7 documents found, page 1 of 1
Conditioned medium as a strategy for human stem cells chondrogenic differentiation
Silva, M. L. Alves da; Pinto, A. R.; Martins, A.; Correlo, V. M.; Sol, P. C.; Bhattacharya, Mrinal; Faria, Susana; Reis, R. L.; Neves, N. M.Paracrine signalling from chondrocytes has been reported to increase the synthesis and expression of cartilage extracellular matrix (ECM) by stem cells. The use of conditioned medium obtained from chondrocytes for stimulating stem cells chondrogenic differentiation may be a very interesting alternative for moving into the clinical application of these cells, as chondrocytes could be partially replaced by stem c...
Indirect co-cultures of stem cells with chondrocytes for cartilage tissue engin...
Silva, M. L. Alves da; Martins, A.; Pinto, A. R.; Monteiro, N.; Reis, R. L.; Neves, N. M.Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013); Mesenchymal Stem Cells (MSCs) have been recognized for their ability to differentiate into cells of different tissues such as bone, cartilage or adipose tissue, and therefore might be of interest for potential therapeutic strategies. These cells are induced to differentiate by growth factors supplementation in cultur...
Tissue engineering as a remarkable tool for cartilage repair
Silva, M. L. Alves da; Reis, R. L.; Neves, N. M.Articular cartilage is a very specialized tissue with outstanding load-bearing capacity. It consists mainly of a dense extracellular matrix (ECM) with chondrocytes embedded on it. Cartilage has very low capacity of self-repair and regeneration after traumatic, degenerative or inflammatory injury. Current available surgical treatments for cartilage repair present several drawbacks, such as possible implant rejec...
Influence of conditioned medium over the chondrogenic differentiation of adult ...
Silva, M. L. Alves da; Pinto, A. R.; Silva, Vitor Manuel Correlo da; Sol, P.; Bhattacharya, Mrinal; Faria, Susana; Reis, R. L.; Neves, N. M.
Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment...
Silva, M. L. Alves da; Crawford, Aileen; Mundy, Jenifer; Correlo, V. M.; Sol, P.; Bhattacharya, Mrinal; Hatton, Paul V.; Reis, R. L.; Neves, N. M.Naturally derived polymers have been extensively used in scaffold production for cartilage tissue engineering. The present work aims to evaluate and characterize extracellular matrix (ECM) formation in two types of chitosan-based scaffolds, using bovine articular chondrocytes (BACs). The influence of these scaffolds’ porosity, as well as pore size and geometry, on the formation of cartilagineous tissue was stud...
Cartilage tissue engineering using electrospun PCL nanofiber meshes and MSCs
Silva, M. L. Alves da; Martins, A.; Costa-Pinto, A. R.; Costa, P.; Faria, Susana; Gomes, Manuela E.; Reis, R. L.; Neves, N. M.Mesenchymal stem cells (MSCs) have been recognized for their ability to differentiate into cells of different tissues such as bone, cartilage, or adipose tissue, and therefore are of great interest for potential therapeutic strategies. Adherent, colony-forming, fibroblastic cells were isolated from human bone marrow aspirates, from patients undergoing knee arthroplasties, and the MSCs phenotype characterized by...
Melt-based compression-molded scaffolds from chitosan-polyester blends and comp...
Correlo, V. M.; Boesel, L. F.; Pinho, Elisabete D.; Pinto, A. R.; Silva, M. L. Alves da; Bhattacharya, Mrinal; Mano, J. F.; Neves, N. M.; Reis, R. L.Blends of chitosan and synthetic aliphatic polyesters (polybutylene succinate, polybutylene succinate adipate, polycaprolactone, and polybutylene terepthalate adipate) were compounded with and without hydroxyapatite, a bioactive mineral filler known to enhance osteoconduction. The blends and composites were compression molded with two different granulometric salt sizes (63–125 lm and 250–500 lm) having differen...