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Proteome dynamics of the cotyledonary haustorium and endosperm in the course of...
Nascimento, José Roberto da Silva; Mélo Neto, Domingos Ferreira; Coutinho, Ítalo C.; Domont, Gilberto B.; Nogueira, Fábio C. S.; Campos, Francisco A. P.The role of the cotyledonary haustorium (CH) in the mobilization of nutrient reserves in the endosperm of species of the palm family Arecaceae is a moot question. To shed light on this matter, we present here an analysis of the quantitative proteome changes associated with four developmental stages of CH and three of endosperm during germination. Together, a total of 1965 proteins were identified, being 1538 in...
Differential expression of cysteine peptidase genes in the inner integument and...
Rocha, Antônio J.; Soares, Emanoella L.; Costa, José H.; Costa, Washington L. G.; Soares, Arlete A.; Nogueira, Fábio C. S.; Domont, Gilberto B.In several plant tissues, programmed cell death (PCD) is mediated by the combined action of cysteine peptidases, namely KDEL-tailed cysteine peptidases (KDEL-CysEP) and vacuolar processing enzymes (VPE). Here, we performed a search of the draft genome of Jatropha curcas L. (Euphorbiaceae) and identified 2 genes for KDEL-CysEP (Jc-CysEP1 and Jc-CysEP2) and 3 genes for VPE (Jc-βVPE, Jc-γVPE and Jc-ıVPE) and deter...
Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds du...
Nogueira, Fábio C. S.; Palmisano, Giuseppe; Soares, Emanoella L.; Shah, Mohibullah; Soares, Arlete A.; Roepstorff, Peter; Campos, Francisco A. P.In this study, we performed a proteomic analysis of nucellus from two developmental stages of Ricinus communis seeds by a GeLC-MS/MS approach, using of a high resolution orbi trap mass spectrometer, which resulted in the identification of a total of 766 proteins that were grouped into 553 protein groups. The distribution of the identified proteins in stages III and IV into different Gene Ontology categories was...