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The chlorophyll fluoroscope, a device to observe the in vivo emission of chloro...

Serôdio, João

In vivo chlorophyll fluorescence has long been known to be intimately related to photosynthesis, being the basis of widespread and sophisticated instrumentation. Although easily observed in plant extracts or using epifluorescence or confocal microscopes, chlorophyll fluorescence is seldom observed in macroscopic samples, such as plant leaves of macroalgae thalli. This work presents a 'chlorophyll fluoroscope', ...


Redox homeostasis disclosed in the saltmarsh plant Halimione portulacoides upon...

Pereira, Patrícia; Pereira, Joana Luísa; Marques, Ana; Marques, Carlos; Brandão, Fátima; Cesário, Rute; Frankenbach, Silja; Serôdio, João

The saltmarsh plant Halimione portulacoides was shortly exposed to realistic levels of inorganic mercury (iHg) with the aim of investigating the adaptative processes of the roots and leaves regarding redox homeostasis, physiology, and Hg accumulation. Plants were collected at a contaminated (CONT) and a reference (REF) site to address the interference of contamination backgrounds. The influence of major abiotic...


Fruit photosynthesis: more to know about where, how and why

Garrido, Andreia Raquel Martins; Conde, Artur Jorge Silva; Serôdio, João; De Vos, Ric C. H.; Cunha, Ana

Not only leaves but also other plant organs and structures typically considered as carbon sinks, including stems, roots, flowers, fruits and seeds, may exhibit photosynthetic activity. There is still a lack of a coherent and systematized body of knowledge and consensus on the role(s) of photosynthesis in these “sink” organs. With regard to fruits, their actual photosynthetic activity is influenced by a range of...


Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeoda...

Pinhal, Mafalda; Serôdio, João; Frankenbach, Silja

Diatoms are photosynthetic organisms that are very important for the marine ecosystem and are the basis of primary production in these habitats. Knowing the importance of this group, this work aims to investigate if chemical stress affects the Epigenetic of diatoms. For that, it was verified the resistance of the microalgae Phaeodactylum tricornutum when expose to the antibiotic ciprofloxacin and to the metal c...


Cyanobacteria as candidates to support mars colonization: growth and biofertili...

Macário, Inês P. E.; Veloso, Telma; Frankenbach, Silja; Serôdio, João; Passos, Helena; Sousa, Clara; Gonçalves, Fernando J. M.; Ventura, Sónia P. M.

Cyanobacteria are indicated as organisms that can possibly support Mars colonization, contributing to the production of oxygen and other commodities therein. In this general context, the aim of this work was to evaluate the ability of three species of cyanobacteria (Anabaena cylindrica, Nostoc muscorum, and Arthrospira platensis) and a green microalga (Chlorella vulgaris) to grow using only the resources existi...


Cyanobacteria as Candidates to Support Mars Colonization: Growth and Biofertili...

Macário, Inês P. E.; Veloso, Telma; Frankenbach, Silja; Serôdio, João; Passos, Helena; Sousa, Clara; Gonçalves, Fernando J. M.; Ventura, Sónia P. M.

Cyanobacteria are indicated as organisms that can possibly support Mars colonization, contributing to the production of oxygen and other commodities therein. In this general context, the aim of this work was to evaluate the ability of three species of cyanobacteria (Anabaena cylindrica, Nostoc muscorum, and Arthrospira platensis) and a green microalga (Chlorella vulgaris) to grow using only the resources existi...


Are cyanobacteria a nearly immortal source of high market value compounds?

Macário, Inês P. E.; Veloso, Telma; Fernandes, Andreia P. M.; Martins, Margarida; Frankenbach, Silja; Serôdio, João; Gonçalves, Fernando J. M.

BACKGROUND: When the human population increases, so does the need to explore a wider range of feedstocks and biomasses, such as cyanobacteria. However, a deeper understanding of the growth patterns and pigment production is required to support the selection of the most beneficial species and conditions for industrial production. The growth and pigment production (i.e., chlorophyll a and C-phycocyanin) of three ...


Toxicity of recombinant necrosis and ethylene-inducing proteins (NLPs) from Neo...

Pour, Forough Nazar; Cobos, Rebeca; Coque, Juan José Rubio; Serôdio, João; Alves, Artur; Félix, Carina; Ferreira, Vanessa; Esteves, Ana Cristina

Neofusicoccum parvum is a fungal pathogen associated with a wide range of plant hosts. Despite being widely studied, the molecular mechanism of infection of N. parvum is still far from being understood. Analysis of N. parvum genome lead to the identification of six putative genes encoding necrosis and ethylene-inducing proteins (NLPs). The sequence of NLPs genes (NprvNep 1-6) were analyzed and four of the six N...


Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L. an ec...

Soares, Cristiano; Pereira, Ruth; Martins, Maria; Tamagnini, Paula; Serôdio, João; Moutinho-Pereira, José; Cunha, Ana; Fidalgo, Fernanda

This study aimed to assess the toxicity of glyphosate (GLY; 0, 10, 20 and 30?mg?kg-1) in Solanum lycopersicum L., particularly focusing on the photosynthetic metabolism. By combining ecophysiological, ultrastructural, biochemical and molecular tools, the results revealed that the exposure of tomato plants to GLY led to alterations in leaf water balance regulation [increasing stomatal conductance (gs) and decrea...


Effect of temperature on the phytotoxicity and cytotoxicity of Botryosphaeriace...

Nazar Pour, Forough; Ferreira, Vanessa; Félix, Carina; Serôdio, João; Alves, Artur; Duarte, Ana Sofia; Esteves, Ana Cristina

Botryosphaeriaceae fungi are phytopathogens and human opportunists. The influence of temperature on the phytotoxicity and cytotoxicity of culture filtrates of five Botryosphaeriaceae species was investigated. All culture filtrates of fungi grown at 25 °C were phytotoxic: symptoms were evaluated based on visual inspection of necrosis areas and on the maximum quantum yield of photosystem II, Fv/Fm. Diplodiacortic...


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