A novel trypsin inhibitor, named RcTI, was purified from castor bean cake (Ricinus communis L.) by heat treatment followed by chromatography on anhydrotrypsin-Sepharose 4B and Resource Q. RcTI is a 14 kDa competitive inhibitor with pI 5.2 and a dissociation constant (Ki) of 1.9 × 10−5 mM. The amino-terminal sequence showed similarity with a 2S sulfur-rich seed storage protein (83%) and napin-like protein (48%)....
The castor bean (Ricinus communis L.) culture is of great socioeconomic importance because of its seeds, which are used mainly for production of oil that can be used for various industrial purposes. The oil extraction generates a by-product of high nutritional value known as the castor cake. However, the presence of toxic and allergenic compounds hinders the use of this residue to feeding source. Another way to...
SILVA, Rodolpho Glauber Guedes. Respostas fisiológicas e bioquímicas do feijão-de-corda [Vigna unguiculata (L.) Walp.] submetido ao estresse hídrico, infectado com o vírus do mosaico severo do caupi, e sob pressão dos dois estresses combinados. 2016. 137 f. Tese (Doutorado em bioquímica)- Universidade Federal do Ceará, Fortaleza-CE, 2016.; Cowpea (Vigna unguiculata) is a legume (Fabaceae) with high nutritional ...