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Exosomal aβ-binding proteins identified by “in silico” analysis represent putat...
Martins, Tânia Soares; Marçalo, Rui; Ferreira, Maria; Vaz, Margarida; Silva, Raquel M.; Rosa, Ilka Martins; Vogelgsang, Jonathan; Wiltfang, JensThe potential of exosomes as biomarker resources for diagnostics and even for therapeutics has intensified research in the field, including in the context of Alzheimer´s disease (AD). The search for disease biomarkers in peripheral biofluids is advancing mainly due to the easy access it offers. In the study presented here, emphasis was given to the bioinformatic identification of putative exosomal candidates fo...
NMR metabolomics to study the metabolic response of human osteoblasts to non-po...
Araújo, Rita; Carneiro, Tatiana J.; Marinho, Paula; Costa, Marisa Maltez da; Roque, Ana; Silva, Odete A. B. da Cruz e; Fernandes, Maria HelenaUntargeted nuclear magnetic resonance (NMR) metabolomics was employed, for the first time to our knowledge, to characterize the metabolome of human osteoblast (HOb) cells and extracts in the presence of non-poled or negatively poled poly-L-lactic acid (PLLA). The metabolic response of these cells to this polymer, extensively used in bone regeneration strategies, may potentially translate into useful markers ind...
CD81 promotes a migratory phenotype in neuronal-like cells
Martins, Soraia A.; Correia, Patrícia D.; Dias, Roberto A.; Silva, Odete A. B. da Cruz e; Vieira, Sandra I.Tetraspanins, such as CD81, can form lateral associations with each other and with other transmembrane proteins. These interactions may underlie CD81 functions in multiple cellular processes, such as adhesion, morphology, migration, and differentiation. Since CD81's role in neuronal cells' migration has not been established, we here evaluated effects of CD81 on the migratory phenotype of SH-SY5Y neuroblastoma c...
Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throu...
Serrano, Joana B.; Martins, Filipa; Sousa, João Carlos; Pereira, Cátia D.; Pelt, Ans M. M. van; Rebelo, Sandra; Silva, Odete A. B. da Cruz eSpermatogenesis comprises highly complex differentiation processes. Nuclear envelope (NE) proteins have been associated with these processes, including lamins, lamina-associated polypeptide (LAP) 2 and the lamin B-receptor. LAP1 is an important NE protein whose function has not been fully elucidated, but several binding partners allow predicting putative LAP1 functions. To date, LAP1 had not been associated wit...
Not so pseudo: the evolutionary history of protein phosphatase 1 regulatory sub...
Korrodi-Gregório, Luís; Abrantes, Joana; Muller, Thorsten; Melo-Ferreira, José; Marcus, Katrin; Silva, Odete A. B. da Cruz e; Fardilha, MargaridaBackground: Pseudogenes are traditionally considered “dead” genes, therefore lacking biological functions. This view has however been challenged during the last decade. This is the case of the Protein phosphatase 1 regulatory subunit 2 (PPP1R2) or inhibitor-2 gene family, for which several incomplete copies exist scattered throughout the genome. Results: In this study, the pseudogenization process of PPP1R2 was...
An intriguing shift occurs in the novel protein phosphatase 1 binding partner, ...
Korrodi-Gregório, Luís; Lopes, Ana Margarida; Esteves, Sara L. C.; Afonso, Sandra; Matos, Ana Lemos de; Lissovsky, Andrey A.T-complex testis expressed protein 1 domain containing 4 (TCTEX1D4) contains the canonical phosphoprotein phosphatase 1 (PPP1) binding motif, composed by the amino acid sequence RVSF. We identified and validated the binding of TCTEX1D4 to PPP1 and demonstrated that indeed this protein is a novel PPP1 interacting protein. Analyses of twenty-one mammalian species available in public databases and seven Lagomorpha...
Identification and characterization of two distinct PPP1R2 isoforms in human sp...
Korrodi-Gregório, Luís; Ferreira, Mónica; Vintém, Ana Paula; Wenjuan Wu; Muller, Thorsten; Marcus, Katrin; Vijayaraghavan, SrinivasanBackground: Protein Ser/Thr Phosphatase PPP1CC2 is an alternatively spliced isoform of PPP1C that is highly enriched in testis and selectively expressed in sperm. Addition of the phosphatase inhibitor toxins okadaic acid or calyculin A to caput and caudal sperm triggers and stimulates motility, respectively. Thus, the endogenous mechanisms of phosphatase inhibition are fundamental for controlling sperm function...
A importância do mecanismo de “splicing” alternativo para a identificação de no...
Fardilha, Margarida; Silva, Odete A. B. da Cruz e; Silva, Edgar F. da Cruz eA motilidade progressiva dos espermatozóides é um factor essencial para a sua fertilidade. Ao nível molecular, este processo depende da fosforilação de proteínas ainda não identificadas, uma vez que o tratamento de espermatozóides imóveis com agentes moduladores da fosforilação proteica, incluindo inibidores específicos de proteínas fosfatases, induz a sua motilidade. Os nossos estudos anteriores indicam que a ...