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Bioprocess to produce biostimulants/biofertilizers based on microalgae grown us...

Ferreira, Alice; Corrêa, D. O.; Ribeiro, Belina; Silva, Teresa Lopes da; Santos, Cláudia Marques dos; Acién, F. Gabriel; Gouveia, Luisa

ABSTRACT: In the present work, two downstream processes - high-pressure homogenization at 100 (HPH-100) and 1200 bar (HPH-1200), and enzymatic hydrolysis (EH) - were tested to produce biostimulant extracts from Tetradesmus obliquus grown in piggery wastewater at two concentrations (12.8 and 88.3 g/L). Extracts before and after centrifugation (C) were evaluated in four bioassays using garden cress (germination),...

Date: 2024   |   Origin: Repositório do LNEG

Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production

Fontes, Afonso; Francisco, Ricardo; Ferreira, Frederico Castelo; Faria, Nuno Torres; Marques, Susana; Reis, Alberto; Moura, Patrícia; Lukasik, Rafal M.

ABSTRACT: Microbial oils can be used as an alternative sustainable and renewable feedstock to fossil reserves for producing lubricants and polyurethane materials. Two oleaginous yeasts were grown on non-detoxified corn stover hydrolysate supplemented with corn steep liquor and mineral medium in shake flasks. Trichosporon oleaginosus DSM 11815 displayed the highest lipid production. This strain was further culti...

Date: 2024   |   Origin: Repositório do LNEG

Bioprocess to produce biostimulants/biofertilizers based on microalgae grown us...

Ferreira, Alice; Corrêa, Diego O.; Ribeiro, Belina; Silva, Teresa Lopes da; Marques-dos-Santos, Cláudia; Acién, F. Gabriel; Gouveia, Luisa

In the present work, two downstream processes - high-pressure homogenization at 100 (HPH-100) and 1200 bar (HPH-1200), and enzymatic hydrolysis (EH) - were tested to produce biostimulant extracts from Tetradesmus obliquus grown in piggery wastewater at two concentrations (12.8 and 88.3 g/L). Extracts before and after centrifugation (C) were evaluated in four bioassays using garden cress (germination), mung bean...


Bioprocess to produce biostimulants/biofertilizers based on microalgae grown us...

Ferreira, Alice; Corrêa, Diego O.; Ribeiro, Belina; Silva, Teresa Lopes da; Marques-dos-Santos, Cláudia; Gabriel Acién, F.; Gouveia, Luisa

In the present work, two downstream processes − high-pressure homogenization at 100 (HPH-100) and 1200 bar (HPH-1200), and enzymatic hydrolysis (EH) − were tested to produce biostimulant extracts from Tetradesmus obliquus grown in piggery wastewater at two concentrations (12.8 and 88.3 g/L). Extracts before and after centrifugation (C) were evaluated in four bioassays using garden cress (germination), mung bean...


Oleaginous Yeast Biorefinery: Feedstocks, Processes, Techniques, Bioproducts

Silva, Teresa Lopes da; Fontes, Afonso; Reis, Alberto; Silva, Carla; Gírio, Francisco

ABSTRACT: The world climate crisis has triggered the search for renewable energy sources. Oleaginous yeasts are a potential renewable source of biofuels. However, the yeast-derived biofuels cost is still non-competitive with the fossil fuel prices. To improve the sustainability of yeast-derived biofuels, it is necessary to valorize all yeast biomass fractions, an approach based on the biorefinery concept. This ...

Date: 2023   |   Origin: Repositório do LNEG

The Use of Oleaginous Yeasts and Microalgae Grown in Brewery Wastewater for Lip...

Dias, Carla; Santos, José A. L.; Reis, Alberto; Silva, Teresa Lopes da

ABSTRACT: Brewery wastewater has been proposed as an attractive low-cost substrate for microbial lipid production for oleaginous yeast and microalga with promising results. For each liter of beer produced, from 3 to 10 L of wastewater are generated which can be used as culture medium for autotrophic or heterotrophic metabolism. This strategy allows reducing the culture medium cost, as well as obtaining high lip...

Date: 2023   |   Origin: Repositório do LNEG

Effect of brewery effluent inhibitors on Rhodotorula toruloides NCYC 921 cells ...

Dias, Carla; Santos, J. A. L.; Reis, Alberto; Silva, Teresa Lopes da

ABSTRACT: The presence of inhibitor compounds in the culture medium can cause severe effects on the microorganisms cells. Brewery wastewaters present organic acids (acetic, propionic and butyric acids) which can severely affect yeast cells metabolism, when grown in pure cultures, although in mixed cultures they are able to develop. To understand the physiological changes on Rhodotorula toruloides (formerly Rhod...

Date: 2022   |   Origin: Repositório do LNEG

The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to...

Moniz, Patricia; Martins, Daniela; Oliveira, Ana Cristina; Reis, Alberto; Silva, Teresa Lopes da

ABSTRACT: Chrypthecodinium cohnii lipids have been almost exclusively used as a source of Docosahexaenoic acid (DHA). Such an approach wastes the remaining microalgal lipid fraction. The present work presents a novel process to produce C. cohnii biomass, using low-cost industrial by-products (raw glycerol and corn steep liquor), in a 7L-bioreactor, under fed-batch regime. At the end of the fermentation, the bio...

Date: 2022   |   Origin: Repositório do LNEG

Impact of brewery wastewater inhibitors in pure and mixed cultures of the yeast...

Dias, Carla; Santos, J. A. L.; Reis, Alberto; Silva, Teresa Lopes da

ABSTRACT: Brewery wastewater (BWW) is an appealing low-cost substrate for the production of single cell oils by oleaginous microorganisms. However, it may contain inhibitor compounds that may affect the microbial metabolism. This work investigated, for the first time, the presence of potential inhibitor compounds in primary brewery wastewater (PBWW) and secondary brewery wastewater (SBWW) for the pure and mixed...

Date: 2022   |   Origin: Repositório do LNEG

Cascading Crypthecodinium cohnii biorefinery: global warming potential and tech...

Silva, Carla; Moniz, Patricia; Oliveira, Ana Cristina; Vercelli, Samuela; Reis, Alberto; Silva, Teresa Lopes da

ABSTRACT: Prior to the commissioning of a new industrial biorefinery it is deemed necessary to evaluate if the new project will be beneficial or detrimental to climate change, one of the main drivers for the sustainable development goals (SDG) of the United Nations. In particular, how SDG 7, Clean and Efficient Energy, SDG 3, Good Health and Well Being, SDG 9, Industry Innovation and Infrastructure, and SDG 12,...

Date: 2022   |   Origin: Repositório do LNEG

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