques, MarianaA espécie de Candida albicans é uma das principais causas de candidíase e a sua patogenicidade é suportada por um conjunto de fatores de virulência, incluindo a capacidade de formar biofilme. O biofilme é um fenómeno sequencial regulado por uma complexa rede genética que envolve vários fatores de transcrição (EFG1, BRG1and ROB1). O aumento da multirresistência de C. albicans é acompanhado pela escassez de novos...
Hormones modulate Candida vaginal isolates biofilm formation and decrease their...
Gonçalves, Bruna Fernandes; Azevedo, Nuno Miguel Morais; Henriques, Mariana; Silva, Sónia CarinaVulvovaginal candidiasis (VVC) is an infection usually caused by Candida albicans and increasingly by Candida glabrata, which has an intrinsically high resistance to commonly used antifungals. Candida species possess virulence factors that contribute to VVC development, as the ability to form biofilms in vaginal walls and intrauterine devices. It is known that VVC is promoted by conditions that increase the hor...
Student skill development with the real world: Analyzing tert-Butyl alcohol con...
Gonçalves, Bruna Fernandes; Botelho, Gabriela; Medeiros, Maria José; Smith, Michael JohnIn the activity described in this communication, the authors present a simple experiment that can be implemented with moderate operational costs and that allows students to acquire the manipulative skills necessary for chromatographic analysis of a familiar fuel that still plays a fundamental role in providing energy for transport of passengers and goods. The use of gasoline formulations in a laboratory activit...