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Portuguese wild grapevine genome re-sequencing (Vitis vinifera sylvestris)

Ramos, Miguel J. N.; Coito, João L.; Faísca-Silva, David; Cunha, Jorge; Costa, Maria Manuela Ribeiro; Amâncio, Sara; Rocheta, Margarida

The first genome of Vitis vinifera vinifera (PN40024), published in 2007, boosted grapevine related studies. While this reference genome is a suitable tool for the overall studies in the field, it lacks the ability to unveil changes accumulated during V. v. vinifera domestication. The subspecies V. v. sylvestris preserves wild characteristics, making it a good material to provide insights into V. v. vinifera do...


RNA editing in inflorescences of wild grapevine unveils association to sex and ...

Ramos, Miguel J. N.; Faísca-Silva, David; Coito, João L.; Cunha, Jorge; Silva, Helena Gomes; Viegas, Wanda; Costa, Maria Manuela Ribeiro; Amâncio, Sara

RNA editing challenges the central dogma of molecular biology, by modifying the genetic information at the transcription level. Recent reports, suggesting increased levels of RNA editing in plants, raised questions on the nature and dynamics of such events during development. We here report the occurrence of distinct RNA editing patterns in wild Vitis flowers during development, with twelve possible RNA editing...


Portuguese wild grapevine genome re-sequencing (Vitis vinifera sylvestris)

Ramos, Miguel J.N.; Coito, João L.; Faísca-Silva, David; Cunha, Jorge; Costa, M.Manuela R.; Amâncio, Sara; Rocheta, Margarida

The first genome of Vitis vinifera vinifera (PN40024), published in 2007, boosted grapevine related studies. While this reference genome is a suitable tool for the overall studies in the field, it lacks the ability to unveil changes accumulated during V. v. vinifera domestication. The subspecies V. v. sylvestris preserves wild characteristics, making it a good material to provide insights into V. v. vinifera do...


Response of mycorrhizal Touriga Nacional variety grapevines to high temperature...

Nogales, Amaia; Ribeiro, Hugo; Nogales-Bueno, Julio; Hansen, Lee D.; Gonçalves, Elsa F.; Coito, João L.; Rato, Ana Elisa; Peixe, Augusto; Viegas, Wanda

Heat stress negatively affects several physiological and biochemical processes in grapevine plants. In this work, two new methods, calorespirometry, which has been used to determine temperature adaptation in plants, and near-infrared (NIR) spectroscopy, which has been used to determine several grapevine-related traits and to discriminate among varieties, were tested to evaluate grapevine response to high temper...


Vitis flower sex specification acts downstream and independently of the ABCDE m...

Coito, João L.; Silva, Helena Sofia Gomes; Ramos, Miguel J. N.; Montez, Miguel; Cunha, Jorge; Amâncio, Sara; Costa, Maria M. R.; Rocheta, Margarida

The most discriminating characteristic between the cultivated Vitis vinifera subsp. vinifera and the wild-form Vitis vinifera subsp. sylvestris is their sexual system. Flowers of cultivars are mainly hermaphroditic, whereas wild plants have female and male individuals whose flowers follow a hermaphroditic pattern during early stages of development and later develop non-functional reproductive organs. In angiosp...


Physiological and agronomical response to environmental fluctuations of two por...

Carvalho, Luísa C.; Coito, João L.; Gonçalves, Elsa F.; Lopes, C.M.; Amâncio, Sara

Extensive agricultural losses are attributed to heat, often combined with drought. These abiotic stresses occur in the field simultaneously, namely in areas with Mediterranean climate, where grapevine traditionally grows. The available scenarios for climate change suggest an increase in the frequency of heat waves and severe drought events in summer, also affecting the South of Portugal. In this work we monitor...


Physiological and agronomical responses to environmental fluctuations of two po...

Carvalho, Luísa C.; Coito, João L.; Gonçalves, Elsa F.; Lopes, C.M.; Amâncio, Sara

Extensive agricultural losses are attributed to heat, often combined with drought. These abiotic stresses occur in the field simultaneously, namely in areas with Mediterranean climate, where grapevine traditionally grows. The available scenarios for climate change suggest an increase in the frequency of heat waves and severe drought events in summer, also affecting the South of Portugal. In this work we monitor...


VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distingui...

Coito, João L.; Ramos, Miguel J. N.; Cunha, Jorge; Silva, Helena Sofia Gomes; Amâncio, Sara; Costa, Maria Manuela Ribeiro; Rocheta, Margarida

Vitis vinifera vinifera is a hermaphrodite subspecies, while its ancestor, Vitis vinifera sylvestris, is dioecious. We have identified two genes that together allow the discrimination between male, female and hermaphrodite Vitis plants. The sex locus region on chromosome 2 was screened resulting in the discovery of a new gene, VviFSEX. The same screening revealed another gene, VviAPRT3, located in the sex regio...


Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in...

Carvalho, Luísa C.; Silva, Marilia; Coito, João L.; Rocheta, Margarida P.; Amâncio, Sara

Widespread agricultural losses attributed to drought, often combined with high temperatures, frequently occur in the field, namely in Mediterranean climate areas, where the existing scenarios for climate change indicate an increase in the frequency of heat waves and severe drought events in summer. Grapevine (Vitis vinifera L.) is the most cultivated fruit species in the world and the most valuable one and is a...


VviAPRT3 and VviFSEX: two genes involved in sex specification able to distingui...

Coito, João L.; Ramos, Miguel J.N.; Cunha, Jorge; Silva, Helena G.; Amâncio, Sara; Costa, Maria M.R.; Rocheta, Margarida

Vitis vinifera vinifera is a hermaphrodite subspecies, while its ancestor, Vitis vinifera sylvestris, is dioecious. We have identified two genes that together allow the discrimination between male, female and hermaphrodite Vitis plants. The sex locus region on chromosome 2 was screened resulting in the discovery of a new gene, VviFSEX. The same screening revealed another gene, VviAPRT3, located in the sex regio...


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