71 documents found, page 1 of 8
Cryogenic, but not hypothermic, preservation disrupts the extracellular matrix ...
Ribeiro, Sara Cristina Freitas; Carvalho, A. F.; Rodrigues, Daniel Barreira; Martins, L.; Pires, R. A.; Mendes, V. M.; Manadas, B.; Jarnalo, M.Cell sheet (CS)-based approaches hold significant potential for tissue regeneration, relying on the extracellular matrix (ECM) for success. Like in native tissues, the ECM provides structural and biochemical support for cellular homeostasis and function. Effective preservation strategies that maintain ECM integrity are critical to enhance the therapeutic potential of CS-based approaches. While cryogenic and hyp...
Prevascularized spongy-like hydrogels maintain their angiogenic potential after...
Ribeiro, Sara Cristina Freitas; Moreira, H.; da Silva, Lucília Pereira; Noro, Jennifer Martins; Sampaio-Marques, B.; Ludovico, Paula; Jarnalo, M.The chronic shortage of organs and tissues for transplantation represents a dramatic burden on healthcare systems worldwide. Tissue engineering offers a potential solution to address these shortages, but several challenges remain, with prevascularization being a critical factor for in vivo survival and integration of tissue engineering products. Concurrently, a different challenge hindering the clinical impleme...
Modelling the complex nature of the tumor microenvironment: 3D tumor spheroids ...
Rodrigues, Daniel Barreira; Reis, R. L.; Pirraco, Rogério P.Cancer remains a serious burden in society and while the pace in the development of novel and more effective therapeutics is increasing, testing platforms that faithfully mimic the tumor microenvironment are lacking. With a clear shift from animal models to more complex in vitro 3D systems, spheroids emerge as strong options in this regard. Years of development have allowed spheroid-based models to better repro...
How are natural-based polymers shaping the future of cancer immunotherapy—A review
Rodrigues, Daniel Barreira; Reis, R. L.; Pirraco, Rogério P.With the increasing knowledge of cancer pathophysiology, new therapeutics based on the modulation of the immune system have been developed, overcoming many of the disadvantages of traditional pharmaceuticals. Several immunotherapy systems have in fact become the preferred treatments to tackle particular types of cancer. Despite these impressive clinical results, issues, such as biomolecule susceptibility to pro...
Stromal vascular fraction obtained from subcutaneous adipose tissue: ex-obese a...
Frias, Frias; Matos, Beatriz; Jarnalo, Mariana; Freitas-Ribeiro, Sara; Reis, R. L.; Pirraco, Rogério P.; Horta, RicardoIntroduction. Human adipose tissue contains a heterogeneous and synergistic mixture of cells called stromal vascular fraction (SVF) with highly proliferative and angiogenic properties, conferring promising applicability in the field of regenerative medicine. This study aims to investigate if age, body mass index (BMI), history of obesity and massive weight loss, and harvest site are related to SVF cell marker e...
Human adipose-derived mesenchymal stem cells laden in gellan gum spongy-like hy...
Alheib, Omar; da Silva, Lucília Pereira; da Silva Morais, Alain; Mesquita, Katia Almeida; Pirraco, Rogério P.; Reis, R. L.; Correlo, V. M.Background: volumetric muscle loss (VML) is a traumatic massive loss of muscular tissue which frequently leads to amputation, limb loss, or lifetime disability. The current medical intervention is limited to autologous tissue transfer, which usually leads to non-functional tissue recovery. Tissue engineering holds a huge promise for functional recovery. Methods: in this work, we evaluated the potential of h...
How are natural-based polymers shaping the future of cancer immunotherapy: a re...
Rodrigues, Daniel Barreira; Reis, R. L.; Pirraco, Rogério P.With the increasing knowledge of cancer pathophysiology, new therapeutics based on the modulation of the immune system have been developed, overcoming many of the disadvantages of traditional pharmaceuticals. Several immunotherapy systems have in fact become the preferred treatments to tackle particular types of cancer. Despite these impressive clinical results, issues, such as biomolecule susceptibility to pro...
From its nature to its function: marine-collagen-based-biomaterials for hard ti...
Carlos, Gabriela Soares Diogo; Pirraco, Rogério P.; Reis, R. L.; Silva, Tiago H.Rapidly growing demand for collagen-based therapeutic applications requires a great amount of collagen stock. Commercial collagen is mainly confined to mammalian sources, which have concerns about zoonotic disease transfer and, additionally, the problem of terrestrial animals' overexploitation, which, even so, does not meet the crescent demand for collagen. The extraction of collagen from marine organisms, incl...
Spongy-like hydrogels prevascularization with the adipose tissue vascular fract...
Moreira, Helena R.; Rodrigues, Daniel Barreira; Ribeiro, Sara Freitas; Silva, Lucília Pereira; Morais, Alain da S.; Jarnalo, Mariana; Horta, RicardoIn vitro prevascularization is one of the most explored approaches to foster engineered tissue vascularization. We previously demonstrated a benefit in tissue neovascularization by using integrin-specific biomaterials prevascularized by stromal vascular fraction (SVF) cells, which triggered vasculogenesis in the absence of extrinsic growth factors. SVF cells are also associated to biological processes important...
A novel method for the preparation of poly (Acrylamide-co-Acrylonitrile) upper ...
Ling, Yi; Chen, Liuyuchen; Huang, Mingjun; Zhou, Cheng; Yang, Liming; Niu, Hejingying; Su, Li; Yang, Yuejiao; Pirraco, Rogério P.; Reis, R. L.In an attempt to find a potential application of cell culture harvesting, a novel method for the preparation of an upper critical solution temperature (UCST) thermosensitive hydrogel was studied. An electron accelerator was used as the electron beam (EB) radiation source, and acrylamide (AAm) was first grafted onto the pre-irradiated polypropylene (PP) sheet. Then, the grafting layer of poly (acrylamide-co-acry...